Formula of tissue culture medium for production of American red maple saplings and culture method

Abstract

The invention discloses a formula of a tissue culture medium for production of American red maple saplings and a culture method. The formula has the advantages that by improving on the basis of an MS culture medium, a red maple culture medium (HF) is invented; the American red maple is subjected to tissue culture by 25g/L of saccharose, 7g/L of agar and 0.010mg/L to 0.003mg/L of phenyl urea derivative (TDZ), so that the propagation speed of the American red maple is greatly accelerated; the propagation expanding coefficient of the American red maple is improved; after culturing for six weeks, the propagation expanding coefficient can increased by two times and reaches 6.5; the yearly output of the American red maple is increased, and the output and economic benefits are large.

Description

technical field [0001] The invention relates to a formula and a culture method of a tissue culture medium for the production of American red maple seedlings [0002] , belonging to the field of red maple cultivation technology. Background technique [0003] The invention discloses a method for tissue culture and rapid propagation of American red maple. The American red maple is a tall deciduous tree belonging to the Aceraceae family and has the characteristics of strong adaptability, drought resistance, temperature resistance, rapid growth, etc., and is an excellent landscaping method. species. [0004] At present, American red maple is mainly propagated by seed sowing, but because of the low seed setting rate, tissue culture is required. Now most of the tissue culture of American red maple is based on improved MS medium (MS medium in MS medium) Ammonium nitrate halved) as the basal medium, the specific formula is: improved MS+25g / L sucrose, 7g / L agar, 0.5mg / L cytokinin, 0...

AI Technical Summary

Problems solved by technology

[0004] At present, American red maple is mainly propagated by seed sowing, but because of the low seed setting rate, tissue culture is required. Now most of the tissue culture of American red maple is based on improved MS medium (MS medium in MS medium) Ammonium nitrate halved) as the basal medium, the specific formula is: improved MS+25g / L sucrose, 7g / L agar, 0.5mg / L cytokinin, 0.01mg / L indolebutyric acid or naphthaleneacetic acid, but, The multiplication coefficient of this formula is not high in the process of subculture, only about 3.0; a bud explant takes 3 and 6 as the multiplication coefficient respectively, for example, if the multiplication coefficient is 3, Then the annual production capacity is 6,600 plants, and if the multiplication coefficient is 6, then the annual production capacity is 1,679,600 plants. This is an extremely huge gap between production capacity and economic benefits. Tissue culture medium with multiplication factor to improve the production capacity and economic benefits of American red maple

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