Plant cell screening device and a method for screening a synchronized plant cell line

A plant cell line and plant cell technology, applied in the field of cell culture equipment, can solve the problems of slow growth of plant cell culture, long culture period, low planting rate, etc. Effect

Pending Publication Date: 2020-04-28
厦门鹭港兆康生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The object of the present invention is to provide a plant cell filter and a method for screening synchronized plant cell lines to solve the problems of slow plant cell culture growth, long culture cycle, cumbersome operation, low planting rate, and easy pollution

Method used

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  • Plant cell screening device and a method for screening a synchronized plant cell line

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Embodiment 1

[0018] Example 1: See figure 1 , the present embodiment provides a plant cell filter, which is used in conjunction with an aeration device, and includes a tank body 1, a feeding device 7, and a waste liquid collection device 8. The inside of the tank body 1 is provided with a horizontal screen 2, and the screen 2 divides the tank The inner cavity of the body 1 is divided into a connected first cavity and a second cavity, the first cavity is located above the second cavity, and the first cavity is provided with an inoculation port 3, an air outlet 4 and a culture medium interface 5, The second cavity communicates with the feeding device 7 and the waste liquid collecting device 8 , and both the first cavity and the second cavity are provided with air inlets, and the air inlets are connected with the ventilation device 61 .

[0019] The tank body 1 is a tank body made of flexible materials, including but not limited to polyamide, polyethylene, ethylene-vinyl acetate copolymer, et...

Embodiment 2

[0030] Embodiment 2: This embodiment provides a method for screening synchronized plant cell lines to explain the principle of use of the plant cell filter described in Example 1. The method includes the following steps:

[0031] Step 1: Aseptically insert the plant cell seeds into the tank of the plant cell filter that has been fumigated or irradiated beforehand through the inoculation port 3, and at the same time connect the sterilized qualified cell liquid medium through the medium interface 5. Enter the tank body 1, and control the liquid level of the fermented liquid to be higher than the screen cloth 2, open the fourth valve 62 and the first valve 71, the second valve 81 and the third valve 63, the ventilation device will input sterile air into the tank body 1, and simultaneously The sterile air is discharged through the air outlet 4, and placed in a constant temperature room for constant temperature cultivation;

[0032] Step 2: When cultivating until an obvious cell ma...

Embodiment 3

[0037] Example 3: This example provides a method for screening synchronized ginseng cell lines using the plant cell filter disclosed in Example 1, comprising the following steps:

[0038] (1) Aseptically inoculate the ginseng cell callus in a qualified sterilized liquid medium, place it in a 22-25°C, 80-120rpm rotary shaker for 15-25 days, and place the qualified thick porridge-like ginseng The liquid seeds are aseptically inserted into the tank body 1 that has been fumigated or irradiated in advance through the inoculation port 3, and at the same time, the sterilized ginseng cell liquid medium is inserted into the tank body 1 through the medium interface 5 through the peristaltic pump. Note that the liquid level of the fermented liquid is slightly higher than the screen 2, open the fourth valve 62 on the sterile air intake pipeline, close the first valve 71, the second valve 81 and the third valve 63, and the sterile air is input through the air inlet The tank body 1, and the...

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Abstract

The invention discloses a plant cell screening device and a method for screening a synchronized plant cell line, and relates to the field of cell culture equipment. The plant cell screening device isused in cooperation with a ventilation device and comprises a tank body, a material supplementing unit and a waste liquid collecting unit, wherein a horizontal screen is arranged in the tank body anddivides the inner cavity of the tank body into a first cavity and a second cavity which communicate with each other; the first cavity is located above the second cavity; an inoculation opening, an airoutlet and a culture medium connector are arranged on the first cavity; the material supplementing unit and the waste liquid collecting unit communicate with the bottom part of the second cavity; andthe first cavity and the second cavity communicate with the ventilation device. By adoption of the plant cell screening device for screening the synchronized plant cell line, short screening period and high sterility degree are achieved; an obtained cell line has the characteristics of high homogeneity and synchronization degree; the culture period of mass production can be easily shortened; regulation and control of secondary metabolites of mass production can be easily realized; the abundance and stability of metabolites are improved; the fermentation strength is improved; and the production cost is reduced.

Description

technical field [0001] The invention relates to the field of cell culture equipment, in particular to a plant cell filter and a method for screening synchronized plant cell lines. Background technique [0002] Plant-derived natural products are important raw materials in the fields of medicine, food, and chemical industry. The way to obtain natural products from plants is now mainly relying on the extraction of wild plant resources or artificial planting resources. However, most wild plant resources are facing the problem of resource depletion due to over-harvesting. Although artificial planting resources have alleviated the problem of insufficient production of natural products derived from plants to a certain extent, the products from artificial planting often have problems such as unstable content, excessive heavy metals, and excessive pesticide residues. At the same time, artificial planting can easily cause the occupation of forest land and cultivated land , The probl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/00C12M1/00C12N5/04
CPCC12M25/00C12M47/04C12N5/04
Inventor 朱义刚黄涛董伦海
Owner 厦门鹭港兆康生物科技有限公司
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