Method for in vitro regeneration culture of true leaves of Chinese cabbage
A technology of in vitro regeneration and cultivation method, which is applied in the field of agriculture, can solve the problems of complex cultivation procedures and long cultivation period, and achieve the effect of simple cultivation method, low cost and short regeneration period
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Embodiment 1
[0039] 1) Material
[0040] Orange Chinese cabbage 06J28 (references, Deng Yongling, Zhang Lugang*, Dong Meiyun, Fan Aili, Hui Maixia, Zhang Mingke; Histological observations on regeneration of adventitious buds from petioled cotyledons of Chinese cabbage, Northwest Agricultural Journal, 2008, 17( 4): 238~243).
[0041] 2) method
[0042] The first step, aseptic vaccine culture
[0043] Select orange Chinese cabbage 06J28 seeds with full grains, and under sterile conditions, first sterilize the surface with 70% alcohol by volume for about 0.5-1min, then sterilize with 3% NaClO solution for 13-15min, and then use sterile water repeatedly Rinse 4 to 5 times, 5 minutes each time. Finally, the seeds were sown on the surface of 1 / 2 MS basic medium, and cultivated under the conditions of a temperature of 25±1°C, a light time of 16h / d, and a light intensity of 2000lx;
[0044] The second step is to select plants suitable for seedlings
[0045] About 9 to 10 days after sowing...
Embodiment 2
[0071] 1) Material
[0072] Common Chinese cabbage 92S105 (same as 92-S 105 ) (References, Ke Guilan, Song Yanzhi, Zhao Limin, Zhang Lugang, etc., Qinbai No. 4 Chinese Cabbage Breeding. Shaanxi Agricultural Science, 1996, No.4, 12-15).
[0073] 2) method
[0074] The first step, aseptic vaccine culture
[0075] Select common Chinese cabbage 92S105 seeds with full grains. Under aseptic conditions, first sterilize the surface with 70% alcohol by volume for about 0.5-1min, then sterilize with 3% NaClO solution for 13-15min, and then use Rinse with sterile water 4 to 5 times, 5 minutes each time. Finally, the seeds were sown flat on the surface of the conventional 1 / 2 MS basic medium, and cultivated under the conditions of a temperature of 25±1°C, a light time of 16h / d, and a light intensity of 2000lx;
[0076] The second step is to select plants suitable for seedlings
[0077] After culturing for 9-10 days and the seedlings grow upright, select the plants that grow robus...
Embodiment 3
[0088] 1) Material
[0089] Orange Chinese cabbage 01S1024 (References: Zhang Lugang, Hui Maixia, Zhang Mingke, Breeding of a new colorful Chinese cabbage variety "Jinguan 2". Journal of Northwest Agricultural Science 2007, 16 (1): 204-206).
[0090] 2) method
[0091] The first step, aseptic vaccine culture
[0092] Select orange Chinese cabbage 01S1024 seeds with full grains, and under aseptic conditions, first sterilize the surface with 70% alcohol by volume for about 0.5-1min, then sterilize with 3% NaClO solution for 13-15min, and then use Rinse with sterile water 4 to 5 times, 5 minutes each time. Finally, the seeds were sown on the surface of conventional 1 / 2 MS basic medium, and cultivated under the conditions of temperature 25±1°C, light time 16h / d, light intensity 2000lx;
[0093] The second step is to select plants suitable for seedlings
[0094] After cultivating for 9-10 days, when the seedlings grow upright, select the plants that grow robustly, and the f...
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