Method for improving tissue culture regeneration rate of wheat genotype immature embryo with low regeneration capacity

Abstract

The invention discloses a method for improving a tissue culture regeneration rate of a wheat genotype immature embryo with low regeneration capacity, which comprises the following steps that 1) an immature embryo of a wheat variety with high regeneration capacity and an immature embryo of a wheat variety with low regeneration capacity are inoculated inside the same container with a callus induction culture medium at line intervals to be cultured in an induction way under a darkness condition, so that a callus of the wheat variety with the high regeneration capacity and a callus of the wheat variety with the low regeneration capacity are obtained; and 2) the callus of the wheat variety with the high regeneration capacity and the callus of the wheat variety with the low regeneration capacity are transferred into the same culture container with a differential culture medium at line intervals to be differentially cultured under the illumination condition, so that a regeneration plant of the wheat variety with the low regeneration capacity and a regeneration plant of the wheat variety with the high regeneration capacity are obtained. Due to the adoption of the method, a regeneration rate, an embryo callus induction rate and a callus differentiation rate of the plants cultured by the immature embryos of the wheat variety with the low regeneration capacity can be remarkably improved.

Description

technical field [0001] The invention relates to a method for improving the regeneration rate of low regenerative wheat genotype immature embryo tissue culture. Background technique [0002] The high-frequency plant regeneration of wheat tissue culture in vitro is one of the important links in the development of wheat cell engineering breeding and transgenic breeding. So far, regenerated plants have been obtained by culturing explant materials such as wheat immature embryos, young spikes, anthers, mature embryos, and microspores. Among them, wheat immature embryos are considered to be the explant type with the highest regeneration rate. However, there are significant differences among different genotypes in wheat immature embryo culture, and the genotypes that can obtain regenerated plants at a high frequency are very limited, especially for some fine wheat varieties that are popularized in a large area, the regeneration ability of immature embryos is often very low. The ada...

AI Technical Summary

Problems solved by technology

Studies have shown that technical strategies such as improved medium (such as SD2 medium) and culture procedures (such as intermittent auxin treatment) can increase the regeneration rate of immature embryos of wheat varieties with medium or higher cultivation ability, but the cultivation of immature embryos of wheat varieties with weak regenerative ability The effect is not significant

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