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Culture medium and sowing method for promoting seed germination of dendrobium huoshanense

A technology of Dendrobium huoshanensis and culture medium, which is applied in the agricultural field, can solve the problems of high cost, low germination rate of Dendrobium huoshanense seeds, and the quality needs to be improved, and achieves the effects of emerald green color, good quality of seed germination, and promotion of germination rate and quality.

Inactive Publication Date: 2019-02-15
安徽祥峰生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem solved by the present invention is that the existing Dendrobium huoshanense seed germination rate is low, the cost is high, and the quality needs to be improved

Method used

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  • Culture medium and sowing method for promoting seed germination of dendrobium huoshanense
  • Culture medium and sowing method for promoting seed germination of dendrobium huoshanense
  • Culture medium and sowing method for promoting seed germination of dendrobium huoshanense

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] A culture medium for promoting seed germination of Dendrobium Huoshanense:

[0035] Contains potassium nitrate 900mg / L, ammonium nitrate 1100mg / L, magnesium sulfate 225mg / L, potassium dihydrogen phosphate 225mg / L, calcium nitrate 180mg / L, potato 50g / L, banana 25g / L, potassium iodide 0.5mg / L, Boric acid 4mg / L, manganese sulfate 20mg / L, zinc sulfate 6mg / L, sodium molybdate 0.1mg / L, copper sulfate 0.02mg / L, cobalt chloride 0.02mg / L, edetate disodium 30mg / L , ferrous sulfate 25mg / L, inositol 80mg / L, glycine 1mg / L, thiamine hydrochloride 0.1mg / L, pyridoxine hydrochloride 0.2mg / L, nicotinic acid 0.2mg / L solid medium;

[0036] Adding an appropriate amount of potatoes can increase the germination rate of the seeds, and adding an appropriate amount of bananas can prevent the albinism of the seeds after germination; figure 1 with figure 2 as shown, figure 1 Pictures of seed germination for medium supplemented with potatoes and bananas, figure 2 It is a picture of seed germi...

Embodiment 2

[0050] A culture medium for promoting seed germination of Dendrobium Huoshanense:

[0051] Contains potassium nitrate 1000mg / L, ammonium nitrate 1200mg / L, magnesium sulfate 250mg / L, potassium dihydrogen phosphate 250mg / L, calcium nitrate 200mg / L, potato 65g / L, banana 35g / L, potassium iodide 0.75mg / L, Boric acid 6mg / L, manganese sulfate 22mg / L, zinc sulfate 8mg / L, sodium molybdate 0.2mg / L, copper sulfate 0.025mg / L, cobalt chloride 0.025mg / L, edetate disodium 35mg / L , ferrous sulfate 28mg / L, inositol 100mg / L, glycine 2mg / L, thiamine hydrochloride 0.5mg / L, pyridoxine hydrochloride 0.4mg / L, niacin 0.4mg / L solid medium.

[0052] A kind of sowing method that promotes the seed germination of Huoshan Dendrobium, comprises the following steps:

[0053] (1) Seed treatment: after the harvested mature, plump and uncracked Dendrobium huoshanense fruit pods were refrigerated at 5°C for 24h, they were taken out and placed at 26°C for 48h;

[0054] (2) Disinfection of seeds: After removing ...

Embodiment 3

[0059] A culture medium for promoting seed germination of Dendrobium Huoshanense:

[0060] Contains potassium nitrate 1100mg / L, ammonium nitrate 1300mg / L, magnesium sulfate 275mg / L, potassium dihydrogen phosphate 275mg / L, calcium nitrate 220mg / L, potato 80g / L, banana 50g / L, potassium iodide 1mg / L, boric acid 7mg / L, manganese sulfate 25mg / L, zinc sulfate 9mg / L, sodium molybdate 0.3mg / L, copper sulfate 0.03mg / L, cobalt chloride 0.03mg / L, edetate disodium 40mg / L, It is made from solid culture medium of ferrous sulfate 30mg / L, inositol 120mg / L, glycine 3mg / L, thiamine hydrochloride 0.2mg / L, pyridoxine hydrochloride 0.6mg / L, niacin 0.6mg / L.

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Abstract

The invention relates to the technical field of agriculture and discloses a culture medium and sowing method for promoting seed germination of dendrobium huoshanense on the basis of existing problemsthat the seed germination rate of dendrobium huoshanense is low, the cost is high, and the quality needs to be improved. The culture medium is a solid culture medium containing 900-1,100 mg / L of potassium nitrate, 1,100-1,300 mg / L of ammonium nitrate, 225-275 mg / L of magnesium sulfate, 225-275 mg / L of potassium dihydrogen phosphate, 180-220 mg / L of calcium nitrate, 50-80 g / L of potatoes and 20-50g / L of bananas. The method for promoting the seed germination includes the steps of (1) seed treatment, (2) seed disinfection, (3) inoculation and (4) culture. The culture medium and the method have the advantages that the seed germination rate is increased from 50-60% to 90% or above, the time required for the seed germination is shortened, and meanwhile the lesion probability of seeds during germination is reduced.

Description

technical field [0001] The invention relates to the field of agricultural technology, in particular to a medium and a method for seed germination of Dendrobium huoshanense. Background technique [0002] Huoshan Dendrobium is a perennial herb of the Orchidaceae Dendrobium genus. It is unique to Huoshan County and has a rare and precious Chinese herbal medicine with geographical indications of origin. Eye-improving function and anti-cancer, anti-aging, anti-radiation and immunity-enhancing effects. [0003] At present, the lack of germplasm resources limits the sustainable utilization of Dendrobium Huoshanense. Dendrobium Huoshanense has a very low natural reproduction rate, a unique living environment, a long growth cycle, and extremely demanding growth conditions. It is extremely difficult to find wild individuals, and its natural resources are almost exhausted. [0004] Using tissue culture technology, seeds can be germinated on artificial medium containing sugar and othe...

Claims

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Application Information

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IPC IPC(8): A01G24/10A01G24/20A01G24/22A01G24/30A01G22/00A01C1/00A01C1/08
CPCA01C1/00A01C1/08A01G22/00A01G24/10A01G24/20A01G24/22A01G24/30
Inventor 高久双兰柳梅王分红刘柳贵戴斯博
Owner 安徽祥峰生物科技有限公司
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