One-step seedling emergence and quick propagation method for tissue culture of sedum aizoon

A tissue culture and cabbage raising technology, applied in the field of plant tissue culture, can solve the problems of easy mutation of germplasm, cumbersome pathways, low regeneration frequency, etc., and achieve the effects of short regeneration cycle, maintaining original species characteristics, and easy operation.

Active Publication Date: 2015-01-07
RIZHAO POLYTECHNIC
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Problems solved by technology

However, this approach is cumbersome, the germplasm is pro

Method used

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  • One-step seedling emergence and quick propagation method for tissue culture of sedum aizoon
  • One-step seedling emergence and quick propagation method for tissue culture of sedum aizoon
  • One-step seedling emergence and quick propagation method for tissue culture of sedum aizoon

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specific Embodiment approach 1

[0017] Specific implementation mode 1: This implementation mode will further describe and illustrate the present invention in conjunction with specific examples below. Described method is carried out according to the following steps:

[0018] Step 1, take the leaves of Chinese cabbage that are free from diseases and insect pests and grow robustly as explants, and then sterilized;

[0019] Step 2. Induction of leaf callus: Cut the sterilized leaves into 0.4-0.6cm2 blocks and inoculate them on the culture medium. After the inoculation, the culture medium is placed at a temperature of 25±2°C. Dark culture, after 7 to 10 days, carry out light culture until rooting;

[0020] Step 3. Domestication and transplanting of test-tube seedlings: Take out the rooted seedlings and put them in a hardening room with sufficient light. After 3 to 5 days, open the bottle and continue to harden the seedlings for 2 to 3 days; then take out the test-tube seedlings and wash the roots for cultivation...

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Abstract

The invention relates to a one-step seedling emergence and quick propagation method for the tissue culture of sedum aizoon, and belongs to the technical field of plant tissue culture. Through the adoption of the method disclosed by the invention, the problems that the coefficient of a conventional propagation method for the sedum aizoon is low, the growth is slow, and the large-scale production is difficult to form are solved. The method comprises the following steps: (1) performing sterilization and disinfection; (2) performing one-step seedling emergence culture; (3) domesticating and transplanting test-tube plantlets, wherein rooting seedlings are taken out and are put in a seedling exercising room with enough illumination, after 3-5 days, bottle mouths are opened, seedlings are continuously exercised for 2-3 days, then the test-tube plantlets are taken out, root culture mediums are cleaned and are transplanted in substrates consisting of peat soil and vermiculite, the ratio of the weight parts of the peat soil to the weight parts of the vermiculite is 1: 1, and the condition of spraying small seedlings 2-3 times a day for first 7 days is ensured. The method disclosed by the invention is used for the tissue culture of the sedum aizoon.

Description

technical field [0001] The invention relates to a one-step seedling rapid propagation method for tissue culture of Chinese cabbage, belonging to the technical field of plant tissue culture. Background technique [0002] At present, the production and propagation of Chinese cabbage is mainly based on division propagation and cutting propagation. The details are as follows: 1. Cuttage propagation selects strong branches that grow in the same year, cuts into 10-15cm long stem segments, removes the base leaves, leaves 3.4 leaves in each stem segment, tops, inserts in the furrow, fills the soil with 3-5cm, and pours it once Water permeable, generally takes 7-10 days to take root and survive, the branches are best cut and planted, and can be transplanted and planted after 20 days. It can also be cut directly in the field, the planting density is 25cm row spacing, 15cm hole spacing, 2-3 plants per hole. 2. Propagation by ramets Dig up the roots of the Chinese cabbage to be ramete...

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Application Information

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IPC IPC(8): A01H4/00
Inventor 李媛侯可雷
Owner RIZHAO POLYTECHNIC
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