40results about How to "Increase fermentation intensity" patented technology

A method for producing L-lactic acid by Bacillus coagulans CGMCC No.2602 belongs to the technical field of microorganisms. In the invention, Bacillus coagulans CGMCC No.2602 is adopted, and under the condition of no oxygen supply, starchiness hydrolyzed sugar or dextrose is fermented by a semicontinuous intermittent fermentation way or an inter-sugar-compensating fermentation way to generate L-lactic acid with high optical purity. The invention has the advantages that the gemma property of the Bacillus coagulans is stable, and the L-lactic acid obtained by inoculating and fermenting the starchiness hydrolyzed sugar has high optical purity and rate of conversion of sugar and acid and short fermentation period; in addition, the semicontinuous intermittent fermentation way saves the time for preparing seeds by a continuous reladling and subculturing method, shortens the fermentation period, enhances the fermentation strength and obtains the L-lactic acid with both relatively high rate of conversion of sugar and acid and purity.

The invention relates to a process for producing acetone and butanol by biomass fermentation and pervaporation membrane coupling, in particular to a production process of coupling a pervaporation membrane and ABE (acetone, butanol and ethanol) fermentation, separating ABE in situ on line and utilizing an extraction tower to obtain an anhydrous solvent. Fermentation liquor in a fermentation tank is pumped out, and is filtered by an ethanol-permselective membrane to obtain a concentrated solution, the concentrated solution is sent into the extraction tower and is separated into an organic phase and a water phase in the extraction tower, after the water phase is pumped into a water phase storage tank, the organic phase does not pass a mash tower but directly enters a butanol tower and follow-up rectifying tower groups to obtain anhydrous butanol, acetone and ethanol, the water phase in the water phase storage tank passes a pervaporation ethanol-permselective membrane assembly, dialysate is pumped into the extraction tower, and the cycle is repeated. According to the process disclosed by the invention, the fermentation process is coupled with the pervaporation technology, the extraction tower is added behind the pervaporation assembly, the inhibitory action of the butanol in the fermentation process is reduced, energy consumption in the separation process is saved, and the competitiveness of a biomass fermentation method for producing the butanol is improved.

The invention relates to a method for preparing and regenerating a rhizopus oryzae protoplast for producing L-lactic acid at high yield, comprising the following steps of: A. preparing a rhizopus oryzae spore suspension; B. preparing a seed culture liquid; C. preparing a hypha suspension; D. enzymolyzing a cell wall; E. separating a protoplast; and F. regenerating the protoplast. The rhizopus oryzae protoplast prepared with the method disclosed by the invention is in an approximately-circular shape and has higher activity; the preparation quantity and the regeneration rate of the rhizopus oryzae protoplast can respectively reach 2.74*10<6>/mL and 6.8 percent to the maximum extent. The method is easy to operate, has low requirements for the equipment, and provides a technical reference for preparing and regenerating the rhizopus oryzae protoplast. By using the method disclosed by the invention, the protoplast can be quickly prepared with short regeneration period and high regeneration speed; and the preparation efficiency of the protoplast can be effectively improved. The protoplast prepared with the method provided by the invention completely meets the requirement for fusing the protoplast. The invention provides an operation method with practical significance for preparing and regenerating the protoplast.

The invention provides a critic acid production fermentation technology, which is characterized in that: during a critic acid fermentation process, protease is added into a fermentation tank through a fed-batch method so as to hydrolyze the protein in a culture medium into nitrogen sources, which can be rapidly utilized by bacterial strains, such as small molecular peptides, amino acid, and the like. The method can increases the content of amino acid, which can be directly utilized by bacterial strains, in a fermentation fluid; also can fully dissolves out the starch particles, which combine with the protein, reduces the viscosity of the fermentation fluid, improves the oxygen transmission efficiency in the fermentation fluid, promotes the bacterial strains to produce acid, solves the problem that in the current critic acid production process the protein utilization effect is bad in the early fermentation period, shortens the fermentation period, reduces the residual total sugar and grain consumption for fermentation, and increases the economic profits for entrepreneurs.

