Culture method of Saccharomyces diastaticus

A culturing method and saccharification yeast technology, applied in the field of saccharification yeast culture, can solve the problems of high labor intensity and complex process, and achieve the effects of low labor intensity and simple process

A culturing method and saccharification yeast technology, applied in the field of saccharification yeast culture, can solve the problems of high labor intensity and complex process, and achieve the effects of low labor intensity and simple process

CN102477401AInactive Publication Date: 2012-05-30张道玉

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1: Take honey and yeast, add the yeast into the honey, control the ratio of yeast and honey at 1:1—1:10, stir and mix evenly, control the temperature at 14°C—42°C, and assimilate half Hours --- 50 hours.

Embodiment 2

[0019] Example 2: Take sugar and yeast. The sugar includes brown sugar, white sugar, maltose, glucose, and fructose. If it is dry yeast, the sugar can be diluted with water, which is conducive to the assimilation of the yeast. It can also be diluted with honey. The dilution with honey is more than the dilution with water. Even better, if it is wet yeast, the sugar can be diluted without adding water or honey, and the sugar concentration is controlled at 60%-90%; after adding the yeast to the sugar and mixing it evenly, the temperature is controlled at 14°C-42°C, and it is still assimilated Half an hour---50 hours.

[0020] The said stillness is exactly to stop stirring in the meantime.

[0021] The assimilation is to soak the yeast in honey liquid or sugar liquid, and the honey liquid or sugar liquid penetrates into the cells of the yeast to change the properties of the yeast, improve the fermentation power of the yeast, saccharify the starch, and make the wine astringent The...

Embodiment 3

[0022] Example 3: Take honey and water, the ratio of honey and water is controlled at 1:2-1:30, stir and mix evenly, add assimilative yeast as culture medium, and control the ratio of assimilated yeast to culture medium at 1:10-1 : 50, the fermentation temperature is controlled at 14°C - 42°C, the fermentation time is controlled at 30 hours - 500 hours, static fermentation culture.

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PUM

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Abstract

The invention relates to a culture method of Saccharomyces diastaticus and in particular relates to a culture method of yeast. The method comprises the following steps of: assimilating yeast with saccharides, and inoculating the assimilated yeast to a culture fluid for fermentation culture. After being assimilated with honey or sugar, the original properties of yeast are changed, so that yeast which can not directly act on starch can directly act on starch. By fermentation culture of yeast in the culture fluid supplemented with honey, water or a mixture of sugar and water, the fermentation strength of yeast is improved and the continuous assimilation effect is achieved. Owing to assimilation fermentation culture of yeast in the culture fluid supplemented with honey and sugar, the brewed wine tastes less astringent and bitter and has better palatability. The yeast cultured by the method can directly act on starch, achieves synchronization of saccharification and fermentation and integrates the functions of saccharification and fermentation, so as to omit the use of Daqu starter, Xiaoqu starter, bran koji, saccharifying enzymes or malt and reduce labor intensity. The invention has the advantage that the process of culturing Saccharomyces diastaticus is simple and has low labor intensity.

Description

technical field [0001] The invention relates to a method for cultivating yeast, in particular to a method for cultivating saccharification yeast. Background technique [0002] At present, Daqu, Xiaoqu, bran koji, and glucoamylase are mostly used in winemaking, and maltosaccharified starch is also used for winemaking. [0003] The Daqu technology used in brewing now, taking Daqu as an example: [0004] 1. Choose raw materials that are free from mildew, sprouting, and clean. Generally, wheat, barley, peas, etc. are used, and then the raw materials are crushed. Fast volatilization of the cross-section produces heart, the microbial growth of the koji is not good, the crushing is too fine, the pressed koji is highly viscous, the voids in the koji are small, and the water heat is not easy to lose, which will cause the phenomenon of retting, bulging, and old circles, so the thickness of the crushing must be certain. It should be moderate. Some units of wheat, barley and peas pass...

Claims

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Application Information

Patent Timeline
30 May 2012
Publication
CN102477401A
IPC
C12N1/16; C12R1/645
Inventors
张道玉