A kind of aspergillus niger seed culture and its method for preparing citric acid

A technology of Aspergillus niger and citric acid, which is applied in the field of microbial fermentation, can solve the problems of uneven size of mycelium balls, lower cell transformation efficiency, lower utilization rate of seed tanks, etc., so as to shorten the fermentation period, improve mass transfer and dissolved oxygen , the effect of preventing spore aggregation

Active Publication Date: 2020-06-05
JIANGSU GUOXIN UNION ENERGY CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the traditional seed cultivation process, spore germination and hyphae growth are all in the seed tank, and the cultivation time of the seed tank is increased by 1 / 3, which reduces the utilization rate of the seed tank and increases the energy consumption in the early stage
[0005] In addition, due to the charge adsorption between spores, the dispersed spores are easy to re-aggregate into clusters. After clustering, multiple spores can only form one mycelial ball, resulting in a decrease in the proportion of spores forming mycelial balls. The ball rate is only 60%; and the size of the mycelium balls is not uniform, some of the mycelium balls are too large in diameter, and a dense area is formed in the center, which cannot effectively contact with nutrients and oxygen, resulting in a decrease in the conversion efficiency of the bacteria

Method used

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  • A kind of aspergillus niger seed culture and its method for preparing citric acid
  • A kind of aspergillus niger seed culture and its method for preparing citric acid
  • A kind of aspergillus niger seed culture and its method for preparing citric acid

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Experimental program
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Effect test

Embodiment 1

[0031] Raw material processing: After crushing the corn, pass through a 60-mesh sieve; mix the obtained corn flour and tap water in a mass ratio of 1:3, and use Ca(OH) 2 Adjust the pH of the slurry to 6.0, and add Novozymes α-high temperature amylase according to the addition amount of 20U / g corn flour; the obtained slurry is first sprayed at 97°C, kept for 1.5h, then sprayed at 127°C, and flashed The temperature of the feed liquid is lowered to 95°C, and the temperature is kept for 1 hour. After the iodine test turns light brown, the qualified corn liquefaction liquid is obtained; 80% of the corn liquefaction liquid is filtered through a plate frame to remove the filter residue, and the sugar liquid is obtained.

[0032] Spore culture: Take the cultured mature spores of Aspergillus niger bran, add 5 times the volume of sterile water, shake and soak for 0.5h, filter with four layers of gauze to obtain a dispersed spore suspension, and filter the corn liquefaction solution throu...

Embodiment 2

[0036] Raw material processing: same as embodiment 1.

[0037] Spore culture: Take the cultured mature spores of Aspergillus niger, add 10 times the volume of sterile water, shake and soak for 12 hours, and filter with four layers of gauze to obtain a dispersed spore suspension. Dilute the sugar solution obtained by filtering the corn liquefaction solution with plate and frame with water to a total sugar concentration of 20 g / L; add starch residue to make the solid concentration reach 100 g / L; add 0.1% (v / v) volume Tween 80; Adjust the pH to 6.0; sterilize at 121°C for 15 minutes, cool to 39°C; insert the spore suspension so that the final concentration of spores is 6*10 9 cells / mL, cultivated at a temperature of 42° C. and a stirring speed of 100 rpm for 4 h, then increased the stirring speed to 1000 rpm, and stirred for 2 h to obtain a spore suspension.

[0038]Seed culture: The seed medium is prepared with corn liquefaction solution and ammonium sulfate, the total sugar co...

Embodiment 3

[0041] Raw material processing: same as embodiment 1.

[0042] Spore culture: take the mature Aspergillus niger bran spores, add 8 times the volume of sterile water, shake and soak for 6 hours, filter with four layers of gauze to obtain a dispersed spore suspension; filter the corn liquefaction solution through a plate and frame to obtain sugar solution Add water to dilute to a total sugar concentration of 10g / L, add starch residue to make the solids concentration reach 50g / L; add 0.08% (v / v) volume Tween 80, adjust the pH to 5.0, and sterilize at 121°C for 15min. Cool to 39°C; insert the spore suspension so that the final concentration of spores is 4.5*10 9 cells / mL, cultivated at a temperature of 38°C and a stirring speed of 50 rpm for 8 hours, then increased the stirring speed to 500 rpm, and stirred for 1 hour to obtain a suspension of germinating spores.

[0043] Seed culture: The seed medium is prepared with corn liquefaction solution and ammonium sulfate, the total sug...

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Abstract

The invention discloses a method for seed culture of Aspergillus niger and preparing citric acid. The method comprises the following steps: (1) Aspergillus niger mouldy bran spores which are cultivated and maturated, in order to obtain dispersed spore suspension; (2) dispersed spore suspension in the step (1) is cultivated in order to obtain germinated spores; (3) germinated spores are dispersed again, the spores are cultivated in a seed tank, in order to obtain matured seed liquid; (5) matured seed liquid is converted to a fermentation tank for cultivation and fermentation, in order to obtaincitric acid fermentation broth. The method solves the aggregation problems in spore germination process, in order to improve conglomeration rate of spores, increase quantity of mycelium pellets, effectively control uniformity of size of mycelium pellets, improve conversion efficiency of thallus, improve conversion rate of fermentation, and shorten fermentation period.

Description

technical field [0001] The invention relates to the technical field of microbial fermentation, in particular to a method for preparing citric acid from specially cultivated Aspergillus niger seed liquid. Background technique [0002] Citric acid is an important organic acid, also known as citric acid. It is colorless, odorless, has a strong sour taste, and is easily soluble in water. It has a wide range of uses in industry, food industry, and cosmetic industry. At present, citric acid is mainly produced by fermentation, using corn and other starchy crops as raw materials. [0003] In traditional citric acid fermentation, the spores of Aspergillus niger are usually expanded to obtain mature spores; then the spores are inserted into the seed medium to obtain mature seed liquid; finally, the seed liquid is transferred to the fermentation medium for fermentation to obtain citric acid fermentation liquid. [0004] Traditional seed cultivation usually takes 24-32 hours, and the c...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12P7/48C12R1/685
CPCC12N1/14C12P7/48
Inventor 石贵阳刘龙陈坚胡志杰蒋建伟蒋小东金赛孙福新李由然周东姣
Owner JIANGSU GUOXIN UNION ENERGY CO LTD
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