Method for producing citric acid by using high-strength fermentation technology

A high-strength, citric acid technology, applied in microorganism-based methods, biochemical equipment and methods, fermentation, etc., can solve the problems of low initial total sugar, low conversion rate, long cycle, etc., to promote the growth and production of bacteria. Acid, reduce pollution, increase the effect of fermentation intensity

Active Publication Date: 2013-01-16
RIZHAO JINHE BOYUAN BIOCHEM
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  • Summary
  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In response to the above problems, the present invention proposes a method for producing citric acid by high-intensity fermentation technology, which improves the rate of acid production by fermentation, shortens the fermentation cycle, and can increase acid production, and solves the problem of initial total sugar fermentation in the current citric acid production. low, long cycle, slow acid production, low conversion rate and equipment utilization, etc.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A method for high-intensity fermentation technology to produce citric acid, the steps of which are:

[0027] (1) Grinding corn to prepare corn flour, the 60-mesh transmittance of corn flour after grinding is 50%;

[0028] (2) Mix the corn flour with water, and add high-temperature-resistant α-amylase to it, add 5 enzyme units of high-temperature-resistant α-amylase per gram of corn flour, the concentration of the corn flour after mixing is 22%, and the pH The value is 6.18;

[0029] (3) Transport the adjusted corn flour slurry to a homogenizer for processing, and the particle size of the processed corn flour is 45-50 μm;

[0030] (4) Transfer the treated corn flour slurry to the spray liquefaction device for liquefaction through two continuous spray liquefaction processes. The temperature of the first spray is 95°C, and the temperature of the second spray is 127°C. Maintained for 4 hours, the liquefied DE value is 80%;

[0031] (5) Take the liquefied liquid that acco...

Embodiment 2

[0037] A method for high-intensity fermentation technology to produce citric acid, the steps of which are:

[0038] (1) Grinding corn to prepare corn flour, the 60-mesh transmittance of corn flour after grinding is 60%;

[0039] (2) Mix the corn flour with water, and add high-temperature-resistant α-amylase to it, add 10 enzyme units of high-temperature-resistant α-amylase per gram of corn flour, the concentration of the corn flour after mixing is 10%, and the pH The value is 5.03;

[0040] (3) Transport the adjusted corn flour slurry to a homogenizer for processing, and the particle size of the processed corn flour is 40-42 μm;

[0041] (4) Transfer the treated corn flour slurry to the spray liquefaction device for liquefaction through two continuous spray liquefaction processes. The temperature of the first spray is 97°C, and the temperature of the second spray is 128°C. Maintain for 3 hours, the liquefied DE value is 50%;

[0042](5) Take the liquefied liquid accounting ...

Embodiment 3

[0048] A method for high-intensity fermentation technology to produce citric acid, the steps of which are:

[0049] (1) Grinding corn to prepare corn flour, the 60-mesh transmittance of corn flour after grinding is 70%;

[0050] (2) Mix the corn flour with water, and add high-temperature-resistant α-amylase to it, add 15 enzyme units of high-temperature-resistant α-amylase per gram of corn flour, the concentration of the corn flour after mixing is 35%, and the pH Value is 5.0;

[0051] (3) Transport the adjusted corn flour slurry to a homogenizer for processing, and the particle size of the processed corn flour is 38-43 μm;

[0052] (4) Transfer the treated corn flour slurry to the spray liquefaction device for liquefaction through two continuous spray liquefaction processes. The temperature of the first spray is 96°C, and the temperature of the second spray is 129°C. In the liquefaction laminar flow tank Maintained for 2 hours, the liquefied DE value is 60%;

[0053] (5) T...

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Abstract

The invention relates to a method for producing citric acid by using a high-strength fermentation technology. The method is characterized by comprising the steps as follows: using corns as raw materials; pulverizing and adding water for size mixing; continuously injecting and liquefying twice after preprocessing by a homogenizer or a colloid mill; adding appropriate nitrogen sources and acid proteinase for fermentation at the same time; and adjusting the corresponding production parameters for adaptation. The method not only improves the fermentation acid yield rate, shortens the fermentation period, improves the utilization rate of equipment, improves the economic benefit of enterprises, and promotes the competitiveness of enterprises.

Description

technical field [0001] The invention relates to a method for producing citric acid, in particular to a method for producing citric acid by high-intensity fermentation technology. Background technique [0002] Citric acid, scientific name 2-hydroxypropane-1,2,3-tricarboxylic acid, molecular formula C 6 h 8 o 7 (anhydrous), is an organic acid widely used in food, medicine and chemical industry. With the development of the economy, the demand for citric acid in all walks of life will rise steadily. Of course, the challenges and opportunities faced by various enterprises are also increasing. It is mainly used in food industry, pharmaceutical industry and chemical industry, and it is also widely used in industrial fields such as electronics, textiles, petroleum, leather, construction, photography, plastics, casting and ceramics. [0003] At present, in the production of citric acid, there are shortcomings such as low initial total sugar concentration of fermentation, low aci...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/48C12R1/685
Inventor 寇光智李昌涛蒋水星尹相山周一江梁培森
Owner RIZHAO JINHE BOYUAN BIOCHEM
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