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A method for inducing adventitious buds and plant regeneration of Rhododendron xing'an

A bud induction and plant technology, applied in the field of plant tissue culture, can solve the problems of difficulty in culturing organs, long time, etc., and achieve the effects of broad application prospects, consistency, and simple operation steps.

Active Publication Date: 2021-06-18
黑龙江岭山农业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The present invention aims to solve the problem of difficult and long-time-consuming tissue culture organs of the existing Rhododendron xing'an, and provides a method for inducing adventitious buds and plant regeneration of Rhododendron xing'an

Method used

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  • A method for inducing adventitious buds and plant regeneration of Rhododendron xing'an
  • A method for inducing adventitious buds and plant regeneration of Rhododendron xing'an
  • A method for inducing adventitious buds and plant regeneration of Rhododendron xing'an

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specific Embodiment approach 1

[0033] Specific embodiment one: the method for induction of adventitious buds and plant regeneration of rhododendron xing'an in this embodiment comprises the following steps:

[0034] 1. Material collection and processing:

[0035] Select wild Xing’an Rhododendron plants that are robust and free from diseases and insect pests, cut off 40-50cm long branches, and perform hydroponics treatment. Cut off when the tender stems grow to 3-5cm, rinse with tap water for 1-2 hours, and place them on a sterile operating table. Disinfect inside;

[0036] 2. Adventitious bud differentiation induction and proliferation:

[0037] Inoculate the sterilized materials in Step 1 into adventitious bud induction medium WPM1 for cultivation, the cultivation conditions are: temperature 23-25°C, daily light 12-16h, light intensity 30-40μmol·m -2 ·s -1 Adventitious buds can be induced by culturing for 15-20 days; then continue to be placed in the culture medium WPM1 for the proliferation of adventiti...

specific Embodiment approach 2

[0044] Specific embodiment two: the difference between this embodiment and specific embodiment one is that the specific method of disinfection treatment described in step one is: the tender stems washed with tap water are washed once with sterile water, and then the stems are put into Disinfect in 70% alcohol for 10 sec, take out the stem segment and wash it 3 times with sterile water, then disinfect it with 3% sodium hypochlorite for 40 sec, wash it again with sterile water for 3 times, and finally use sterile filter paper Blot the moisture on the surface of the tender stem, and use sterile scissors to cut off the part of the tender stem morphology that has been oxidized by the disinfectant solution, and it is completed. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0045] Specific embodiment three: the difference between this embodiment and specific embodiment one or two is: the adventitious bud induction medium WPM1 described in the step 2 is made of 400mg / L NH 4 NO 3 , 370mg / L MgSO 4 ·7H 2 O, 990mg / L K 2 SO 4 , 170mg / LKH 2 PO 4 , 96mg / L CaCl 2 2H 2 O, 27.8mg / L FeSO 4 ·7H 2 O, 37.3mg / L Na 2 EDTA, 8.6mg / L ZnSO 4 ·7H 2 O, 0.25mg / L CuSO 4 ·5H 2 O, 22.4mg / L MnSO 4 4H 2 O, 6.2 mg / L H 3 BO 3 , 0.25mg / L Na 2 MoO 4 2H 2 O, 1.0mg / L thiamine hydrochloride, 0.5mg / L pyridoxine hydrochloride, 0.5mg / L niacin, 2.0mg / L glycine, 100mg / L inositol, 25g / L sucrose, 6.5g / L agar, Made from 0.01-1.0mg / L indolebutyric acid, 0.01-1.0mg / L thiadizuron and distilled water, with a pH value of 5.5. Others are the same as in the first or second embodiment.

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Abstract

A method for inducing adventitious buds and plant regeneration of Rhododendron Xing'an relates to the field of plant tissue culture, in particular to a method for inducing adventitious buds and plant regeneration. The purpose is to solve the problem that the existing Xing'an Rhododendron tissue culture organ is difficult and takes a long time. Methods: 1. material collection and processing; 2. adventitious bud differentiation induction and proliferation; 3. adventitious bud elongation induction; 4. adventitious bud rooting culture; 5. seedling hardening and transplanting. In the invention, the hydroponic tender buds of wild plants of Rhododendron Xing'an are used as explants, the induction rate of adventitious buds is as high as 100%, the rooting rate of clump seedlings is as high as 99%, and the survival rate of transplanting is as high as 100%. In about 90 days, starting from the second batch, the culture starts directly from proliferation and differentiation, and it only takes 60 days to reach the seedlings. The present invention is used in the field of plant tissue culture.

Description

technical field [0001] The invention relates to the field of plant tissue culture, in particular to a method for inducing adventitious buds and plant regeneration. Background technique [0002] Rhododendron dauricum L. belongs to Rhododendron dauricum L., Rhododendron dauricum L. Wild Xing'an Rhododendron, also known as Dazixiang, Jindalai, and Yingshanhong, likes light, tolerates half shade, likes cold and humid climates, and is suitable for growing in slightly acidic soil. . In China, it is mainly distributed in Inner Mongolia, Jilin and Heilongjiang's Greater and Lesser Xing'an Mountains, Wanda Mountain, Zhangguangcai Mountain and Laoye Mountain. It is also distributed in northern Korea, Mongolia, Japan, Eastern Siberia and the Far East of the Soviet Union. Xing'an Rhododendron is a rare wild shrub flower. Because wild Xing'an Rhododendron has the characteristics of long flowering period, bright color, and easy survival, Xing'an Rhododendron has been excavated in large...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 李淑娟张含国董实伟董昊白晓明杨宇宁史瀛
Owner 黑龙江岭山农业科技有限公司
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