Method for rapidly obtaining pure line of hybrid wheat
A fast technology for hybrid wheat, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems that restrict the development and application of haploid breeding technology, low frequency of microspore embryo induction, etc.
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Embodiment 1
[0049] Example 1 A method for quickly obtaining pure lines of hybrid wheat includes the following steps:
[0050] ⑴Prepare hybrid combination:
[0051] According to the breeding goal, select the varieties with complementary traits as parents, artificially emasculate and pollinate, and after maturity, the hybrid ears are harvested according to the hybrid combination, and the hybrid ears are individually threshed to obtain the hybrid combination material seeds and save them for next year's sowing.
[0052] ⑵ Planting hybrid materials:
[0053] During the wheat sowing period, sow 60 seeds of hybrid combination materials in the field at 20cm row spacing and 10cm plant spacing. After sowing, use a rake to level the field along the sowing ditch. In the field management, attention should be paid to pest control and disease treatment to ensure the healthy growth of hybrid materials.
[0054] ⑶Determine the appropriate ear picking period:
[0055] Take the anthers in the middle of the wheat...
Embodiment 2
[0095] Example 2 A method for quickly obtaining pure lines of hybrid wheat includes the following steps:
[0096] ⑴Prepare hybrid combinations; ⑵Plant hybrid materials; ⑶Make sure that the appropriate ear picking periods are the same Example 1 .
[0097] ⑷Pretreatment of anthers:
[0098] First, the wheat ears obtained in step (3) are surface-sterilized with alcohol with a mass concentration of 75%, and then put on a sterile ultra-clean workbench. After the alcohol on the surface is evaporated, the wheat ears are peeled off; then the wheat ears are placed Put it into a sterile Erlenmeyer flask, add 0.1% HgCl solution with a mass concentration of 0.1%, and shake it for 4 minutes, pour out the HgCl solution, wash the wheat ears with sterile distilled water three times, take out the wheat ears, and absorb the surface moisture with sterile filter paper , Take out the anthers with sterile tweezers; finally inoculate the anthers in an Erlenmeyer flask containing 3ml of pretreatment ...
Embodiment 3
[0116] Example 3 A method for quickly obtaining pure lines of hybrid wheat includes the following steps:
[0117] ⑴Prepare hybrid combinations; ⑵Plant hybrid materials; ⑶Make sure that the appropriate ear picking periods are the same Example 1 .
[0118] ⑷Pretreatment of anthers:
[0119] First, the wheat ears obtained in step (3) are surface-sterilized with alcohol with a mass concentration of 75%, and then put on a sterile ultra-clean workbench. After the alcohol on the surface is evaporated, the wheat ears are peeled off; then the wheat ears are placed Put it into a sterile Erlenmeyer flask, add 0.1% HgCl solution with a mass concentration of 0.1%, and shake it continuously for 5 minutes, pour out the HgCl solution, wash the wheat ears with sterile distilled water three times, take out the wheat ears, and absorb the surface moisture with sterile filter paper , Take out the anthers with sterile tweezers; finally, inoculate the anthers in an Erlenmeyer flask containing 3ml of...
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