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Method for fast establishing DSE and plant symbiosis cultivation system and uses thereof

A symbiotic culture system, symbiotic system technology, applied in the direction of botany equipment and methods, applications, horticultural methods, etc., can solve the problems of DSE slow growth, low efficiency, easy to be contaminated by bacteria, etc., to achieve short establishment time and high efficiency Effect

Inactive Publication Date: 2008-09-17
YUNNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The common disadvantages of the above two methods are that they are easily contaminated by bacteria, DSE grows slowly, and the symbiotic culture system takes a long time to establish and has low efficiency.
[0004] Through document search, do not find the public report of the same document as the content of the present invention

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Embodiment 1: Phialophora sp. (Phialophora sp.) Symbiosis system cultivation of DSE-maize

[0019] 1. Phialophora sp. strain activation and cultivation

[0020] Four strains of Phyllophyllum Y10, H122b1, H112a, and H122b were activated from the slant of the frozen test tube, and then inoculated into plates containing PDA medium, and cultured at 28°C for 2 weeks in the dark. Use a sterile puncher to punch several bacterial pieces along the edge of the colony for later use.

[0021] 2. Cultivation of sterile corn seedlings

[0022] Use 75% ethanol 5min successively with corn seed, 10% sodium hypochlorite 10min surface disinfection, rinse 3 times with sterile water, each 3min, then inoculate in sucrose agar medium (sucrose 15g / L, agar 8g / L) plate, every Dish 5 capsules. Cultivate at a constant temperature of 25°C for 72 hours, and select seedlings with consistent growth without pollution for future use.

[0023] 3. Establishment of DSE and maize co-system

[0024] (1)...

Embodiment 2

[0033] Embodiment 2: Symbiosis experiment of exophiala (Exophila sp.) DSE and corn

[0034] The experimental strains are Exophiala sp. DSE preserved in the strain room of our laboratory, and the strain numbers are H50, H125, H114, and B3b. The specific experimental procedures are the same as in Example 1. Microscopy results showed that DSE mycelium was colonized in the corn roots under the inoculation treatments of H50, H125, H114, and B3b, and microsclerotia appeared in some roots, which indicated that the symbiotic system between the four strains of Exophila sp. and corn was successfully established. No DSE colonization was found in the blank control.

Embodiment 3

[0035] Example 3: Preliminary detection of suspected DSE strains

[0036] The experimental strains are four suspected DSE strains isolated by our laboratory, whose taxonomic status has not been clarified, and their numbers are H49, L45b, H86, and H882c. The experimental procedure is the same as in Example 1. Microscopic examination results showed that DSE mycelium was colonized in the corn roots of L45b, H86, and H88-2c inoculation treatments, and microsclerotia appeared in some roots. No typical DSE structures such as microsclerotia and intracellular mycelium were found in the corn roots treated with H49 inoculation, and mycelium radial mycelia clusters appeared in some root sections, which were identified as Chaetomium sp. fungi by morphological identification .

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Abstract

The invention relates to a method for the fast establishment of DSE and plant symbiotic culture system and the application of the method, in particular to a method for the fast establishment of dark septate endophytes and plant symbiotic culture system and the application of the method, belonging to the field of applied microbiology, plant nutrition and ecology. The invention is characterized in that, based on the biological characteristics of DSE of wide carbon utilization and aerobe, riversand with good air permeability is used as the solid substrate; improved MS culture medium is added as the nutrient solution, and early nutritional growth requirements for the formation of DSE-plant symbiotic system are satisfied; the invention utilizes plant seedlings and DSE as materials; by inoculating DSE and plant seedlings on an aseptic culture medium, symbiotic relationship is formed. The method of the invention has the advantages of short setup time of the DSE and plant symbiotic culture system, high efficiency and other excellences; the invention can be applied to the study of the relationship between DSE and plants, the study of DSE morphology, the initial detection of suspected DSE bacterial strains and the application of DSE inoculation.

Description

Technical field: [0001] The present invention relates to a method and application thereof for quickly establishing a symbiotic culture system between DSE and plants, specifically a method for rapidly establishing a symbiotic culture system for dark-colored compartmentalized endophytic fungi and plants and its application, belonging to applied microbiology, plant nutrition and ecology field of study. Background technique: [0002] Dark septate endophytes (Dark Septate Endophytes, DSE) is not a scientific taxonomic unit, but generally refers to a group of small soil fungi that settle in plant roots. There are obvious transverse septa, which widely exist in the cells or intercellular spaces of healthy plant root epidermis, cortex, and even vascular tissue, but the pathological characteristics caused by pathogenic bacteria will not form in the root tissue. It has been reported that more than 600 species of plants have DSE colonization in their roots, and they are distributed in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G31/00A01G7/00A01C1/08C12N1/14
Inventor 刘茂军李涛赵之伟
Owner YUNNAN UNIV
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