The invention discloses a novel technique for producing high optical purity L-lactic acid through rhizopus oryzae high cell density fermentation by a semi-continuous way, which relates to the following steps: (1) preparing rhizopus oryzae spore suspension; (2) preparing seed culture; (3) high-density thalli amplification; (4) 500L fermentation cylinder for the first batch fermentation; (5) 500L fermentation cylinder for high-density fermentation by the semi-continuous way-the first 5 times thalli proliferation and repeat fermentation; and (6) the last 20 times 500L fermentation cylinder for high-density and high-intensity repeat fermentation. The technique realizes semi-continuous fermentation by repeatedly using rhizopus oryzae thallis and increases the use ratio of raw material; the thallis are constructed in high density so that the fermentation period is shortened to be 18h and the fermentation intensity is increased to be 5.0g/(h*L), thus the technique has good industrial production prospect; the optical purity of the fermentation product of L-lactic acid is over 99.5 percent so as to satisfy the demands of foods and medicaments; and the existing demand for high-purity L-lactic acid is relieved to a great extent after industrial production.

The invention discloses a method for seed culture of Aspergillus niger and preparing citric acid. The method comprises the following steps: (1) Aspergillus niger mouldy bran spores which are cultivated and maturated, in order to obtain dispersed spore suspension; (2) dispersed spore suspension in the step (1) is cultivated in order to obtain germinated spores; (3) germinated spores are dispersed again, the spores are cultivated in a seed tank, in order to obtain matured seed liquid; (5) matured seed liquid is converted to a fermentation tank for cultivation and fermentation, in order to obtaincitric acid fermentation broth. The method solves the aggregation problems in spore germination process, in order to improve conglomeration rate of spores, increase quantity of mycelium pellets, effectively control uniformity of size of mycelium pellets, improve conversion efficiency of thallus, improve conversion rate of fermentation, and shorten fermentation period.

The invention provides a method for preparing citric acid by fermentation. The method comprises the following steps of: under the condition that the citric acid can be generated, inoculating aspergillus niger to a fermentation medium for fermentation, wherein the citric acid generated during fermentation is separated, and the concentration of the citric acid in fermentation liquor is enabled to be not higher than 13.5% by weight during fermentation and at a fermentation terminal point. According to the method, preferably, the citric acid generated during fermentation is separated by means of adopting an anion exchange adsorption method, so that the yield, fermentation strength and fermentation conversion rate of the citric acid are effectively increased, and the fermentation period is shortened simultaneously.

The invention discloses a cell cultivation method of immobilized rhizopus oryzae without a carrier. The steps are as that: (1) spores are inoculated with high concentration and then thick spores-suspended emulsion is formed. (2). a number of bacterium balls are produced by controlling the aeration condition. The proportion of sodium ion and potassium ion in the culture medium is controlled to lead the rhizopus oryzae spawn to increase and keep the stability of the cell form. Methyl silicone oil is added to form a plurality of bacterium ball cells with certain diameter. The bacterium balls cut flow and the immobilized rhizopus oryzae without a carrier are obtained. (3) when the bacterium ball obtained is transferred to a big pot, the upgrade of the bacterium balls and the increasing of the number are controlled. The invention has the advantages that firstly, the appearance of bacterium ball formed in the suspending culture system is regular and the size is equal; secondly, the condition of bacterium ball accumulated by cells is mild and controllable; thirdly, the density of bacterium ball cells is more than 10⁴/L and the cell activity is more than 95 percent; fourthly, the large-scaled culture is easy to realize; fifthly, the cost of immobilized carrier can be saved.

The invention discloses a method for coproduction of alcohol, fructose and multiple side products by using sweet sorghum stalks and corn. The method comprises the following process steps: (1) pretreatment of the sweet sorghum stalks; (2) pretreatment of the corn; (3) preparation of fructose syrup and the fructose; (4) preparation of fuel alcohol; (5) preparation of DDGS (Distillers Dried Grains with Solubles) and liquefied natural gas; (6) preparation of silage feed; (7) recovery of carbon dioxide. The method disclosed by the invention has the beneficial effects that by utilization of the sweet sorghum stalks and the corn as raw materials, all products can be ensured to have more stable raw-material sources, coproduction of multiple products such as the alcohol, the fructose, the liquefiednatural gas and the feed can be realized, the energy consumption of final products can be reduced, the comprehensive cost of the raw materials is low, the utilization is high, and the method is energy-saving and environmentally friendly and plays an important role in fully utilizing and transformation of saline-alkali wastelands in the northeast region of China.

