41 results about "Carbon utilization" patented technology

The invention belongs to the technical field of drought risk prediction and vegetation influence evaluation, and particularly discloses a vegetation-to-drought resource utilization coupling response strategy evaluation method, which comprises the following steps: acquiring target data; constructing a drought index according to the target data, and extracting a drought event; extracting vegetation total productivity, vegetation net level productivity and evapotranspiration abnormal data before and after the drought event, and calculating response intensity of vegetation variables; calculating the average variation of the light energy utilization efficiency, the carbon utilization efficiency and the moisture utilization efficiency; comprehensively considering the multivariable resistance-recovery state of the vegetation physiological process and the regulating variable of the resource utilization efficiency of the vegetation in response to drought stress, and analyzing the drought event resistance-recovery response capability of the vegetation. The method can more accurately and objectively understand the physiological mechanism difference of the vegetation to the drought stress response.

The invention provides a uracil production strain and a construction method and application thereof.According to the strain, on an E.coli UR14 genome by means of a CRIPSR / Cas9 gene editing technology, firstly, psuG genes, preTA genes, rutA genes and upp genes are knocked out, so that decomposition of uracil is blocked; then, a uridine phosphorylase gene udp and a pyrimidine-5 '-nucleotide nucleotidase gene ppnN are subjected to overexpression, and synthesis and accumulation of uracil are synergistically enhanced; and finally, overexpression of the ribose phosphate mutase gene pgm further enhances the conversion of a by-product ribose phosphate 1-precursor 5-ribose phosphate 1-pyrophosphate and improves the carbon utilization rate, and the obtained strain has good genetic stability and high fermentation yield, can stably produce uracil, and has wide application prospects.

The invention discloses a soil microorganism carbon utilization efficiency determination method, and relates to the technical field of carbon utilization efficiency determination, and the method comprises soil sample pre-culture, multi-source 18O isotope gradient labeling, dynamic gas monitoring, microorganism respiration rate dynamic correction, and microorganism growth rate and carbon utilization efficiency acquisition. By labeling multi-source isotopes and quantifying contributions of different oxygen sources to microorganism DNA synthesis, underestimation of the carbon utilization efficiency caused by a traditional single labeling method is avoided, the problem of limitation existing in the current soil microorganism carbon utilization efficiency determination process is solved, the inhibition effect of too high local concentration on microorganism metabolism is reduced through gradient labeling, and the accuracy of soil microorganism carbon utilization efficiency determination is improved. The method has the advantages that marking uniformity is improved, multiple groups of soil samples are arranged, real-time change of microbial respiration is captured by means of dynamic monitoring, influence of isotope fractionation on carbon dioxide concentration calculation is eliminated, instantaneous errors of traditional static measurement are avoided, and accuracy of analysis results is improved to a certain extent.

The invention discloses a land comprehensive improvement quality evaluation method and system based on big data, relates to the technical field of land resource management, and solves the problem of lack of effective quantification means for more critical ecosystem function indexes. The invention discloses a land comprehensive improvement quality evaluation method and system based on big data. The method comprises the steps that a remote sensing image module and an optical image module are used for collecting image data of soil surface vegetation; uploading the acquired image data to a cloud platform through a data network, and establishing a soil surface vegetation optical evaluation index database; the carbon dioxide monitoring module and the oxygen monitoring module are used for monitoring the carbon dioxide concentration and oxygen near the vegetation on the soil surface in real time. According to the method, a soil surface vegetation carbon sequestration evaluation index database is established to calculate vegetation net primary productivity, biomass and carbon utilization efficiency, the vegetation net primary productivity, biomass and carbon utilization efficiency are summarized through a software program, and an ecological quality comprehensive index of soil surface vegetation is given.

