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A Dunaliella culture medium utilizing sodium bicarbonate as a carbon source and its application

A Dunaliella culture medium and sodium bicarbonate technology, applied in the direction of single-cell algae, microorganism-based methods, microorganisms, etc., to achieve the effect of avoiding the complexity of the ventilation system, avoiding the problem of high energy consumption, and good pH buffering effect

Active Publication Date: 2021-11-19
DALIAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, when bicarbonate is used as inorganic carbon, careful control of the calcium and magnesium ion concentrations in the initial medium is required, parameters that cannot be provided by existing technologies

Method used

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  • A Dunaliella culture medium utilizing sodium bicarbonate as a carbon source and its application
  • A Dunaliella culture medium utilizing sodium bicarbonate as a carbon source and its application
  • A Dunaliella culture medium utilizing sodium bicarbonate as a carbon source and its application

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Experimental program
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Effect test

Embodiment 1

[0036] Embodiment 1 The cultivation of Dunaliella salina and the addition of different concentrations of NaHCO 3 Effects on the pH of Dunaliella salina

[0037] Configure to add different concentrations of NaHCO 3 artificial seawater culture medium, the concentration of which was set to 25, 50, 100, 200, 300, 500mmol / L respectively, and Dunaliella salina was prepared according to 20*10 4 The density of cell / mL was inoculated in artificial seawater medium, and Dunaliella salina was cultured under the conditions of 25°C, light intensity of 10000Lux, LED light source, and light time of 12h / day to detect different concentrations of NaHCO 3 Effects of pH on the growth of Dunaliella salina.

[0038] Changes in medium pH were detected daily, such as figure 1 As shown, through the result analysis, NaHCO 3 When the added concentration was 500mmol / L, the pH in Salina culture medium was the lowest at 9.7 on the 7th day; NaHCO 3 Under the condition of adding concentration of 25mmol / L...

Embodiment 2

[0039] Embodiment 2 adds different concentrations of NaHCO 3 Effects on Cell Biomass and β-Carotene Accumulation of Dunaliella salina

[0040] Configure to add different concentrations of NaHCO 3 artificial seawater culture medium, the concentration of which was set to 25, 50, 100, 200, 300, 500mmol / L respectively, and Dunaliella salina was prepared according to 20*10 4 The density of cell / mL was inoculated in artificial seawater medium, and Dunaliella salina was cultured under the conditions of 25°C, light intensity of 10000Lux, LED light source, and light time of 12h / day to detect different concentrations of NaHCO 3 Effects on biomass accumulation of Dunaliella salina.

[0041] Changes in the number of algae cells and dry weight were detected every day, such as figure 2 As shown, through the result analysis, NaHCO 3 When the added concentration is 200mmol / L, the cell number of Salina cells is the highest on the 7th day, which is 1.5 million cells / ml; image 3 Shown, Na...

Embodiment 3

[0043] Embodiment 3 adds different concentration Ca 2+ , Mg 2+ Effects on Dunaliella salina biomass and β-carotene accumulation

[0044] Configured to add different concentrations of Ca 2+ , Mg 2+ Inoculate Dunaliella salina in the artificial seawater medium at a density of 200,000 cells / mL at 25°C, with a light intensity of 10,000Lux, an LED light source, and a light time of 12h / day. Dunaliella salina was cultured to detect different concentrations of Ca 2+ , Mg 2+ Effects on β-carotene accumulation in Dunaliella salina biomass.

[0045] Changes in the number of algae cells and dry weight were detected every day, such as Figure 4 , shown in 5, after the result analysis, Ca 2+ , Mg 2+ The higher the concentration, the higher the number of algae cells and the highest dry weight of cells, but the difference is not significant, the results show that Ca 2+ When the concentration is 3.0mmol / L, Mg 2+ When the concentration was 5.0mmol / L, Dunaliella salina accumulated the ...

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Abstract

The invention discloses a Dunaliella culture medium using sodium bicarbonate as a carbon source and its application, specifically a Dunaliella culture medium using bicarbonate as the sole carbon source, a method for cultivating Dunaliella using it, and optimization of the culture medium Including calcium and magnesium ion optimization, trace element optimization. Adopting the Dunaliella culture medium of the present invention can well provide the carbon source source used in Dunaliella, thereby ensuring the growth of Dunaliella. At the same time, using sodium bicarbonate as a carbon source can also increase the concentration of β-carrot in Dunaliella salina. More importantly, the use of bicarbonate as a carbon source can also maintain the pH in the culture system in a range that is conducive to the growth of Dunaliella salina, so as to improve the growth rate of Dunaliella salina, but not As for the death of the cultivated Dunaliella salina caused by the high pH, ​​the cultivation method is simple, low in cost and high in carbon utilization efficiency, and is suitable for industrial application.

Description

technical field [0001] The invention relates to the field of microalgae biotechnology, in particular to a Dunaliella culture medium using sodium bicarbonate as a carbon source, a method for culturing microalgae, and the use of microalgae to induce β-carotene. Background technique [0002] Dunaliella salina, also known as Dunaliella salina, is a salt-tolerant eukaryotic green alga that, under environmental stress conditions (high light intensity, temperature stress, nutritional stress, and heavy metal stimulation), forms lipid globules in the chloroplasts of cells. A large amount of β-carotene is accumulated in the body, which makes the whole cell appear orange-red, so Dunaliella salina is considered to be a model organism for studying the stress-resistant growth of organisms and carotenoid synthesis and metabolism. β-carotene is a natural antioxidant, mainly used in nutrition, medicine, cosmetics and food, etc. Since the 1980s, Dunaliella salina has been used in many countri...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/12C12P23/00C12R1/89
CPCC12N1/12C12P23/00
Inventor 迟占有席一梅
Owner DALIAN UNIV OF TECH
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