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Efficient euonymus fortunei regeneration culture medium group

A regeneration medium, the technology of Fufangteng, which is applied in the field of plant hormones, can solve the problems of no regeneration medium group of Fufangteng, and achieve good rooting effect and growth promotion effect

Inactive Publication Date: 2017-11-03
FOSHAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The regeneration process of Fufang vine includes subculture of callus, induced growth of adventitious buds, subculture of regenerated seedlings and rooting of regenerated seedlings. However, there is still no ideal and efficient regeneration medium group suitable for Fufang vine in the prior art.

Method used

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  • Efficient euonymus fortunei regeneration culture medium group
  • Efficient euonymus fortunei regeneration culture medium group
  • Efficient euonymus fortunei regeneration culture medium group

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1: callus subculture medium

[0020] Using MS as the basic medium, adding different concentrations of NAA and BA ratio growth regulators, adjusting the pH of the medium to 5.8-7.0, 3% sucrose, 0.8% agar, BA concentration gradient of 1.0-5.0mg / L and NAA Concentration gradient of 0.05 ~ 0.5mg / L was prepared to establish 16 kinds of callus subculture medium, the number and formula of which are as follows:

[0021] N11 medium MS+NAA0.05mg / L+BA1.0mg / L

[0022] N12 medium MS+NAA0.05mg / L+BA1.5mg / L

[0023] N13 medium MS+NAA0.05mg / L+BA2.0mg / L

[0024] N14 medium MS+NAA0.05mg / L+BA5.0mg / L

[0025] N21 medium MS+NAA0.1mg / L+BA1.0mg / L

[0026] N22 medium MS+NAA0.1mg / L+BA1.5mg / L

[0027] N23 medium MS+NAA0.1mg / L+BA2.0mg / L

[0028] N24 medium MS+NAA0.1mg / L+BA5.0mg / L

[0029] N31 medium MS+NAA0.2mg / L+BA1.0mg / L

[0030] N32 medium MS+NAA0.2mg / L+BA1.5mg / L

[0031] N33 medium MS+NAA0.2mg / L+BA2.0mg / L

[0032] N34 medium MS+NAA0.2mg / L+BA5.0mg / L

[0033] N41 medium MS+N...

Embodiment 2

[0042] Embodiment 2: Induce bud growth medium

[0043]Use MS as the basic medium, add different concentrations of NAA and BA growth regulators, adjust the pH of the medium to 5.8-7.0, 3% sucrose, 0.8% agar, BA0.1-1.0mg / L and NAA0-1.0 mg / L was formulated to establish 25 kinds of inducing bud growth media, and their serial numbers and formulas are as follows:

[0044] B01 Medium MS

[0045] B02 medium MS+BA0.1mg / L

[0046] B03 medium MS++BA0.2mg / L

[0047] B04 Medium MS+BA0.5mg / L

[0048] B05 medium MS+BA1mg / L

[0049] B11 Medium MS+NAA0.1mg / L

[0050] B12 medium MS+NAA0.1mg / L+BA0.1mg / L

[0051] B13 medium MS+NAA0.1mg / L+BA0.2mg / L

[0052] B14 medium MS+NAA0.1mg / L+BA0.5mg / L

[0053] B15 medium MS+NAA0.1mg / L+BA1mg / L

[0054] B21 Medium MS+NAA0.2mg / L+BA0mg / L

[0055] B22 medium MS+NAA0.2mg / L+BA0.1mg / L

[0056] B23 medium MS+NAA0.2mg / L+BA0.2mg / L

[0057] B24 medium MS+NAA0.2mg / L+BA0.5mg / L

[0058] B25 medium MS+NAA0.2mg / L+BA1mg / L

[0059] B31 medium MS+NAA0.5mg / L+BA0mg...

Embodiment 3

[0076] Embodiment 3: subculture medium of regenerated shoots

[0077] Using MS as the basic medium, adding different concentrations of NAA and BA ratio growth regulators, adjusting the pH of the medium to 5.8-7.0, 3% sucrose, 0.8% agar, BA (0-2.0 mg / L) and NAA ( 0~1.0mg / L) to prepare 8 kinds of subculture media for regenerated seedlings, the numbers and formulas of which are as follows:

[0078] MS as blank control

[0079] D1 medium MS+BA0.5mg / L

[0080] D2 medium MS+BA1.0mg / L

[0081] D3 Medium MS+BA1.5mg / L

[0082] D4 medium MS+BA2.0mg / L

[0083] D5 medium MS+BA0.1mg / L+NAA0.01mg / L

[0084] D6 medium MS+BA0.1mg / L+NAA0.05mg / L

[0085] D7 medium MS+BA0.1mg / L+NAA0.1mg / L

[0086] On the ultra-clean workbench, inoculate the subcultures with adventitious buds on the above-mentioned 8 kinds of subculture media for regenerated seedlings. Take 8 bottles of each kind, and each bottle has 3 to 4 buds. The cultivation conditions were the same as above. And after 20 days, obser...

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Abstract

The invention discloses an efficient euonymus fortunei regeneration culture medium group. The efficient euonymus fortunei regeneration culture medium group is composed of a callus subculture medium, an inducing bud growth culture medium, a regenerated seedling subculture medium and a regenerated seedling rooting culture medium, wherein the callus subculture medium contains 0.5mg / L of NAA (Naphthylacetic acid) and 1mg / L to 1.5mg / L of BA (Butyl Acrylate); the inducing bud growth culture medium contains 0.1mg / L of the NAA and 0.5mg / L of the BA; the regenerated seedling subculture medium contains 0.05mg / L of the NAA and 1mg / L of the BA; the regenerated seedling rooting culture medium contains 0.6mg / L of the NAA. By adopting the callus subculture medium in the culture medium group disclosed by the invention, euonymus fortunei calluses can grow relatively rapid and the calluses with good differentiation and inducing capabilities are cultured; the inducing rate of the inducing bud growth culture medium on euonymus fortunei adventitious buds reaches 16.7 percent; the regenerated seedling subculture medium has a remarkable promotion effect on the growth of euonymus fortunei regenerated seedlings and the regenerated seedling rooting culture medium has a good rooting effect on euonymus fortunei.

Description

technical field [0001] The invention relates to the field of plant hormones, in particular to a growth regulator of Fufangteng and a preparation method thereof. Background technique [0002] Phytohormones are regulatory substances produced by plants themselves. Such substances generally regulate plant growth at low concentrations, and usually move from the production site to the action site in the plant. Currently recognized plant hormones have five categories, they are: auxins, cytokinins, gibberellins, abscisic acid and ethylene. The mechanism of action of phytohormones is as follows: After the hormones in plants bind to certain proteins called hormone receptors in cells, they can regulate metabolism. Hormone receptors have strong specificity and affinity with hormones. Some receptors exist on the plasma membrane, and after binding with auxin, they change the activity of the proton pump on the plasma membrane and affect membrane permeability. Some receptors exist in the...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001
Inventor 文锦欢萧洪东王惠珍邓日烈
Owner FOSHAN UNIVERSITY
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