Potato stem fragment tissue culture medium and culture method thereof

The technology of a tissue culture medium and a culture method is applied in the field of one-step seedling culture of potato test-tube seedlings and stems, and the field of tissue culture medium of potato stems. The effect of short cycle, reduced economic cost and simple training procedure

Inactive Publication Date: 2017-08-18
甘肃省农业科学院马铃薯研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The main purpose of the present invention is to provide a kind of one-step

Method used

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  • Potato stem fragment tissue culture medium and culture method thereof
  • Potato stem fragment tissue culture medium and culture method thereof
  • Potato stem fragment tissue culture medium and culture method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 2

[0061] Utilize described potato one-step seedling-forming medium, make potato stem section tissue culture one-step seedling, comprise the following steps: (1), add 1mg 6-benzylaminopurine, 0.01mg naphthaleneacetic acid, 2.5 to every liter of MS medium mg gibberellin, 50 mg inositol, 25 g edible sugar, and 3 g agar; the pH value was adjusted to 5.8; after aliquoting, it was sterilized by high-pressure steam. Put the sterilized culture medium into the inoculation chamber and let it cool down to solidify.

[0062] (2) Select aseptic test-tube seedlings of the Atlantic potato variety with a seedling age of 20-30 days, cut 0.5-1 cm stem segments without axillary buds under sterile conditions, and inoculate them on the above-mentioned medium. Place the inoculated culture bottle on a lighting culture rack for cultivation, the temperature is 21±2°C, the light intensity is 3500LX, and the light time is 16h / d.

[0063] (3) Cultivate for 28 days to obtain regenerated potato plants.

Embodiment 3

[0065] Add 3mg 6-benzylaminopurine, 0.1mg naphthaleneacetic acid, 7.5mg gibberellin, 100mg inositol, 30g edible sugar and 4.5g agar to each liter of MS medium; After the agar is completely dissolved, adjust the pH value to 6.0; after dispensing, high-pressure steam sterilization, steam temperature 120 ° C, pressure 1.5Mpa conditions for 15 to 20 minutes, the sterilized medium is left to cool down and solidify; under sterile conditions , cut 0.5cm ~ 1cm stem section without axillary buds, and inoculate it in the medium of step B; place the medium inoculated with the stem section in step C under the conditions of a temperature of 21±2°C, a light intensity of 4000LX, and a light time of 16h / day Under cultivation; the potato variety LK99 with a seedling age of 20 to 30 days was selected, and the one-step seedling growth medium was prepared and cultivated in the same way as above, and cultivated for 40 days to obtain potato regenerated plants.

Embodiment 4

[0067] Add 3mg 6-benzylaminopurine, 0.01mg naphthaleneacetic acid, 5mg gibberellin, 100mg inositol, 30g edible sugar and 4.5g agar to each liter of MS medium; After complete dissolution, adjust the pH value to 6.0; after subpackaging, high-pressure steam sterilization, steam temperature 120 ° C, pressure 1.5Mpa conditions for 15 to 20 minutes, the sterilized culture medium is cooled and solidified; under sterile conditions, cut out 0.5cm ~ 1cm stem section without axillary buds, inoculated in the medium of step B; the medium inoculated with the stem section of step C was cultured under the conditions of temperature 21±2°C, light intensity 4000LX, and light time 16h / day ;

[0068] The potato variety Longshu No. 7 with a seedling age of 20-30 days was selected, and the one-step seedling growth medium was prepared and cultured in the same way as above, and cultured for 32 days to obtain potato regenerated plants.

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Abstract

The invention relates to the technical field of plant tissue culture, in particular relates to a potato stem fragment tissue culture medium, and a one-step seedling culture method for potato test-tube plantlet stem fragments. An MS traditional culture medium is selected to provide inorganic nutritional elements and partial organic nutritional elements required by tissue growth and development. The culture medium provided by the invention has a broad spectrum, and is suitable for one-step seedling culture for stem tissues of most potato varieties. Through the reasonable control of the use and compounding ratio of hormones and the addition of other auxiliary materials, the stem fragment explant does not need to be transferred to a differential culture medium after forming callus tissues, instead, is subjected to direct differentiation and seedling in an original culture medium, so as to shorten the culture time by about two months and reduce the economic cost. In addition, the culture medium has a broad spectrum, and is suitable for most varieties.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a potato stem section tissue culture medium, and also includes a one-step seedling cultivation method for the potato tube seedling stem section. Background technique [0002] Potato (Solanum Tuberosum L.) is an annual herbaceous tuber plant of the family Solanaceae. It is an important grain, vegetable, feed and industrial raw material and crop, and occupies a very important position in my country's agricultural production. In order to innovate germplasm resources, tissue culture technology has been widely used in potato breeding research, and has achieved remarkable results. [0003] In recent years, the rapid development of biotechnology has brought vitality to the research and production of potato. The genetic improvement of potato through tissue culture and genetic transformation has become an important aspect of potato molecular breeding. However, whether it is ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 齐恩芳贾小霞刘石吕和平黄伟张荣
Owner 甘肃省农业科学院马铃薯研究所
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