9 results about "Rubus coreanus" patented technology

The invention discloses a rubus cochinchinensis efficient genetic transformation method based on RUBY reporter gene and agrobacterium rhizogenes mediation, which realizes that the transformation period is shortened to be within 15 days and the transformation efficiency is improved to be more than 60% through direct explant treatment under a non-tissue culture condition. The method comprises the following specific steps: 1) preparing an explant material; (2) constructing an RUBY carrier and transforming agrobacterium rhizogenes; (3) infecting the explant by utilizing agrobacterium rhizogenes carrying a target gene; (4) co-culturing after infection and carrying out RUBY visual screening; 5) positive root molecular biology verification; (6) inducing positive roots into buds; according to the method, induction of the positive callus, the positive root and the positive bud of the rubus coreanus stem segment under the non-tissue culture condition is successfully achieved, a stable and efficient genetic transformation system is constructed, the method has the remarkable advantages of being easy and convenient to operate, low in cost, wide in applicability, high in reproducibility and the like, and a key technical support is provided for molecular breeding of the rubus coreanus.

1. Name of the product in this design: Label (Prickly Pear and Passion Fruit Compound Flavor Beverage). 2. Application of this design: This design is intended for use as a label on any one or more types of packaging bottles, bags, boxes, and cartons. 3. The key design feature of this product is its pattern. 4. The image or photograph that best illustrates the design's key features: the front view. 5. Other matters requiring explanation: This design is a two-dimensional product, so the left view, right view, top view, and bottom view are omitted; the back of this design is a part that is not easily seen or cannot be seen during use, so the rear view is omitted.

A fermented natural substance having nitric oxide metabolites fixated and stabilized therein is produced by a process including adding a fermentative strain to nitrogen-containing natural substance and carrying out fermentation by a batch fermentation process. The nitrogen-containing natural substance is at least one selected from the group consisting of lettuce, Sedum sarmentosum, spinach, blueberry, dandelion, pomegranate, cabbage, garlic, Morinda citrifolia, onion, soybean, bean sprout, mulberry leaf, Momordica charantia, Rubus coreanus, Houttuynia cortada, aronia, Humulus japonicus, Coptis chinensis, hijiki, hooker chive, mushrooms, calamus, wild spinach (Seomcho), Chinese cabbage, kelp, apple, mugwort, orange, salmon testis, abalone shell, shellfish shell, cricket, silkworm, Rehmannia glutinosa, and Shipjeondaebo-tang. to Bacillus sp., Bifidobacterium sp., Enterococcus sp., or Lactobacillus sp.

The invention discloses a tissue culture high-quality seedling propagation method for rosaceae rubus L plant rosaceae rubus L, which is suitable for tissue culture and rapid propagation of rosaceae rubus L plant rosaceae rubus L, and belongs to the technical field of plant biology. The teasel-free rubus coreanus is a medicinal and edible small berry which is widely planted in China at present, the teasel-free rubus coreanus is obtained through the steps of callus induction and differentiation, adventitious bud rooting and seedling strengthening, test-tube plantlet transplanting and the like by utilizing an optimized culture medium formula and an aseptic seedling propagation method by taking a sterilized teasel-free rubus coreanus branch stem section as an explant, and the teasel-free rubus coreanus is obtained through the steps of rooting and seedling strengthening of adventitious buds, test-tube plantlet transplanting and the like. And a tissue culture and rapid propagation system of the high-quality seedlings of the rubus coreanus is established for the first time. The method has the characteristics of simple plant tissue culture equipment, high success rate, high propagation efficiency, good seedling quality and the like. The method provides technical support for large-scale production of the rubus coreanus, and has a good market prospect.

Provided is a method of manufacturing a health functional food, which includes step (S1) of preparing a red ginseng fermentation powder mixed with red ginseng, black ginseng, ginger, bellflower, and jujube, step (S2) of preparing a medicinal powder mixed with Bikal skullcap (Scutellaria baicalensis), Amur Corktree (Phellodendron amurense), gardenia (Gardenia jasminoides), Purslane (Portulaca oleracea), Chameleon plant (Houttuynia cordata), tea plant (Camellia sinensis), Beefsteak plant (Perilla frutescens var. crispa), Cuscuta chinensis Lam, Cistanche Deserticola, Asparagus cochinchinensis, rehmania root (Rehmannia glutinosa), steamed rehmania root, Thuja Orientalis Seed (Seed of Platycladus orientalis fruit), Rubus coreanus, Reishi mushroom (Ganoderma lingzhi), and Acorus gramineu, step (S3) of preparing a grain mixture mixed with rice, white rice barley (Hordeum distichon), glutinous rice, corn, Foxtail millet (Setaria italica), black sesame, perilla, Coix lacryma-jobi, brown rice, sorghum, fermented soybean, Canavalia ensiformis, chickpea (Cicer arietinum), buckwheat, green gram (Vigna radiata), and oat, step (S4) of preparing a vegetable mixture mixed with sea bamboo shoot, carrot, burdock root, lotus root, cinnamon, water parsley (Oenanthe), broccoli, kelp, and cabbage.

