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Method for breeding group seedling-culture of peony and method for preventing it from browning and vetrificating

A tissue culture seedling and vitrification technology, applied in the fields of botanical equipment and methods, horticultural methods, horticulture, etc., can solve the problems of reducing the quality and proliferation rate of seedlings, deformity of tissue culture seedlings, and reducing the proliferation rate of tissue culture seedlings.

Inactive Publication Date: 2006-03-08
BEIJING FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to reports, the proliferation rate of various species in peony tissue culture is generally low, generally around 3.0, which means that the proliferation rate needs to be further improved; at the same time, the vitrification of tissue cultured seedlings leads to deformity and sickness of tissue cultured seedlings. The quality and proliferation rate of seedlings are reduced; and the occurrence of browning is toxic to tissue culture seedlings, leading to death, and also reduces the proliferation rate of tissue culture seedlings

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1 Proliferation

[0020] Medium: with MS (Ca 2+ Doubling) is basic medium, sucrose 40g / L, agar 6g / L, pH value 5.8, additional hormone is:

[0021] 'Noon': BAP1.0mg / L, GA 3 0.2mg / L;

[0022] 'Oolong Pengsheng': BAP0.5mg / L, GA 3 0.5mg / L;

[0023] 'Star': BAP0.5mg / L, GA 3 1.0mg / L;

[0024] 'Coral Table': BAP1.0mg / L, GA 3 0.5mg / L;

[0025] ‘Zi Erqiao’ BAP1.0mg / L, GA 3 0.5mg / L.

[0026] Use the dormant buds that are about to germinate in early spring as explants, peel off the bud scales after disinfection and sterilization, cut off the small leaves in the buds, inoculate the remaining parts on the culture medium, observe and count every 7 days, and record the growth of various varieties. Proliferation rate and growth status on different media. Subculture after 5 weeks of culture, the method is to cut off the leaves from the base of the petiole of the tissue cultured seedlings, then cut longitudinally along the central axis of the stem, cut across at 0.5 cm...

Embodiment 2

[0030] Example 2 Prevent Vitrification

[0031] Keep the contents of other elements in the MS basic medium unchanged (Ca 2+ double), 1 / 2 times macroelements (CaCl 2 with Ca(NO 3 ) 2 The mol ratio is 1: 1), sucrose 40g / L, agar 6g / L, pH5.8, the hormone concentration of each kind adopts the concentration among the embodiment 1.

[0032] Use the dormant buds that are about to germinate in early spring as explants, peel off the bud scales after sterilization, cut off the small leaves in the buds, and inoculate the remaining parts on the medium for cultivation. Subculture after 5 weeks of culture, the method is to cut off the leaves from the base of the petiole of the tissue cultured seedlings, then cut longitudinally along the central axis of the stem, cut across at 0.5 cm below the position where new shoots occur, and inoculate the obtained part in the new stem. Cultured on the culture medium (subsequent subculture process is the same), a total of 2 subcultures were carried ou...

Embodiment 3

[0035] Embodiment 3 prevents browning,

[0036] The hormone concentration of each kind adopts the concentration of embodiment 1, sucrose 40g / L, agar 6g / L, pH5.8.

[0037] 'Oolong Pengsheng': improved MS medium, 1 / 2 trace elements, Ca 2+ Doubled, CaCl 2 with Ca(NO 3 ) 2 The molar ratio is 1:1.

[0038] 'Star': Improved MS medium, 1 / 3 trace elements, Ca 2+ Doubled, CaCl 2 with Ca(NO 3 ) 2 The molar ratio is 1:1.

[0039] Take the dormant buds that are about to germinate in early spring as explants, peel off the bud scales after disinfection and sterilization, cut off the small leaves in the buds, inoculate the remaining parts on the culture medium, and observe and record the growth of various varieties in different cultures every 7 days. base level of browning. Subculture after 5 weeks of culture, the method is to cut off the leaves from the base of the petiole of the tissue cultured seedlings, then longitudinally cut along the central axis of the stem, and cut transve...

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Abstract

The present invention uses the dormand bud to be activated in early spring as explant and adopts the following steps: sterilizing, removing bud scale, cutting off small leaflet in the bud, inoculating residual portion on the different culture media to make culture, then regularly making subculture so as to implement propagation of lots of tissue-cultured seedlings and prevent the production of browning and vitrifiation.

Description

technical field [0001] The invention relates to plant seedling propagation technology, in particular to a method for preventing the proliferation, browning and vitrification of tree peony tissue culture seedlings. Background technique [0002] Peony is a traditional famous flower of our country's special products. Our people have always regarded peony as a symbol of prosperity, prosperity, peace and happiness of the Chinese nation. In recent years, peonies have become more and more widely used in beautifying the environment, commodity production, and social culture. This needs to be based on a large number of peony seedlings. However, only single-petal varieties of peonies are more sturdy, followed by semi-double-petal varieties and double-petal varieties. Generally, it is not strong, and the seedlings are highly variable, and it is not easy to maintain the excellent traits of the mother plant. In addition, traditional propagation methods such as grafting and division, as w...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 成仿云李萍
Owner BEIJING FORESTRY UNIVERSITY
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