The invention relates to the technical field of tartaric acid preparation and especially relates to a technology for preparing L(+)-tartaric acid through a coupling process. The technology comprises the following steps: preparing cis-epoxy calcium succinate by taking maleic anhydride as a raw material, reacting with calcium carbonate under the effect of a tungsten-containing catalyst and oxidizingwith hydrogen peroxide; preparing sodium tartrate by taking immobilized cells as a biocatalyst and enzymatically converting disodium cis-epoxysuccinate into sodium tartrate; coupling replacement: heating a sodium tartrate solution and then mixing with cis-epoxy calcium succinate, measuring the content of tartaric acid in supernate, and filtering when the content of tartaric acid does not declineany more, thereby acquiring solid calcium tartrate and filtrate; acidizing by adding sulfuric acid into the calcium tartrate, thereby acquiring L(+)-tartaric acid. According to the invention, the problem of high fermentation strength in the traditional preparation process is solved by using the immobilized cells and adopting the mode of coupling replacement, and the technology is water-saving andenvironment-friendly and is high in production benefit.

A clean process for preparing alcohol from wheat includes such steps as grinding, separating bran, adding water to wheat flour, separating out gluten powder, liquefying the suspension of residual starch, saccharifying, fermenting, rectifying to obtain 95% alcohol, dewatering to obtain absolute alcohol, concentrating and drying residue to obtain feed, aerobically treating the residual water and condensed water for reuse.

The ivneniton discloses a production process of fructo-oligosaccharide-neokestose. Immobilized phaffia rhodozyma cells are used repeatedly, continuous and high-intensity production of fructo-oligosaccharide-neokestose is performed through phaffia rhodozyma cells immobilized with an embedding method, the production cycle is shortened, the yield of neokestose is increased, the production cost is reduced, industrial and large-scale production of fructo-oligosaccharide-neokestose is successful, and the production process has excellent application prospect.

The invention relates to an orange and apple compound wine fermenting method. The method comprises the steps of cutting up cleaned apples, wherein the size of the cut apples is 0.3-0.5 cm<3> appropriately; adopting a 0.2% ascorbic acid solution for soaking, protecting the color, meanwhile, removing apple cores and seeds, extracting fruit juice, and obtaining original apple juice through filteringand clarification; peeling off orange peel, removing capsule dressing and seeds, extracting fruit juice, and obtaining original orange juice through filtering and clarification; blending the originalapple juice and the original orange juice according to the ratio being 1:(1-2), and obtaining an orange and apple compound solution; uniformly adding the orange and apple compound solution into a fermentation tank for sufficient stirring, and meanwhile uniformly and continuously adding a proper amount of brewing active dry yeast, and obtaining juice to be fermented; the fermentation cost is lowered, and meanwhile the fermentation strength is enhanced.

The invention discloses a preparation method of a seed pumpkin leaf feed. The preparation method comprises the following steps: cutting seed pumpkin leaves to 1-3 cm in length, and sequentially spraying formic acid and molasses on the surfaces of the seed pumpkin leaves, wherein the ratio of the volume of the finally used formic acid to the mass of the seed pumpkin leaves is 1-2 mL/kg, and the ratio of the mass of the finally used molasses to the mass of the seed pumpkin leaves is 20 g/kg; and then carrying out anaerobic fermentation on the seed pumpkin leaves. By using the method disclosed by the invention, the pH value of the seed pumpkin leaf feed is reduced, the contents of lactic acid and acetic acid are increased, the content of butyric acid is reduced, the content of soluble sugar is increased, and the dry matter loss and the ammoniacal nitrogen content are reduced; and the crude protein content and the peptide nitrogen ratio in the seed pumpkin leaf feed are increased, the fiber content is reduced, the fermentation quality of the seed pumpkin leaf feed is improved, the nutrient loss is reduced, the nutritional value is increased, and the seed pumpkin leaf feed has great application potential.

The invention discloses a plant cell screening device and a method for screening a synchronized plant cell line, and relates to the field of cell culture equipment. The plant cell screening device isused in cooperation with a ventilation device and comprises a tank body, a material supplementing unit and a waste liquid collecting unit, wherein a horizontal screen is arranged in the tank body anddivides the inner cavity of the tank body into a first cavity and a second cavity which communicate with each other; the first cavity is located above the second cavity; an inoculation opening, an airoutlet and a culture medium connector are arranged on the first cavity; the material supplementing unit and the waste liquid collecting unit communicate with the bottom part of the second cavity; andthe first cavity and the second cavity communicate with the ventilation device. By adoption of the plant cell screening device for screening the synchronized plant cell line, short screening period and high sterility degree are achieved; an obtained cell line has the characteristics of high homogeneity and synchronization degree; the culture period of mass production can be easily shortened; regulation and control of secondary metabolites of mass production can be easily realized; the abundance and stability of metabolites are improved; the fermentation strength is improved; and the production cost is reduced.