The invention discloses a method for quantifying response of two carbon utilization modes of microalgae to weathered carbon sink of magnesium-containing silicate rock, which comprises the following steps: culturing two groups of microalgae under the same culture condition, adding magnesium-containing silicate rock and microalgae into one group, and setting an inhibitor (AZ / DIDS) added with a certain carbon utilization mode as an experimental group; and only magnesium-containing silicate rock and microalgae are added into one group as a blank group without adding an inhibitor (AZ / DIDS) of a certain carbon utilization mode. Setting the treatment group added with AZ (10mM) and DIDS (2mM) as an experimental group, and subtracting the weathering quantity of the magnesium-containing silicate rocks in the treatment group not added with AZ / DIDS and the treatment group added with AZ / DIDS to obtain the weathering quantity delta Ti of the magnesium-containing silicate rocks under the influence of the microalgae anion channel utilization mode, namely delta Ti = Ti-Ti (AZ / DIDS), and rock weathering carbon sink Qi = delta Ti under the influence of the microalgae anion channel utilization mode. Fitting a polynomial function Q = Q (i) to obtain the response function of the microalgae extracellular carbonic anhydrase mode or the microalgae anion channel utilization mode to the weathering carbon sink of the magnesium-containing silicate rock. According to the method, the rock weathering carbon sink quantity under the influence of two carbon utilization modes of the microalgae can be rapidly and accurately obtained. The invention provides the method for quantifying the response of the two carbon utilization modes of the microalgae to the weathered carbon sink of the magnesium-containing silicate rock so as to overcome the defect that the response of the two carbon utilization modes of the microalgae to the weathered carbon sink of the magnesium-containing silicate rock cannot be quantified in the prior art.

The invention discloses a heavy oil thermal recovery technology carbon utilization efficiency evaluation method comprising the following steps: 1, data collection and pretreatment: collecting data of petroleum coke production, transportation, treatment and utilization links, and carrying out pretreatment and verification; 2, dynamic coupling modeling is conducted, specifically, a multi-physics field simultaneous model is constructed, and a reaction efficiency function is corrected in real time through reservoir numerical simulation; 3, carbon flow analysis: calculating input carbon flow, effective carbon utilization and lost carbon flow according to the carbon flow analysis matrix, and realizing real-time monitoring in combination with a digital twinborn system; 4, index calculation: calculating total carbon utilization efficiency, primary conversion efficiency and secondary cycle efficiency, and introducing carbon negative potential evaluation; 5, dynamic correction, wherein the total carbon utilization efficiency is dynamically corrected according to the petroleum coke quality coefficient, the system integration degree coefficient and the biomass blending combustion coefficient; and 6, evaluation reference comparison: comparing the calculated total carbon utilization efficiency with an evaluation reference value.

The present invention discloses a method for enhancing methane production and carbon sequestration in anaerobic digestion by zero-valent iron in cooperation with exogenous CO2. The method includes: inoculating sludge in a reactor, adding zero-valent iron thereto, and then adding wastewater. The reaction temperature is maintained at 35 ± 1 °C, and CO2 is injected into the liquid surface at a flow rate of 1.5 L / min for 5 - 50 min / L under stirring. CO2 and zero-valent iron jointly regulate the pH of the system, providing a suitable anaerobic digestion environment for microorganisms. Through the corrosion reaction of zero-valent iron under anaerobic conditions, electrons are released and H2 is generated, stimulating the proliferation of hydrogen-consuming microorganisms, while improving the activity of microorganisms and enzymes, increasing the methane production and rate. In addition, some methanogens in the system utilize H2 and CO2 to produce amorphous carbon, and zero-valent iron and exogenous CO2 are used to regulate the growth of amorphous carbon, strengthening the fixation of CO2 in the system.

The invention discloses a method for reducing an oxygen-enriched side-blown iron-containing material by using a biomass carbon source, and particularly relates to the technical field of metallurgical process optimization and efficient utilization of carbon resources. By constructing a multi-point temperature monitoring system and combining carbon source pretreatment, oxygen enrichment disturbance control and rhythm function driving, dynamic matching of carbon source release and an oxygen channel is achieved, an alternate local reduction window is formed, and the reduction efficiency and the carbon utilization rate are effectively improved; meanwhile, by recognizing a high-residual reducing gas area, adjusting a tail gas extraction path and guiding the tail gas into a preheating area, tail gas heat value recovery and carbon recycling are achieved; the method has the advantages of high reaction efficiency, low carbon emission, low energy consumption and high system controllability, and is suitable for low-carbon metallurgy and resource recycling scenes.

