Method for cultivating polygala fallax hemsl tissue culture seedlings based on multiple internodal stem segments integration

A technology of yellow-flowered inverted water lilies and tissue culture seedlings, which is applied in the field of plant cultivation, can solve the problems of low reproduction multiples, long reproduction time, and high seedling multiples, and achieve the effects of reducing production costs and increasing reproduction multiples

Active Publication Date: 2015-10-28
SANMING UNIV
View PDF2 Cites 10 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Existing propagation techniques such as seed propagation and cutting propagation all have disadvantages such as too long propagation time and low multiplication factor.
The existing tissue culture technology uses a single stem segment to induce single buds, then uses single buds to induce clustered buds, and finally uses clustered buds to induce seedlings. The multi-process and multi-step method (Lin Wenge, 2012) makes the tissue culture seedlings of Nymphaea chinensis The whole process of cultivation is too long, and the reproduction coefficient is too large, resulting in too many generations of seedlings and a sharp drop in quality, which increases the variability of yellow-flowered water lily seedlings

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] (1) Surface disinfection: Take the wild Nymphaea japonica plant that grows vigorously and has no damage by diseases and insect pests as explants, remove the leaves and lightly brush the epidermis with a soft brush, carry out surface disinfection treatment a, and cut into 3 multi-sections The stem segments in between are reserved;

[0049] (2) Primary culture - cluster bud induction and proliferation: Inoculate the sterilized multi-node stem segments into medium A, lay the multi-node stem segments horizontally in the medium, and carry out integrated culture after 15 days ;

[0050] (3) Integrated culture: Cut the multi-node clustered buds induced by the first generation into 2.0 cm long single buds and put them horizontally in the medium B, inoculate 30 plants in each bottle, and the light intensity is 3000 lx, and start to take root after 10 days. After 20 days, grow to a height of 8 cm and prepare for transplanting;

[0051] (4) Seedling hardening: After 20 days,...

Embodiment 2

[0060] (1) If the Nymphaea chrysalis tissue culture seedlings with strong growth are selected and obtained as the female parent of the explants for propagation, the surface disinfection should be processed by disinfection treatment b, and then the primary culture is carried out after directly cutting out;

[0061] (2) Primary culture - cluster bud induction and proliferation: Inoculate the sterilized multi-node stem segments into medium A, lay the multi-node stem segments horizontally in the medium, and carry out integrated culture after 12 days ;

[0062] (3) Integrated culture: cut the multi-node clustered buds induced by the first generation into 1.5 cm long single buds and put them horizontally in the medium B, inoculate 30 plants in each bottle, the light intensity is 2000 lx, and start to take root after 10 days. After growing to a height of 6 cm in 20 days, prepare for transplanting;

[0063] (4) Seedling hardening: After 20 days, take the bottle seedlings out of t...

Embodiment 3

[0072] (1) Surface disinfection: Take the wild Nymphaea chinensis plant that grows vigorously and has no damage by diseases and insect pests as explants, remove the leaves and lightly brush the epidermis with a soft brush, carry out surface disinfection treatment a, cut into 4 multi-sections The stem segments in between are reserved;

[0073] (2) Primary culture - cluster bud induction and proliferation: Inoculate the sterilized multi-node stem segments into medium A, lay the multi-node stem segments horizontally in the medium, and carry out integrated culture after 10 days ;

[0074] (3) Integrated culture: cut the multi-node clustered buds induced by the first generation into 2.0 cm long single buds and put them horizontally in the medium B, inoculate 30 plants in each bottle, the light intensity is 1500 lx, and start to take root after 10 days. After growing to a height of 7 cm in 20 days, it is ready to be transplanted;

[0075] (4) Seedling hardening: After 20 days,...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a method for cultivating polygala fallax hemsl tissue culture seedlings based on multiple internodal stem segments integration. The method comprises the following steps: using of polygala fallax hemsl plants as explants, surface sterilization, primary culture, integrated cultivation, seedling hardening and transplantation. Thus, the polygala fallax hemsl tissue culture seedlings are finally obtained. According to the invention, multiple internodal stem segments are used as explants, the multiple internodal stem segments are put in a primary culture medium according to an independent formula from special studies to finish proliferation, rooting and other processes, and the polygala fallax hemsl tissue culture seedlings are cultivated by an integration method. By the method, propagation coefficient is raised, and high quality of nursery stock is maintained to meet introduction and popularization requirements. By the polygala fallax hemsl tissue culture technology, a lot of polygala fallax hemsl plants with excellent quality of female parents can be propagated within a short period of 30-50 d.

Description

technical field [0001] The invention belongs to the field of plant cultivation, and in particular relates to a method for cultivating tissue-cultured seedlings of Nymphaea japonica based on the integration of multi-node stem segments. Background technique [0002] Yellow flower inverted water lily ( Polygala fallax Hemsl.) Also known as yellow ginseng, chicken tree, hanging yellow, yellow flower hanging water lotus, Guanyin string, yellow flower Dagala, Polygalaceae (Polygalaceae) Polygalaceae ( Polygala )plant. It is mainly distributed in Guangxi, Fujian, Hunan and other places. It grows in hillside sparse forests or valley jungles. It is a precious Chinese herbal medicine. The root or whole plant of the yellow water lily is a folk medicine commonly used by ethnic minorities such as Yao, Miao, and Zhuang. It has the effects of tonifying, strengthening, dispelling dampness, and relieving fatigue. hit damage. In recent years, the medicinal value of Nymphaea chinensis ha...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 张君诚张杭颖
Owner SANMING UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap