54 results about "Plant Organogenesis" patented technology

The invention relates to a method using the cotyledon node of soybeans as explant to perform soybean organogenesis and plant regeneration, belonging to the technical field of agricultural production. The invention is characterized in that based on the traditional tissue culture, the cotyledon node of soybeans is used as explant to perfect the regeneration system of organogenesis means. The invention has the advantages that based on the selection of effective disinfection technology for soybean seeds, the improvement and the optimization of various medium compositions is outstanding; through the verification of the tissue culture of conventional soybean varieties, the induction, the elongation and the rooting of multiple shoot are effectively promoted; the quality of aseptic seedlings and the differentiation rate of the multiple shoot are improved; the organogenesis and plant regeneration process of the cotyledon node of majority varieties are perfectly realized; solid technical support is provided for the genetic transformation and the molecular improvement of soybeans; and the invention has important practical significance to the effective breeding and health production of the soybeans in China.

The present invention is directed to methods for enhancing the replicative capacity of cells, by culturing the cells in the presence of an active agent or compound which inhibits SIRT1. One method provides expanding stem cells by culturing the cells in the presence of a SIRT1 inhibitor. The resulting cultured cells can be used for a variety of applications including cell-based therapies such as bone marrow transplants, gene therapies, tissue engineering, and in vitro organogenesis.

A method for in vitro culturing an adventitious bud by a Korean larch to induce a plant regeneration relates to a method for in vitro culturing the adventitious bud by pine to induce the plant regeneration which solves the problems of difficult generation of the tissue culture organs of the Korean larch, low plant regeneration rate and low inducing rate by using a mature embryo, a flower bud and axillary bud, as well as a tender stem segment of the Korean larch as an explant adventitious bud currently. The method relates to: 1. Culturing and inducing a callus by a zygotic embryo; 2. Inducing to generate the adventitious bud; 3. Continuously culturing and inducing to root, namely acquiring the regeneration plant in vitro of the Korean larch. The invention induces the adventitious bud by taking a mature seed of the Korean larch as the explant and acquires the adventitious bud of high inducing rate which is improved by more than 9 times than the currently existing adventitious bud inducing technology and the inducing rate of the callus is all higher than 90 percent, which has a wide application value on the massive expanding propagation and gene engineering breading research of the Korean larch.

A method assay for rapidly and reproducibly generating hair follicles from dissociated epithelial and mesenchymal cells is disclosed. The method serves both as a tool for measuring the trichogenic (i.e., hair growth-inducing) property of cells and for studying the mechanisms dissociated cells employ to assemble an organ. In a method of this application dissociated cells, isolated from newborn mouse skin, are injected into adult mouse truncal skin, hair follicles develop. This process involves the aggregation of epithelial cells to form clusters which are sculpted by apoptosis to generate “infundibular cysts”. From the “infundibular cysts” hair germs form followed by follicular buds and then pegs which grow asymmetrically to differentiate into cycling mature pilosebaceous structures. Using various techniques, exposure of the “infundibular cysts” by puncturing, piercing, or scratching the skin and, in an approach, covering the exposed cysts with a wound dressing material produced egressing hair follicles.

Formation of nitrogen fixing root nodules in legumes is induced by perception of lipochitin-oligosaccharide signal molecules secreted by compatible Rhizobium bacteria, which triggers a common symbiotic pathway. The present invention provides a spontaneous nodule formation (snf2) mutant, in which the formation of symbiotic nodules is spontaneous, leading to nodule development in the absence as well as in the presence of Rhizobium bacteria and / or exogenous rhizobial signals. The invention further provides an isolated DNA sequence encoding a mutant cytokinin-independent histidine kinase whose activity results in this ‘gain of function’ dominant phenotype of spontaneous nodule formation. Furthermore the snf2 gene is shown to confer a phenotype, characterised by regulated organogenesis of spontaneous nodules, to plants having a nodulation deficient genetic background. A gene of the invention, that confers this spontaneous nodulation phenotype, has 15 utility for the transfer and establishment of nitrogen fixing capability in non-nodulating plants, and thereby reducing the nitrogen fertiliser dependence of non-nodulating crop plants.

The invention discloses a method for inducing hybrid larch plant regeneration through in vitro culture of adventitious buds, relates to a method for inducing pine plant regeneration through in vitro culture of adventitious buds, and aims at solving the problems that in the existing tissue culture method of hybrid larch, organogenesis is difficult, plant regeneration rate is low, and adventitious bud induction rate is low. The method comprises the following steps: 1 inoculating pretreated zygotic embryos of the hybrid larch into a culture medium, and carrying out dark cultivation or light cultivation until callus tissue is generated; 2 inoculating the callus tissue into the culture medium, carrying out light cultivation until an adventitious buds grow; 3 cutting the callus tissue with the adventitious buds into blocks, transferring into the culture medium, carrying out subculture for once after 3 weeks, carrying out adventitious bud elongation, transferring into the culture medium, and carrying out stooling of the adventitious buds; and 4 transferring the stooled adventitious buds into the culture medium, and carrying out light cultivation to realize rooting of the adventitious buds. According to the method disclosed by the invention, the induction rate of the adventitious buds is 87.73%; the stooling rate is 75.96%; the rooting rate is 45%; and the method is applied for inducing hybrid larch plant regeneration through in vitro culture of adventitious buds.