The invention discloses an application of dendrobium huoshanense protocorm in resisting fatigue, invigorating kidney and strengthening yang. The dendrobium huoshanense protocorm is combined with the herba cistanche, the American ginseng, the raspberry, the dogwood and the fructus lycii, so that the anti-fatigue, kidney-tonifying and yang-strengthening effects of the formula can be remarkably improved. Compared with the single use of cistanche, after the addition of the dendrobium huoshanense protocorm, the exhaustive swimming time of a mouse can be obviously prolonged, the content of urea nitrogen and lactic acid in serum of the mouse is reduced, the content of hepatic glycogen in hepatic tissue is improved, and the content of muscle glycogen in muscular tissue is improved. Compared with a single use of cistanche, the dendrobium huoshanense protocorm can significantly improve the activity of superoxide dismutase in serum of rats with kidney-yang deficiency, reduce the content of malondialdehyde in the serum, improve the content of testosterone in the serum, improve the activity of nitric oxide synthase in penis tissues, and improve the content of nitric oxide and cyclic guanosine monophosphate in the penis tissues after the addition of the dendrobium huoshanense protocorm.

The invention discloses rhodobryum roseum polysaccharide as well as a preparation method and application thereof. The molar content of glucose in monosaccharide composition of the rhodobryum roseum polysaccharide is not lower than 97%, and the rhodobryum roseum polysaccharide contains terminal and 1-6, 1-4, 1-4, 6, 1-3 and 6 connected glucose residues. The preparation method comprises the following steps: degreasing a rhodobryum roseum medicinal material with acetone, adding hot water, carrying out ethanol gradient precipitation, Sevag-method deproteinization and dialysis impurity removal on an extracting solution, and purifying through a DEAE-52 cellulose column and a Sephadex G-75 gel column in sequence to obtain the rhodobryum roseum extract. Experiments prove that the polysaccharide can remarkably improve spleen and thymus indexes and serum IgA, IgG and IgM levels of immunosuppressed mice and shows excellent humoral immune enhancement activity, and the action mechanism of the polysaccharide is related to regulation and control of signal channels such as PI3K / Akt. The invention also provides application of the polysaccharide in preparation of an immunomodulator and a pharmaceutical composition.

According to the present invention, a method for preparing a health functional food having the excellent effects of preventing hair loss, promoting hair growth, strengthening hair roots, darkening hair, and improving scalp comprises the steps of: (S1) preparing a red ginseng fermented powder in which red ginseng, black ginseng, ginger, Platycodon grandiflorum, and jujube are mixed; (S2) preparing a medicinal powder in which Scutellaria baicalensis, Phellodendron amurense, Gardenia jasminoides, Portulaca oleracea, Houttuynia cordata, Camellia sinensis, Perilla frutescens, Cuscuta chinensis, Cistanche deserticola, Asparagus cochinchinensis, Rehmanniae radix crudus, Rehmanniae radix preparata, Thuja orientalis, Rubus coreanus, Ganoderma licidum and Acorus gramineus are mixed; (S3) preparing a grain mixture in which rice, white glutinous barley, glutinous rice, corn, foxtail millet, black sesame, perilla seeds, adlay millet, brown rice, great millet, soybeans, sword beans, chickpeas, buckwheat, mung beans and oats are mixed; and (S4) preparing a vegetable mixture in which bamboo shoots, carrots, burdock root, lotus root, cinnamon, dropwort, broccoli, kelp and cabbage are mixed.

The present invention relates to a method for producing a fermented Rubus coreanus concentrate. More specifically, the present invention relates to a method for producing a fermented Rubus coreanus concentrate, which enables reproduction of a traditional method using decoction in a cast-iron cauldron and, at the same time, can provide a product having a unique flavor and taste of Rubus coreanus. To this end, the present invention provides a method for producing a fermented Rubus coreanus concentrate, the method comprising: (a) a fresh Rubus coreanus fruit selection and primary processing step of selecting non-frozen native Rubus coreanus and removing leaves and thorns to obtain only fresh fruits; (b) a grinding step of grinding the fresh Rubus coreanus fruits with seeds by using a grinder to a predetermined particle size; (c) a decoction step of preparing a cast-iron cauldron decoction device, adding the ground fresh Rubus coreanus fruits to the cast-iron cauldron decoction device, and decocting by heating; and (d) a fermentation and aging step of placing the Rubus coreanus decoction solution obtained in the decoction step (c) in an unglazed jar and fermenting and aging same at a temperature of 15-18 °C for 3 months so that the unique ingredients of Rubus coreanus can be adhered and stabilized.