6 results about "Rubus coreanus" patented technology

The invention discloses a rubus cochinchinensis efficient genetic transformation method based on RUBY reporter gene and agrobacterium rhizogenes mediation, which realizes that the transformation period is shortened to be within 15 days and the transformation efficiency is improved to be more than 60% through direct explant treatment under a non-tissue culture condition. The method comprises the following specific steps: 1) preparing an explant material; (2) constructing an RUBY carrier and transforming agrobacterium rhizogenes; (3) infecting the explant by utilizing agrobacterium rhizogenes carrying a target gene; (4) co-culturing after infection and carrying out RUBY visual screening; 5) positive root molecular biology verification; (6) inducing positive roots into buds; according to the method, induction of the positive callus, the positive root and the positive bud of the rubus coreanus stem segment under the non-tissue culture condition is successfully achieved, a stable and efficient genetic transformation system is constructed, the method has the remarkable advantages of being easy and convenient to operate, low in cost, wide in applicability, high in reproducibility and the like, and a key technical support is provided for molecular breeding of the rubus coreanus.

1. Name of the product in this design: Label (Prickly Pear and Passion Fruit Compound Flavor Beverage). 2. Application of this design: This design is intended for use as a label on any one or more types of packaging bottles, bags, boxes, and cartons. 3. The key design feature of this product is its pattern. 4. The image or photograph that best illustrates the design's key features: the front view. 5. Other matters requiring explanation: This design is a two-dimensional product, so the left view, right view, top view, and bottom view are omitted; the back of this design is a part that is not easily seen or cannot be seen during use, so the rear view is omitted.

A fermented natural substance having nitric oxide metabolites fixated and stabilized therein is produced by a process including adding a fermentative strain to nitrogen-containing natural substance and carrying out fermentation by a batch fermentation process. The nitrogen-containing natural substance is at least one selected from the group consisting of lettuce, Sedum sarmentosum, spinach, blueberry, dandelion, pomegranate, cabbage, garlic, Morinda citrifolia, onion, soybean, bean sprout, mulberry leaf, Momordica charantia, Rubus coreanus, Houttuynia cortada, aronia, Humulus japonicus, Coptis chinensis, hijiki, hooker chive, mushrooms, calamus, wild spinach (Seomcho), Chinese cabbage, kelp, apple, mugwort, orange, salmon testis, abalone shell, shellfish shell, cricket, silkworm, Rehmannia glutinosa, and Shipjeondaebo-tang. to Bacillus sp., Bifidobacterium sp., Enterococcus sp., or Lactobacillus sp.

The invention discloses a tissue culture high-quality seedling propagation method for rosaceae rubus L plant rosaceae rubus L, which is suitable for tissue culture and rapid propagation of rosaceae rubus L plant rosaceae rubus L, and belongs to the technical field of plant biology. The teasel-free rubus coreanus is a medicinal and edible small berry which is widely planted in China at present, the teasel-free rubus coreanus is obtained through the steps of callus induction and differentiation, adventitious bud rooting and seedling strengthening, test-tube plantlet transplanting and the like by utilizing an optimized culture medium formula and an aseptic seedling propagation method by taking a sterilized teasel-free rubus coreanus branch stem section as an explant, and the teasel-free rubus coreanus is obtained through the steps of rooting and seedling strengthening of adventitious buds, test-tube plantlet transplanting and the like. And a tissue culture and rapid propagation system of the high-quality seedlings of the rubus coreanus is established for the first time. The method has the characteristics of simple plant tissue culture equipment, high success rate, high propagation efficiency, good seedling quality and the like. The method provides technical support for large-scale production of the rubus coreanus, and has a good market prospect.

The invention discloses an application of dendrobium huoshanense protocorm in resisting fatigue, invigorating kidney and strengthening yang. The dendrobium huoshanense protocorm is combined with the herba cistanche, the American ginseng, the raspberry, the dogwood and the fructus lycii, so that the anti-fatigue, kidney-tonifying and yang-strengthening effects of the formula can be remarkably improved. Compared with the single use of cistanche, after the addition of the dendrobium huoshanense protocorm, the exhaustive swimming time of a mouse can be obviously prolonged, the content of urea nitrogen and lactic acid in serum of the mouse is reduced, the content of hepatic glycogen in hepatic tissue is improved, and the content of muscle glycogen in muscular tissue is improved. Compared with a single use of cistanche, the dendrobium huoshanense protocorm can significantly improve the activity of superoxide dismutase in serum of rats with kidney-yang deficiency, reduce the content of malondialdehyde in the serum, improve the content of testosterone in the serum, improve the activity of nitric oxide synthase in penis tissues, and improve the content of nitric oxide and cyclic guanosine monophosphate in the penis tissues after the addition of the dendrobium huoshanense protocorm.

The invention discloses rhodobryum roseum polysaccharide as well as a preparation method and application thereof. The molar content of glucose in monosaccharide composition of the rhodobryum roseum polysaccharide is not lower than 97%, and the rhodobryum roseum polysaccharide contains terminal and 1-6, 1-4, 1-4, 6, 1-3 and 6 connected glucose residues. The preparation method comprises the following steps: degreasing a rhodobryum roseum medicinal material with acetone, adding hot water, carrying out ethanol gradient precipitation, Sevag-method deproteinization and dialysis impurity removal on an extracting solution, and purifying through a DEAE-52 cellulose column and a Sephadex G-75 gel column in sequence to obtain the rhodobryum roseum extract. Experiments prove that the polysaccharide can remarkably improve spleen and thymus indexes and serum IgA, IgG and IgM levels of immunosuppressed mice and shows excellent humoral immune enhancement activity, and the action mechanism of the polysaccharide is related to regulation and control of signal channels such as PI3K / Akt. The invention also provides application of the polysaccharide in preparation of an immunomodulator and a pharmaceutical composition.