The invention discloses a succeeding preservation method for embryogenic callus and a subculture medium used therein. The succeeding preservation method for embryogenic callus is to subculture embryogenic callus with the subculture medium. The succeeding preservation method for embryogenic callus and the subculture medium provided in the invention has a good culture effect on embryogenic callus of China fir, enable embryogenic competence of callus of China fir to be maintained for a long time, provide great convenience to subsequent research on somatic embryogenesis, organogenesis and the like of China fir and have a great practical application value.

The invention provides for a device, system, and methods for using the same with kidney progenitor cells, specifically ureteric bud (UB) cells, metanephric mesenchymal (MM) cells, and the stromal cell (SC) subpopulation of the metanephric mesenchyme, to generate an embryonic kidney organoid that can be implanted into a mammalian subject to create a living, functional kidney.

The invention relates to a quick adventitious buds inducing method for the panax tuber section. The method uses the panax tuber section as the explants, and induces the adventitious buds on the MS substrate of the additional hormone, and to proceed with the multiplication of the adventitious buds. The invention can directly induce the buds through the path produced by the direct organs, the speed is high, the sprouting rate is high, the cost is low, and the inducing process of the adventitious buds can be completed after 25 days about, the reproducing frequency is high, the variation rate is low, the period is relatively short, and the un-differentiated cells can directly differentiate into buds, so that the clone variation of the body cells is small. The invention can better keep the genetic stability of the host plant, and can ensure the transformed exogenous gene to transmit stably, the limit from the genotype is low, the adventitious buds obtaining rate is relatively high, and thereby is an effective measurement for obtaining the artificially cultivated panax seedlings in large scale.

The invention relates to a conjoined grafting method for plant meristem. The conjoined grafting method for the plant meristem is characterized in that the conjoined grafting method through meristem between different phylum, klasse, order, family, genus and species of plantae is provided, and the conjoined grafting method comprises an apcial meristem, a lateral meristem and an intercalary meristem. The meristem possesses massive reversible gene regulatory networks and spatial and temporal expression opportunities due to the fact that the meristem has high cell division ability and is a key tissue of organogenesis and morphogenesis and a starting point of organ and tissue differentiation. Therefore, conducting moderate manual injury intervention and conjoined grafting on the meristem can greatly increase opportunity of successful grafting, and enlarges boundary capable of being conducted grafting between different phylum, klasse, order, family, genus and species of the plantae. Due to the fact that scion and rootstock whole plant are not separated, bud mutation and fructification are promoted to obtain grafting progeny seeds, exchanging and changing of part genetic materials of grafting progeny can be achieved, and consequently, low coat and safe material exchanging of extra distant species of the plantae can be achieved, variation is induced, and then a new germplasm of a scion plant is created.

The invention relates to an early blossoming regulating and controlling method for potted osmanthus fragrans. The regulation and control method is characterized by comprising the following steps of: 1, promoting the growth of branches and leaves and flower-bud differentiation at the temperature of between 25 and 30 DEG C in early May when spring twigs sprout basically; and 2, strictly controlling the temperature one month before expected blooming, and treating continuously at the daily mean temperature of less than 24 DEG C or variable diurnal temperature of between 14 and 28 DEG C under the conditions that the humidity is between 70 and 80 percent and that the illumination of daytime is between 3,000 and 10,000 lux until the osmanthus fragrans bloom. According to the regulation and control method, the blooming mechanism of the osmanthus fragrans are found by the studying the flower-bud differentiation, floral organogenesis rule, physiological and biochemical change in the flower-bud differentiation process, the relation between environmental factors and the flower-bud differentiation and the like of different varieties of the osmanthus fragrans; and the temperature is regulated and controlled to promote autumn osmanthus fragrans to bloom a month and a half to two months ahead, so that the flower season of the osmanthus fragrans is regulated, the ornamental period of the osmanthus fragrans is prolonged, and the application value and added value of the potted osmanthus fragran commodities are improved.

The present invention provides a method for expanding and improving functional capacity of human adult-derived progenitor cells in vitro using a closed culture system. The present invention provides a favorable condition for cell therapy to promote tissue repair and organogenesis via vasculogenesis and angiogenesis in clinical settings. The proposed closed bag culture system for culturing hemangioblast comprises of, in one embodiment, a serum-free culture medium containing one or more factors selected from the group consisting of stem cell growth factor, interleukin-6, FMS-like tyrosine kinase 3, thrombopoietin, and vascular endothelial growth factor and a kit for the preparation of the serum-free culture medium and the like.

