Rapid propagation method of Longya lilies

A dragon tooth lily, fast technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve problems such as increased workload, repeated pollution, and bacterial infection of bulbs, and achieve shortened regeneration cycle, increased regeneration efficiency, and improved The effect of induction rate

Inactive Publication Date: 2016-01-06
韦丽
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is relatively convenient to use bulbs as explants for regeneration, but due to the serious bacterial contamination of bulbs, it often leads to incomplete disinfection, repeated pollution during the cultivation process is very serious, increasing the workload, and the regeneration efficiency of scales as explants is low. Not very ideal

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] A kind of rapid propagation method of lily of dragon tooth, comprises the steps:

[0022] 1) Take the bulbs of Lilium japonica planted in the open field for 2 months, select healthy and intact bulbs, and peel off the outer scales;

[0023] 2) After soaking the bulb scale concentration obtained in step 1) in a potassium permanganate solution of 5% for 15 minutes, wash it with clear water;

[0024] 3) peeling off the seed buds from the bulb scales obtained in step 2), laying flat on MS solid medium to induce growth of adventitious buds, and obtaining tissue blocks with adventitious bud clusters;

[0025] 4) making the tissue block obtained in step 3) into a longitudinally divided tissue block, and carrying out bud elongation culture on the bud elongation medium;

[0026] 5) When the adventitious buds obtained in step 4) elongate and form seed bulbs, they are cut and put into rooting medium for rooting induction culture to obtain Lilium japonicus bulbs.

[0027] The MS s...

Embodiment 2

[0034] A kind of rapid propagation method of lily of dragon tooth, comprises the steps:

[0035] 1) Take the bulbs of lily lily planted in the open field for 2.5 months, select healthy and intact bulbs, and peel off the outer scales;

[0036] 2) Soak the bulb scales obtained in step 1) in a potassium permanganate solution with a concentration of 6% for 18 minutes, and then wash them off with water;

[0037] 3) peeling off the seed buds from the bulb scales obtained in step 2), laying flat on MS solid medium to induce growth of adventitious buds, and obtaining tissue blocks with adventitious bud clusters;

[0038] 4) making the tissue block obtained in step 3) into a longitudinally divided tissue block, and carrying out bud elongation culture on the bud elongation medium;

[0039] 5) When the adventitious buds obtained in step 4) elongate and form seed bulbs, they are cut and put into rooting medium for rooting induction culture to obtain Lilium japonicus bulbs.

[0040] The ...

Embodiment 3

[0047] A kind of rapid propagation method of lily of dragon tooth, comprises the steps:

[0048] 1) Take the bulbs of lily lily planted in the open field for 3 months, select healthy and intact bulbs, and peel off the outer scales;

[0049] 2) Soak the bulb scales obtained in step 1) in a potassium permanganate solution with a concentration of 7% for 20 minutes, and then wash them off with water;

[0050] 3) peeling off the seed buds from the bulb scales obtained in step 2), laying flat on MS solid medium to induce growth of adventitious buds, and obtaining tissue blocks with adventitious bud clusters;

[0051] 4) making the tissue block obtained in step 3) into a longitudinally divided tissue block, and carrying out bud elongation culture on the bud elongation medium;

[0052] 5) When the adventitious buds obtained in step 4) elongate and form seed bulbs, they are cut and put into rooting medium for rooting induction culture to obtain Lilium japonicus bulbs.

[0053] The MS...

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PUM

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Abstract

The invention relates to a rapid propagation method of Longya lilies. The method comprises steps as follows: a seed ball of the Longya lilies is taken, a tissue block with an adventitious bud cluster is obtained after inducted growth culture of adventitious buds, and Longya lily seedlings are obtained after induced rooting culture. The rapid propagation method has the benefits as follows: the regeneration efficiency of the method is increased, the bud ratio reaches about 100%, the pollution rate is reduced, the repeated pollution situation cannot occur in the culture process, the induction rate can be increased and the regeneration cycle can be shortened by adjusting culture medium conditions, and the quality and the yield of the Longya lilies are effectively improved.

Description

technical field [0001] The invention relates to a method for breeding and expanding the seeds of the biological medicinal material Lilium japonicus. The technology is directly applied to the breeding and expansion of the biological medicinal material, and in particular relates to a rapid propagation method for the lily of the longya. Background technique [0002] Longya lily is planted in Jiangxi (Wanzai, Yongfeng Zhongxishan), Hunan (Longhui, Anhua) and other places. Among them, Jiangxi Wanzai Baishui Township is particularly well-known. Longya lily is a well-known product in Wanzai. The planting history According to county chronicles, it can be traced back to the Song Dynasty, and its Longya lily products were tributes of all dynasties; Longya lily is an excellent variety among white lilies, and it is also the variety with the best nutrition. Lily (Lily albiflora) bulbs are spherical, 2-2.4 cm in transverse diameter, scales are lanceolate, white, yellowish white or slightl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 韦丽
Owner 韦丽
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