The invention discloses a tight reservoir CO2-WAG oil displacement and burying capacity calculation method, device and equipment, and the method comprises the steps: constructing a first seepage control equation representing three-phase seepage control of a matrix system and a second seepage control equation representing three-phase seepage control of an artificial fracture system according to the law of mass conservation and momentum conservation; establishing a three-phase seepage auxiliary equation; and solving the first seepage control equation, the second seepage control equation and the three-phase seepage auxiliary equation based on the basic parameters of the tight reservoir of the target area, and calculating to obtain the productivity data of carbon dioxide-water vapor alternate driving carbon utilization and burying of the tight reservoir. According to the method, the influence of factors such as diffusion mass transfer, three-phase capillary pressure, three-phase relative permeability hysteresis and fluid channeling between fractures and matrixes on the CO2-WAG flooding seepage law of the tight reservoir is fully considered, and accurate and effective prediction and evaluation of the productivity and carbon dioxide burying potential of the tight reservoir in the target area can be achieved.

The invention discloses a hydrogen-powered decoupling type carbon dioxide electrochemical reduction system and method, and belongs to the field of carbon dioxide electrochemical reduction, and the method comprises the following steps: under the driving of renewable electric energy, a carbon dioxide electrochemical reduction reaction is carried out in a cathode region of an electrolysis system, and carbon dioxide is reduced under the action of a catalyst; carrying out gas-liquid separation and gas drying to obtain gas and liquid products; pCET electrochemical oxidation reaction of an organic matter electric coupling agent is carried out in the anode region; an organic matter electric coupling agent is introduced as a medium, H2 oxidation is separated from an electrochemical process, and meanwhile, energy released by H2 oxidation is utilized to promote a low-energy-consumption carbon dioxide electroreduction process; the oxidized organic matter electric coupling agent is circulated to an external buffer tank through a pump, and a buffer tank solution is circulated to the reduction reaction tower through a pump and reacts with hydrogen in the reduction reaction tower to be chemically reduced to achieve regeneration. The electrolysis energy consumption is reduced, the carbon utilization rate is increased, and the method plays an important role in low-carbon comprehensive utilization.

An integrated process and system for the production of at least one gas fermentation product from a gaseous stream has been developed. The disclosure provides improved carbon utilization through both the recycle of a bioreactor tail gas via various different flow schemes and the employment of a CO2 to CO conversion system such as a reverse water gas shift unit and hydrogen separation unit. Recycling of the bioreactor tail gas and employment of a CO2 to CO conversion process provides for favourable H2:CO molar ratios of the feed to the gas fermentation bioreactor(s) for enhanced production of fermentation products.

The invention discloses a transportation path optimization method and system for carbon emission and wind, light and solid waste collaborative emission reduction, and the method comprises the steps: obtaining the type of a CO2 emission source according to the type, latitude and longitude and yield of an emission enterprise; acquiring the trapping cost according to the type of the emission source; according to the geographic information software in combination with the spatial geographic data, determining a transportation site type of the available construction pipeline, and according to the transportation site type, obtaining a transportation cost; according to the type and the spatial position of the geological sequestration place, obtaining sequestration cost and sequestration income; according to the wind and light place, determining the wind and light power generation potential to evaluate the potential and cost of hydrogen production by electrolysis of water, and obtaining the first carbon utilization cost and first carbon utilization income of methanol production by carbon dioxide hydrogenation; according to the type, latitude and longitude and yield of the solid waste, obtaining second carbon utilization cost and second carbon utilization benefit of utilizing carbon dioxide by the solid waste; and constructing a transportation optimization model, optimizing a transportation path by taking the minimization of the total cost as an optimization target, and realizing CO2 transportation and emission reduction.

