Culture medium for improving protoplast regeneration frequency of lupinus albus

Abstract

The invention relates to a culture medium for improving the protoplast regeneration frequency of lupinus albus, and the method comprises the following components: a minimal medium, hormone and other components, wherein the minimal medium is composed of mass and trace of B5 and organic MS; the hormone is combined as follows: 0.5 mg / L NAA (Naphthyl Acetic Acid) + 2.0 mg / L 6-BA (Butyl Acrylate) + 1.0 mg / L TDZ (Thidiazuron) + 0.5 mg / L gibberellins; and the other components are combined as follows: 5 g / L cane sugar + 126 mg / L fructose + 120 mg / L ribose + 120 mg / L xylose + 126 mg / L mannose + 137 mg / L cellobiose + 200 mg / L casein acid hydrolysate +5 g / L silver nitrate + 8 g / L agarose. The culture medium can be used for solving the bottleneck problem of growing a lupinus albus callus into a plant, and has great significance to developing biotechnology related to lupinus albus.

Description

technical field [0001] The invention relates to a culture medium for increasing the regeneration frequency of lupine protoplasts, which belongs to the field of modern agriculture and plant biotechnology research. Background technique [0002] Lupine is a herbaceous plant with extremely high application value. Its seed protein content is as high as 50%, and its oil content is 5%-20%. It can not only be used as food, but also as high-quality feed. At the same time, the flower shape of lupine is beautiful, and the flowering period is more than 2 months. It has single color and multiple colors. It is very decorative when planted indoors, in courtyards, flower beds and other places. At present, it is planted in many countries in Europe, Australia, Asia and Africa. The widely cultivated varieties mainly include white lupine (Lupinus albus), narrow-leaved lupine (L. angustifolius) and yellow lupine (L. luteus). my country's lupine is limited to sporadic cultivation attempts, mainl...

AI Technical Summary

Problems solved by technology

[0003] Lupine is an important economic plant, but its regenerative ability is very poor, not only from callus to regenerated plant, but also from plant to root. It is currently recognized in the world as a plant that is difficult to carry out biotechnology research through tissue culture methods one

At present, there is only one report about lupine protoplast regenerated plants at home and abroad, and only one regenerated plant was obtained. Therefore, the development of its biotechnology is limited, including protoplast fusion, embryo culture and microspore culture, etc.; Facilitating lupine-related biotechnological research

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