Method for multiplying cymbidium hybridum seedlings effectively by utilizing shoot tip tissues

Abstract

The invention provides a method for multiplying cymbidium hybridum seedlings effectively by utilizing shoot tip tissues. The method comprises the following steps: selection of explants; lateral bud disinfection; protocorm inducing culture; protocorm multiplication culture; protocorm differentiation culture and rooting and hardening-off culture. The method has the advantages that the inductivity of protocorms is improved in the inducing stage; the aberration rate and the vitrification ratio of the protocorms are reduced in the multiplication stage; the bud variation ratio is reduced in the differentiation stage; in the rooting and hardening-off stage, the growth and the development of the seedlings are better, the twisting ratio of new roots is low, the roots are more disperse and stretch out better, and the seedling aberration rate is reduced.

Description

technical field [0001] The invention relates to a method for efficiently propagating Cymbidium cymbidium seedlings by using stem tip tissue, in particular to a tissue culture method that can make the growth and development of the seedlings more robust, reduce the tangle rate of new roots, and reduce the variation rate of the seedlings. Background technique [0002] Cymbidium hubridum (Cymbidium hubridum) is a group of fine varieties with large flowers, bright colors and robust growth, which are bred through multi-generation hybrid selection of some large-flowered epiphytic species in the genus Orchid. Cymbidium grandiflorum has beautiful posture, long green leaves, rough flower posture, and the flowering period is as long as 2-3 months. It is a very important indoor ornamental plant and a world-renowned "orchid star". It is rich and colorful, very popular in the international flower market, and deeply loved by people all over the world. [0003] At present, in production, t...

AI Technical Summary

Problems solved by technology

[0004] First, when the shoot tips of lateral buds are used for propagation, if the shoot tip tissue is too large, the disinfection is often incomplete, and the success rate of disinfection is extremely low. At the same time, the shoot tip tissue absorbs nutrients slowly, and it is easy to brown during cultivation

If the shoot tip tissue is too small, the viability will be greatly reduced, and it will easily die in the later stage of culture, resulting in a low protocorm induction rate

[0005] The second is that the protocorm of Cymbidium is very easy to mutate in the multiplication stage, and the vitrification phenomenon is more serious. It is difficult for this protocorm to differentiate buds in the later stage.

[0006] The third is that some protocorms will gradually become rigid during the differentiation stage of Cymbidium cymbidium. The main manifestation is that the pseudobulbs at the base will be abnormally hypertrophic, and the leaves will not be differentiated, or even if the leaves can be differentiated, the leaves will be small and cannot grow further, leading to the differentiation of the protocorms. Bud variation of the

[0007] The fourth is that the seedling variation rate of Cymbidium grandiflora is relatively high in the stage of rooting and strong seedlings, and the seedlings are not strong enough, so there will be many different types of mutant seedlings, such as cross seedlings with alternate or clustered leaves, and long internodes. Bamboo seedlings

In addition, the new roots of seedlings have a high rate of entanglement and are not easy to separate, which is not conducive to later greenhouse transplanting and domestication