High effective tissue culture regeneration of cucumber, and method for transplanting of said seedlings with high viability
A technology for regenerating seedlings and cucumbers, applied in the biological field, can solve the problems of shortened internodes of regenerated cucumber plants, aging regenerated seedlings, difficulty in transplanting and survival, etc., achieve normal development, vigorous growth, and solve the effects of short internodes
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Embodiment 1
[0026] Select the parental seeds Tm of the South China variety "Green Angel" with full grains and uniform size, soak them in tap water for 30 minutes, first treat them with 75% ethanol for 30 seconds, then sterilize the surface with 2% NaClO for 10 minutes, and wash them three times with sterile water , dispersed on water-soaked absorbent cotton / filter paper. After being treated in the dark at 30°C for one day, they were cultured in a cultivation room at 25°C, with a photoperiod of 12 hr / day and a light intensity of 1500 lux to obtain sterile seedlings.
[0027] Take the cotyledons of sterile cucumber seedlings 2 days after "Lubai", cut off 1 / 2 of the cotyledons near the petiole end, and connect them with MS+6-BA 1mg / L+ABA 1mg / L+AgNO with the surface facing up 3 0.5mg / L+Ades 20mg / L+3% sucrose+0.7% agar bud induction and differentiation medium; then the explant material was placed in the culture room under the same conditions for culture, and after 12 days a large number of Cl...
Embodiment 2
[0031] Select the seeds 007-71321 of the North China type strain "Xintai Mici" with full grain and uniform size, soak them in tap water for 30 minutes, then treat them with 75% ethanol for 30 seconds, and then sterilize them with 2% NaClO for 10 minutes. Wash 3 times with water and spread on water-soaked absorbent cotton / filter paper. After being treated in the dark at 30°C for one day, they were cultured in a cultivation room at 25°C, with a photoperiod of 12 hr / day and a light intensity of 1500 lux to obtain sterile seedlings.
[0032] Take the cotyledons of sterile cucumber seedlings 2 days after "Lubai", cut off 2 / 3 of the cotyledons near the petiole end, and connect them with MS+6-BA 1mg / L+ABA 1mg / L+AgNO 3 1mg / L+Ades 20mg / L+3% sucrose+0.7% agar on the bud induction and differentiation medium; then put the explant material in the culture room under the same conditions for culture, and after 15 days, a large number of clusters can be seen on the edge of the cut bud. At th...
Embodiment 3
[0036] Select the parental seeds HL7 of the European and American strain "Little Angel 2" with plump grains and the same size, soak them in tap water for 30 minutes, then treat them with 75% ethanol for 30 seconds, then disinfect the surface with 2% NaClO for 10 minutes, and wash them with sterile water for 3 minutes. Once, spread on water-soaked absorbent cotton / filter paper. After being treated in the dark at 30°C for one day, they were cultured in a cultivation room at 25°C, with a photoperiod of 12 hr / day and a light intensity of 1500 lux to obtain sterile seedlings.
[0037] Take the cotyledons of sterile cucumber seedlings 2 days after "Lubai", cut off 1 / 2 of the cotyledons near the petiole end, and connect them with MS+6-BA 1mg / L+ABA1mg / L+Ades 20mg / L+3% Sucrose + 0.7% agar on shoot induction and differentiation medium; then the explant material was cultured in the culture room under the same conditions, and after 12 days, a large number of clustered shoots could be seen...
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