The invention discloses a method for realizing carbon neutralization of a hydrogen-rich carbon circulating oxygen blast furnace, and relates to the technical field of metallurgical engineering, and the method comprises the following steps: deploying a gas analysis device and a sensor, and collecting multi-dimensional data of environmental gas in the blast furnace; establishing a carbon emission baseline model through a machine learning algorithm in combination with historical carbon emission data, and generating a real-time carbon emission trend prediction report; constructing an in-furnace multiphase collaborative reaction model, and calculating carbon utilization efficiency distribution and carbon emission source positioning according to a carbon emission trend prediction report; on the basis of carbon utilization efficiency distribution and carbon emission source positioning, the injection strategy of the hydrogen-rich gas is adjusted, and a dynamic injection plan is obtained; according to the dynamic injection plan, reaction process parameters of a solid phase and a liquid phase in the furnace are adjusted, and regionalized distribution of a thermal phase is regulated and controlled; through optimization of the machine learning algorithm, the multiphase collaborative reaction model and the dynamic injection strategy, the carbon utilization efficiency is improved, carbon emission is reduced, and the overall production efficiency and energy efficiency of the blast furnace are improved.

The invention discloses a method for detecting the carbon utilization efficiency of soil microorganisms based on fermentation bed padding, and particularly relates to the technical field of soil utilization efficiency detection.The method comprises the steps that a target fermentation bed padding sample and a corresponding applied soil sample are obtained; measuring a target fermentation bed padding sample and an applied soil sample, and constructing a characteristic parameter set; determining the microbial carbon utilization efficiency corresponding to the applied soil sample by adopting an isotope labeling method; training the input variables and the output variables by using a machine learning algorithm, and establishing a microbial carbon utilization efficiency prediction model; and predicting the carbon utilization efficiency of the to-be-detected target fermentation bed padding. The soil microorganism carbon utilization efficiency detection method is constructed through sample data acquisition, characteristic parameter determination, carbon utilization efficiency reference value determination, prediction model construction and training and carbon utilization efficiency prediction, and the problems of low detection efficiency, insufficient detection pertinence and accuracy, lack of an efficient prediction system and the like are solved.

The invention provides an oxamido multi-nitrogen-source core-shell structure long-acting slow release fertilizer as well as a preparation method and application thereof. The slow-release fertilizer is of a core-shell structure, the core-shell structure comprises an inner core area and a shell, the inner core area at least comprises a quick-acting core, and raw materials of the quick-acting core comprise a quick-acting nitrogen source, a medium trace element compound, humus, poly-polyhydroxy acid and a first auxiliary agent; the shell is prepared from the following raw materials: slow-release nitrogen source oxamide, a second auxiliary agent and a biological agent. Controllable slow release within 50-200 days is achieved, the nitrogen utilization rate is increased by 1.25-2 times compared with a conventional compound fertilizer, the nitrogen and carbon utilization rate is remarkably increased, and carbon emission to the atmosphere is reduced. The fertilizer can meet the requirements of the whole growth period after being applied once, is suitable for food crops and commercial crops, and has remarkable economic and environmental benefits.

The invention belongs to the field of biomass high-valued utilization, and provides a method and system for estimating the carbon utilization efficiency of forest biomass high-valued utilization, and the method comprises the steps: determining the initial carbon component content based on the forest type; converting the woods according to a biomass high-valued conversion process, and determining the content of carbon components in the woods after each step of process is completed; constructing a carbon transfer matrix of each step of flow according to the carbon component contents of the input raw materials and the output products of each step of flow; determining the final carbon component content after the forest biomass is subjected to high-valued treatment by utilizing the initial carbon component content and the carbon transfer matrix of all the processes; and determining the high-valued carbon utilization efficiency of the forest biomass based on the ratio of the final carbon component content to the initial carbon component content after the forest biomass is subjected to the high-valued treatment. According to the method, the accuracy of estimating the high-value utilization carbon utilization efficiency of the biomass can be improved, and a reliable measurement index is provided for the technical improvement of the high-value utilization of the biomass.

