Potato isolated culture one-step seedling culture medium and optimization method and seedling method thereof

A technology of in vitro culture and culture medium, applied in the biological field, can solve the problems of short regeneration cycle and achieve the effect of short regeneration cycle, simple culture procedure and easy operation
CN101790935AInactive Publication Date: 2010-08-04SICHUAN AGRI UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SICHUAN AGRI UNIV
Publication Date
2010-08-04
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a method for optimizing a potato isolated culture one-step seedling culture medium. The culture medium makes leaves of a potato test tube plantlet undergo isolated culture to form a seedling in one step. The one-step seedling culture medium takes an MS culture medium as a basal medium, and 6-benzyladenine, naphthyl acetic acid and 2,4-dichlorphenoxyacetic acid with different concentrations and combinations are supplied in 1L of the MS culture medium; adventitious buds and adventitious roots are directly differentiated through callus induction; and the potato regenerated seedling is obtained in one step. The callus obtained by induction in a primary culture medium is unnecessarily inoculated to a differential medium for regenerating the seedling. Compared with an isolated culture multi-step regenerated seedling method, the method of the invention has the advantages of simplified steps, short culture period, high repeatability, and high seedling survival rate.
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Description

technical field

[0001] The invention belongs to the field of biotechnology, and in particular relates to a medium for one-step seedling growth in vitro culture of potatoes, and a method for making seedlings of potato test-tube seedlings in one step by using the medium. Correspondingly, the present invention also provides a method for optimizing the Medium method. Background technique

[0002] Potato (Solanun tuberosum L.) is an important grain, vegetable, feed and industrial raw material and crop, and occupies a very important position in my country's agricultural production. In order to innovate germplasm resources, tissue culture technology has been widely used in potato breeding research, and has achieved remarkable results.

[0003] Plant tissue culture generally adopts several cultivation procedures such as dedifferentiation, redifferentiation buds and rooting strong seedlings, which are called multi-step seedling formation. In order to simplify the cultivation proced...

Claims

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