Method for producing L-lactic acid by Bacillus coagulans CGMCC No.2602

A technology of Bacillus coagulans and lactic acid, applied in the field of microorganisms, can solve the problems of nutritional deficiencies and high production costs, and achieve the effects of simple nutritional requirements, reduced fermentation costs, and a wide range of nitrogen sources.

Active Publication Date: 2009-09-30
江苏省苏微微生物研究有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Chinese patent application number: 96121927.0 discloses a method for producing L-lactic acid by Bacillus coagulans, which is characterized by auxotrophy and high production costs

Method used

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  • Method for producing L-lactic acid by Bacillus coagulans CGMCC No.2602
  • Method for producing L-lactic acid by Bacillus coagulans CGMCC No.2602

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1. The process of producing L-lactic acid by semi-continuous batch fermentation

[0024] 1. Cell activation

[0025] Aseptically open the freeze-dried strain of Bacillus coagulans, streak and inoculate on the slant of bran nutrient agar in test tubes, culture at 40-50°C for 18-24 hours, then transfer to the slant of bran nutrient agar eggplant bottle by streaking, 40-60 Cultivate at ℃ for 20-24 hours, check under the microscope, when more than 90% of the bacteria form spores, it is mature and ready for transplantation.

[0026] Slant medium (bran nutrient agar medium): peptone 10g / L, beef extract 3g / L, NaCl 5g / L, bran 10g / L, agar 15-20g / L, distilled water 1L, pH7.0-7.2.

[0027] 2. Seed culture

[0028] 2.1 Incline cultivation

[0029] Use an inoculation loop to dip a small amount of bacteria sludge from the slant of the strain, inoculate it on the slant medium by streaking, culture at 40-60°C for 24-48 hours, and the microscopic examination of the spore r...

Embodiment 2

[0043] Example 2. Production of L-lactic acid by intermediate sugar supplementation fermentation

[0044] 1. Cell activation

[0045] Aseptically open the freeze-dried strain of Bacillus coagulans, streak and inoculate on the slant of bran nutrient agar in test tubes, culture at 40-50°C for 18-24 hours, then transfer to the slant of bran nutrient agar eggplant bottle by streaking, 40-60 Cultivate at ℃ for 20-24 hours, check under the microscope, when more than 90% of the bacteria form spores, it is mature and ready for transplantation.

[0046] Incline medium: peptone 10g / L, beef extract 3g / L, NaCl 5g / L, bran 10g / L, agar 15-20g / L, distilled water 1L, pH7.0-7.2.

[0047] 2. Seed culture

[0048] 2.1 Incline cultivation

[0049] Use an inoculation loop to dip a small amount of bacteria sludge from the slant of the strain, inoculate it on the slant medium by streaking, culture at 40-60°C for 24-48 hours, and microscopically check that the spores > 90% are mature.

[0050] Inc...

Embodiment 3

[0057] Embodiment 3: Corn starch hydrolyzate is the Bacillus coagulans of carbon source and produces L-lactic acid

[0058] Incline medium: peptone 10g / L, beef extract 3g / L, NaCl 5g / L, bran 10g / L, agar 15-20g / L, distilled water 1L, pH7.0-7.2.

[0059] Seed medium: liquefy cornstarch with high-temperature α-amylase and saccharify with glucoamylase to prepare hydrolyzed sugar. The filtrate is used to measure the hydrolyzed sugar by Fehling’s method, with the hydrolyzed sugar at a concentration of 50g / L as the carbon source, peptone 5g / L, and yeast extract 5g / L, (NH 4 ) 2 SO 4 1.0g / L, MnSO 4 ·H 2 O 0.1g / L, calcium carbonate 30g / L, pH5.0-6.0.

[0060] Fermentation medium: liquefy cornstarch with high-temperature α-amylase, saccharify with glucoamylase to prepare hydrolyzed sugar, take the filtrate to measure the hydrolyzed sugar by Fehling’s method, use the hydrolyzed sugar at a concentration of 75-80g / L as the carbon source, and peptone 1.0g / L , barley root 3.0g / L, (NH 4...

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Abstract

A method for producing L-lactic acid by Bacillus coagulans CGMCC No.2602 belongs to the technical field of microorganisms. In the invention, Bacillus coagulans CGMCC No.2602 is adopted, and under the condition of no oxygen supply, starchiness hydrolyzed sugar or dextrose is fermented by a semicontinuous intermittent fermentation way or an inter-sugar-compensating fermentation way to generate L-lactic acid with high optical purity. The invention has the advantages that the gemma property of the Bacillus coagulans is stable, and the L-lactic acid obtained by inoculating and fermenting the starchiness hydrolyzed sugar has high optical purity and rate of conversion of sugar and acid and short fermentation period; in addition, the semicontinuous intermittent fermentation way saves the time for preparing seeds by a continuous reladling and subculturing method, shortens the fermentation period, enhances the fermentation strength and obtains the L-lactic acid with both relatively high rate of conversion of sugar and acid and purity.

Description

technical field [0001] The invention discloses a method for producing L-lactic acid by using Bacillus coagulans CGMCC No.2602, which belongs to the technical field of microorganisms. Background technique [0002] The scientific name of lactic acid is 2-hydroxypropionic acid. It is a chiral molecule with optical activity. The left-handed lactic acid is called L-lactic acid, the right-handed lactic acid is called D-lactic acid, and the racemic lactic acid is called For DL-lactic acid. Since humans and animals only have enzymes to metabolize L-lactic acid, excessive intake of D-lactic acid or DL-lactic acid will lead to rich D-lactic acid in the blood, which will easily cause fatigue, metabolic disorders and even acidosis. L-lactic acid with high optical purity will gradually replace DL-lactic acid in the food and pharmaceutical industries. [0003] L-lactic acid is widely used in food, medicine, agriculture, chemical industry and other fields, covering a large number of prod...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/56C12R1/07
Inventor 匡群孙梅胡凌张维娜施大林陈秋红刘淮
Owner 江苏省苏微微生物研究有限公司
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