The application discloses a kind of determination methods of soil microbial carbon utilization efficiency, it is related to the determination field of carbon utilization efficiency, the application includes pre-culturing soil sample, multi-source 18 O Isotope gradient labeling, dynamic gas monitoring, dynamic correction of microbial respiration rate, microbial growth rate and carbon utilization efficiency acquisition, by multi-source isotope labeling, the contribution of different oxygen sources to microbial DNA synthesis is quantified, the low estimation of carbon utilization efficiency caused by traditional single labeling method is avoided, the limitation problem existing in the current soil microbial carbon utilization efficiency determination process is solved, gradient labeling reduces the inhibitory effect of local concentration on microbial metabolism, improves the uniformity of labeling, sets multiple soil samples, simultaneously uses dynamic monitoring to capture the real-time change of microbial respiration, eliminates the influence of isotope fractionation on carbon dioxide concentration calculation, avoids the instantaneous error of traditional static measurement, to a certain extent, the accuracy of analysis result is improved.

The invention discloses a distributed carbon management integrated system capable of automatically realizing carbon capture, carbon utilization and carbon sequestration in a single system. The distributed carbon management integrated system comprises a direct air capture unit, an electrochemical regeneration unit, a carbon dioxide release and pressurization unit, a water mineral separation and carbon sequestration unit, a circulating liquid transmission system and a control module. Carbon dioxide in the air is firstly adsorbed by the copper-chelated alkalescent resin, and the resin after adsorption saturation is regenerated through alkali liquor generated through electrochemistry and forms a sodium bicarbonate solution. The sodium bicarbonate solution enters a closed container filled with weakly acidic resin to be subjected to acidification reaction, carbon dioxide gas is generated through self-pressurization and conveyed to the water mineral separation and carbon sequestration unit under pressure driving, and calcium carbonate precipitates generated in the water are subjected to solid-state sequestration. According to the invention, integration of carbon capture, regeneration, conversion and sequestration and low-energy-consumption operation are realized, and the system is suitable for distributed carbon management application scenarios.

The present invention relates to a Carbon Capture Utilization and Storage (CCUS) opportunity through well rehabilitation. Aqua Freed® and Aqua Gard® are well-known well rehabilitation and preventive well maintenance technologies that utilize (inject into the subsurface in a methodical way) liquid CO2 for the purpose. The object of this invention was to quantify the storage capacity of Aqua Freed® and Aqua Gard® and establish their CCUS credentials.

The invention provides a method and system for detecting the carbon utilization rate of microorganisms based on a microwell plate respiratory system, and the method comprises the following steps: pre-culturing target microorganisms, and confirming the logarithmic growth phase of the target microorganisms; the method comprises the following steps: inoculating target microorganisms into a plurality of deep holes of a microporous plate respiratory system for culture, wherein each deep hole in the microporous plate respiratory system comprises a bacterial liquid containing a specified carbon source; detecting the initial biomass in the bacterial liquid and the initial carbon dioxide value in the microplate respiratory system at the beginning of culture, and detecting the final biomass in the bacterial liquid and the final carbon dioxide value in the microplate respiratory system at the end of culture; calculating the growth rate of the target microorganisms according to the initial biomass and the end biomass; calculating the respiration rate of the target microorganism according to the initial carbon dioxide value, the end carbon dioxide value and the growth rate; and calculating the carbon utilization rate of the target microorganism according to the growth rate and the respiration rate. The problem that the carbon utilization rate of species level microorganisms is difficult to obtain is solved.

