A populus trichocarpa Torr.Gray leaf adventitious bud induction and plant regeneration method aims to solve the problems that at present, populus trichocarpa Torr.Gray has difficulty in tissue culture organogenesis and low in plant regeneration rate, and the adventitious bud inductivity is low and the period is long since populus trichocarpa Torr.Gray stems are taken as explants. The method provided by the invention comprises the following steps: 1, material collection and treatment; 2, inoculated culture and aseptic seedling expanding propagation: stems which are flushed by tap water are subject to sterile treatment for inoculated culture to propagate aseptic seedlings; 3, adventitious bud differentiation induction; 4, adventitious bud elongation induction: calluses with adventitious buds are cut into blocks and placed in an adventitious bud elongation culture medium for culture; 5, adventitious bud rootage culture; 6, hardening-seedling and transplanting. According to the invention, populus trichocarpa Torr.Gray aseptic seedling leaves are taken as explants, so that the adventitious bud inductivity and the survival rate are up to 100%, and from explant culture to seedling survival, only about 60 days are required. The populus trichocarpa Torr.Gray leaf adventitious bud induction and plant regeneration method is used for plant tissue culture.