Materials and methods for enhanced carbon utilization and/or sequestration as well as reducing deleterious atmospheric gases

A technology of utilization and carbon sequestration, which is applied in the removal of solid waste, chemical instruments and methods, and botanical equipment and methods. Toxic cost-effective effect

Pending Publication Date: 2021-05-25
LOCUS IP CO LLC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In agriculture, excessive application of nitrogen-containing fertilizers and poor soil management practices also contribute to increased nitrous oxide emissions

Method used

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  • Materials and methods for enhanced carbon utilization and/or sequestration as well as reducing deleterious atmospheric gases
  • Materials and methods for enhanced carbon utilization and/or sequestration as well as reducing deleterious atmospheric gases
  • Materials and methods for enhanced carbon utilization and/or sequestration as well as reducing deleterious atmospheric gases

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0210] Preparation of Microbial-Based Products

[0211] A microorganism-based product of the present invention is simply the fermentation medium containing the microorganisms and / or microbial metabolites and / or any residual nutrients produced by the microorganisms. Fermented products are used directly without extraction or purification. Extraction and purification can be readily achieved, if desired, using standard extraction and / or purification methods or techniques described in the literature.

[0212] Microorganisms in microbial-based products may be in active or inactive form, or in the form of vegetative cells, germ spores, mycelium, hyphae, conidia or any other form of microbial propagules. Microorganism-based products may also contain combinations of these forms of microorganisms.

[0213] In one embodiment, different microbial strains are cultured separately and then mixed together to produce a microbial-based product. Optionally, the microorganism may be mixed with...

Embodiment 1

[0241] Example 1 - Compositions based on microbial products

[0242] Illustrated herein are compositions for reducing greenhouse gases, increasing carbon utilization, and / or enhancing carbon sequestration, according to certain embodiments of the invention. This example is not intended to be limiting. Formulations comprising other types of microorganisms which replace or supplement those exemplified herein may be included in the composition.

[0243] The composition comprises a microbial inoculant comprising Trichoderma. Fungi and Bacillus bacteria. In a particular example, the composition comprises Trichoderma harzianum and Bacillus amyloliquefaciens. More specifically, the strain of Bacillus amyloliquefaciens may be Bacillus amyloliquefaciens subsp. viridans.

[0244] In one embodiment, the composition may comprise 1 to 99% by weight Trichoderma and 99 to 1% by weight Bacillus. In some embodiments, the ratio of the number of Trichoderma to Bacillus cells is about 1:9 to ...

Embodiment 2

[0260] Example 2 - Increase in Underground Biomass (Root Mass) of Citrus Trees

[0261] The composition comprising Trichoderma harzianum and Bacillus amyloliquefaciens was applied three times every two months to the soil with orange and grapefruit trees. Before and after treatment, root mass was measured and compared to untreated control trees ("growers' practice").

[0262] Such as Figures 1A-1B As shown, there was a significant difference in fibrous root biomass between untreated control and treated trees.

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PUM

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Abstract

The subject invention provides materials and methods for reducing deleterious atmospheric gases, such as greenhouse gases. In specific embodiments, the reduction in deleterious atmospheric gases is achieved via enhanced vegetative carbon utilization and storage, as well as increased carbon sequestration in soil. In some embodiments, the subject invention can be used for reducing the number of carbon credits used by an operator involved in, e.g., agriculture, livestock production, waste management or other industries. In certain embodiments, the subject invention provides customizable microbe-based products, as well as methods of using these microbe-based products for reduction of greenhouse gases and / or enhanced sequestration of carbon.

Description

[0001] Cross References to Related Applications [0002] This application claims priority to U.S. Provisional Patent Application No. 62 / 743,354, filed October 9, 2018, and U.S. Provisional Patent Application No. 62 / 884,720, filed August 9, 2019, which Both are incorporated herein by reference in their entirety. Background technique [0003] Gases that trap heat in the atmosphere are called "greenhouse gases" or "GHGs," and include carbon dioxide, methane, nitrous oxide, and fluorinated gases (U.S. Climate Change Indicators, 2016, Fourth Edition, U.S. Environmental Protection Agency, p. 6, hereinafter referred to as "2016 EPA Report"). [0004] carbon dioxide (CO 2 ) into the atmosphere through the burning of fossil fuels (coal, natural gas, and oil), solid waste, trees, and wood products, and certain chemical reactions (eg, cement manufacturing). Plants remove carbon dioxide from the atmosphere by, for example, uptake as part of the biological carbon cycle. [0005] Methan...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/38A01N63/22A01N63/10A01N63/30A01N25/30C09K17/00
CPCA01N63/22A01N63/38A01N65/03A01N63/32A01N61/00A01N65/00C05F11/00B09C1/00C09K17/14Y02P60/22B09B1/00B09C1/105B09C2101/00Y02P60/21Y02W30/40Y02T50/678A01P15/00A01N25/04C05F11/02C05F11/08C05F11/10C05G5/20A01N63/00A01N25/30C09K17/00C12Y301/03C12Y302/01A01G22/22A01N63/27A01N63/20A01C21/00B01D53/62B01D53/84B01D2251/95B01D2255/804B01D2257/504B01D2258/06B09C1/10
Inventor 肖恩·法默肯·阿里贝克保罗·S·左恩卡蒂克·N·卡拉瑟尔基思·海德考恩
Owner LOCUS IP CO LLC
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