The invention belongs to the technical field of microbial agents for soil improvement, and particularly relates to application of a microbial agent for promoting carbon immobilization of a red soil rice field. The microbial inoculum is rhodococcus erythropolis or a synthetic microbial inoculum of rhodococcus erythropolis and pseudomonas aeruginosa, and after the rice seedlings are transplanted to red soil for 3 days, 25mL of suspension liquid of the related microbial inoculum is inoculated to the rhizosphere of each rice seedling. According to the application, the rhizosphere microenvironment is improved, input of plant photosynthetic carbon into soil is activated, meanwhile, the carbon utilization efficiency and conversion capacity of soil in-situ microbial communities are remarkably improved, accumulation of microbial source carbon is promoted, the molecular composition structure of soluble organic matter is remodeled, and finally formation and accumulation of a soil carbon library are promoted and stabilized.

The application discloses a soil microbial carbon utilization efficiency detection method based on fermented bed litter, and particularly relates to the technical field of soil utilization efficiency detection, which comprises the following steps: obtaining target fermented bed litter samples and corresponding post-application soil samples; determining the target fermented bed litter samples and the post-application soil samples, and constructing a characteristic parameter set; determining the microbial carbon utilization efficiency of the post-application soil samples by using an isotope labeling method; training input variables and output variables by using a machine learning algorithm, establishing a microbial carbon utilization efficiency prediction model; and predicting the carbon utilization efficiency of the target fermented bed litter to be detected. Through sample data acquisition, characteristic parameter determination, carbon utilization efficiency benchmark value determination, prediction model construction and training, and carbon utilization efficiency prediction, the application constructs a soil microbial carbon utilization efficiency detection method, and solves problems such as low detection efficiency, insufficient detection pertinence and accuracy, and lack of an efficient prediction system.

The application discloses a kind of visual straw utilization characteristic analysis method and system, belong to soil carbon emission field, the method includes: collection soil physical and chemical property data and the characteristic data of different types of rotten straw, and the data collected are preprocessed, and integrated database is obtained by construction;The data in integrated database is processed by biological carbon utilization characteristic analysis model, simulates the dynamic process of soil carbon cycle after corresponding type of rotten straw is applied, obtains key variable data;According to the key variable data calculated, traverse the time series in integrated database, calculate carbon sequestration efficiency, microbial activity and physical protection intensity, obtain enhanced time series data, use enhanced time series data and key variable data as data source, and render to obtain visual diagram.The application improves the calculation accuracy and accuracy of soil carbon emission flux and accumulation.

The invention discloses a visual straw utilization characteristic analysis method and system, and belongs to the field of soil carbon emission.The method comprises the steps that soil physicochemical property data and characteristic data of different types of decomposed straw are collected, the collected data are preprocessed, and a comprehensive database is constructed; processing the data in the comprehensive database through the biological carbon by utilizing a characteristic analysis model, and simulating a soil carbon cycle dynamic process after applying the decomposed straws of the corresponding type to obtain key variable data; and traversing the time sequence in the comprehensive database according to the calculated key variable data, calculating carbon sequestration efficiency, microbial activity and physical protection intensity to obtain enhanced time sequence data, and rendering by taking the enhanced time sequence data and the key variable data as data sources to obtain a visualization graph. According to the method, the calculation precision and accuracy of the soil carbon emission flux and cumulant are improved.

According to the method for preparing methanol through biomass supercritical carbon dioxide gasification, supercritical carbon dioxide serves as a gasifying agent at high temperature, cracking of biomass macromolecules can be promoted, and generation of heavy tar is reduced. In the gasification stage, supercritical carbon dioxide reacts with carbon in biomass, and solid-phase carbon is converted into gas-phase CO, so that the carbon utilization rate is remarkably increased. Supercritical carbon dioxide is used as a green solvent to replace a traditional gasifying agent, nitrogen dilution or extra air separation energy consumption is avoided, meanwhile, part of CO2 is stored, carbon emission is reduced, and therefore the economical efficiency and environment friendliness of the whole process are improved.

