Production method of adiantum
A production method, the technology of Adiantum fern, applied in the field of plant tissue culture, can solve the problems of lower spore germination rate, sporophyte induction rate, unstable light and humidity in greenhouses, and difficult control of spore seeding density, so as to reduce production costs, Saving manpower and easy operation
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[0019] The present invention will be further described below in conjunction with specific embodiments.
[0020] First, prepare M1 medium: Weigh the following raw materials according to 1 / 2 of the normal amount of MS: NH 4 NO 3 , KNO 3 , MgSO 4 ·7H 2 O. KH 2 PO 4 ; Other components are weighed according to the normal amount of MS medium, then add 2% white sugar, 0.6% agar, and dilute to volume with distilled water, then adjust the pH of the solution to 5.8-6.0 with HCL or NaOH, heat to melt and divide into packs After sterilization at high temperature and high pressure for 12-15min, it becomes M1 medium;
[0021] Next, spore germination: take the sporophyte containing brown mature sporangia as explants, and disinfect the surface of the explants as follows: first rinse the surface of the sporophyte with running water for 30 minutes, and put the sporophylls in the laboratory. Cut off the petioles one by one, then place the sporophylls in 0.1% HgCl containing a few drops of...
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