According to the industry multi-energy complementary carbon neutralization system, a natural energy conversion system and a thermal power station output electric energy to an electric energy supply system, and the electric energy supply system supplies the electric energy; the thermal power station outputs carbon dioxide to the coal chemical system through the carbon reuse system; the coal chemical industry system outputs an energetic solid and an energetic liquid to the steel and iron manufacturing system; the carbon reuse system comprises a carbon capture system and a carbon utilization system, and the carbon capture system captures carbon dioxide and conveys the carbon dioxide to the carbon utilization system; the carbon utilization system receives carbon dioxide of the thermal power station and the carbon capture system and outputs the carbon dioxide to the coal chemical system. A multi-energy complementary carbon neutralization mode is achieved, renewable energy sources are brought into the high-energy-consumption industry, and low-carbon transformation of the renewable energy sources is accelerated; and moreover, the use ratio of fossil energy is reduced, the energy utilization efficiency is improved, carbon dioxide emission is reduced, the energy utilization ratio of new energy in the high-energy-consumption industry is improved, and low carbonization of the emission side in the high-energy-consumption industry is achieved.

This invention pertains to oil and gas development and carbon utilization, specifically relating to a process for synergistic methane production enhancement based on CO2 electrochemical reduction and microbial synthesis. The process first converts CO2 into a formic acid donor liquid in an electrochemical reduction system. Then, *Methanobacterium formic acid* is inoculated into an anaerobic culture medium with formic acid as the primary electron donor for activation and culture. Subsequently, the resulting formic acid donor liquid, methanogenic microbial activation liquid, Fe / Ni synergistic activation components, and nutrients are mixed to prepare a synergistic production enhancement injection fluid. This fluid is then injected into the target coal seam via staged fracturing and slug injection. After injection, the well is shut in for cultivation and production is resumed, with supplementary injections performed as needed. This invention organically combines CO2 resource conversion, microbial activation, coal seam injection, and sustained production enhancement, improving electron donor supply efficiency and methane generation efficiency, improving coal seam seepage conditions, enhancing sustained production enhancement in low-permeability coal seams, and expanding the resource utilization of CO2 in coalbed methane development.

This invention provides a uracil-producing strain, its construction method, and its application. The strain utilizes CRIPSR / Cas9 gene editing technology to produce... E. coli On the UR14 genome, firstly by knocking out psuG Gene, black Gene, rutA Genes and upp Genes were introduced to block the breakdown of uracil; then, the uridine phosphorylase gene was overexpressed. udp and pyrimidine-5'-nucleotide nuclease gene ppnN This synergistically enhances the synthesis and accumulation of uracil; finally, overexpression of the phosphoribosyltransferase gene... pgm, Further enhancing the conversion of the byproduct 1-phosphate ribose into the precursor 5-phosphate ribose-1-pyrophosphate improves carbon utilization. The resulting strain exhibits good genetic stability and high fermentation yield, enabling stable production of uracil and demonstrating broad application prospects.

Provided are systems and methods for converting C1 substrates to products contain more than one carbon, without producing central metabolic building blocks as intermediate products. In an embodiment, system / method can include a biochemical pathway enabling an orthogonal platform for C1 utilization based on formyl-CoA elongation (FORCE) reactions. In an embodiment, the system / method can include acyloin condensations between formyl-CoA and carbonyl-containing molecules. In an embodiment, the system / method can include a reactions catalyzed by the enzyme 2-hydroxyacyl-CoA lyase (HACL).

The present invention discloses a Bacillus licheniformis cell for fixing carbon dioxide, belonging to the field of fermentation engineering. In the present invention, two recombinant strains were constructed by overexpressing phosphoenolpyruvate carboxykinase and acetyl-CoA carboxylase in Bacillus licheniformis respectively. Under anaerobic fermentation conditions, compared with the control strain, the conversion rates of the products lactic acid, 2,3-butanediol, and acetic acid of the recombinant strain overexpressing phosphoenolpyruvate carboxykinase were increased by 19.6%, 34.7%, and 51.1% respectively; the conversion rates of the three products of the recombinant strain overexpressing acetyl-CoA carboxylase were increased by 22.9%, 14.8%, and 75% respectively. The recombinant strains constructed by the present invention have practical significance for reducing carbon dioxide emissions and increasing carbon utilization.