A populus trichocarpa Torr.Gray leaf adventitious bud induction and plant regeneration method aims to solve the problems that at present, populus trichocarpa Torr.Gray has difficulty in tissue culture organogenesis and low in plant regeneration rate, and the adventitious bud inductivity is low and the period is long since populus trichocarpa Torr.Gray stems are taken as explants. The method provided by the invention comprises the following steps: 1, material collection and treatment; 2, inoculated culture and aseptic seedling expanding propagation: stems which are flushed by tap water are subject to sterile treatment for inoculated culture to propagate aseptic seedlings; 3, adventitious bud differentiation induction; 4, adventitious bud elongation induction: calluses with adventitious buds are cut into blocks and placed in an adventitious bud elongation culture medium for culture; 5, adventitious bud rootage culture; 6, hardening-seedling and transplanting. According to the invention, populus trichocarpa Torr.Gray aseptic seedling leaves are taken as explants, so that the adventitious bud inductivity and the survival rate are up to 100%, and from explant culture to seedling survival, only about 60 days are required. The populus trichocarpa Torr.Gray leaf adventitious bud induction and plant regeneration method is used for plant tissue culture.

The invention discloses a bighead atractylodes rhizome regeneration system for directly differentiating adventitious buds by utilizing hypocotyls and radicles. The bighead atractylodes rhizome regeneration system comprises the following steps: washing and disinfecting bighead atractylodes rhizome seeds; inoculating the disinfected bighead atractylodes rhizome seeds into an MS culture medium; culturing in a dark place until the seeds are germinated; transferring the seeds and continually culturing under light; cutting the radicles and the hypocotyls, cutting into small blocks and taking the small blocks as explants; inoculating the explants into a bud induction culture medium and inducing and culturing; differentiating until green buds grow out; cutting off the green buds and transplanting the green buds into a rooting culture medium for culturing; inducing for rooting; transplanting regenerated plants with roots so as to obtain bighead atractylodes rhizome plants. According to the regeneration system, provided by the invention, the proliferation frequency of bighead atractylodes rhizome is greatly improved, the production cost is saved and the propagation efficiency is improved; technical supports are provided for large-scale production of medical bighead atractylodes rhizome; for the organogenesis which does not subject to calluses, the genetic stability of the plants is higher; quality guarantees are provided for bighead atractylodes rhizome quality improvement, industrial seedling culturing and virus elimination which are realized by utilizing a plant gene engineering technology.

The invention discloses a soybean MADS-box gene and applications thereof in floral organ modification, belonging to the field of biotechnology. GmMADS1, transcription factor in nucleus, is expressed in all the floral organs of four circles and, however, expressed in large quantity in petals and stamens, tobacco expressing excessive GmMADS1 increases the number of sepals, stamens and petals, a transformation from the sepals and the stamens to the petals is formed, as a result, the occurrence of cracking petals and bending stamens etc. causes the stamens to fail to come into contact with pistils, thus normal pollination and normal anther dehiscence cannot be realized and pollen activity is lowered. The above results represent that GmMADS1 plays an important regulation and control role in determining the position and the number of the petals and the development of male organs. New floral organ-variant material, resulted from the GmMADS1 gene, can be used for the breeding of crops and ornamental horticulture plants.

The invention discloses a method of promoting generation of lateral buds of a Heveabrasiliensis by a trans-AtWUS (Arabidopsisthaliana WUSCHEL) gene. Genetic transformation is carried out on a fragile embryonic callus of the Heveabrasiliensis by adopting the AtWUS gene to obtain a transgenetic material to promote generation of the lateral buds of the Heveabrasiliensis and to form lateral branches, so that the function of the AtWUS gene is verified, which shows that the WUS gene has an important effect on generation of the lateral buds and formation of the lateral branches of the Heveabrasiliensis. The lateral buds and lateral branches obtained have actual application potential. By means of the agrobacterium-mediated method, a plant expression vector is established to transfer the AtWUS gene to the callus of the Heveabrasiliensis to obtain the transgenetic material so as to promote somatic embryogenesis or organogenesis of the Heveabrasiliensis and promote the ability of forming a regeneration plant or a budling according to the property that the plant stem cell determinant gene WUS has a key effect on the meristem of a plant and promotes somatic embryogenesis and / or organogenesis. The method serves application and production from a novel path of genetic engineering of the Heveabrasiliensis.

The invention discloses a method for carrying out in-vitro culturing on adventitious buds of juglans mandshurica maxim and inducing plant regeneration, relates to a plant regeneration method of the juglans mandshurica maxim and aims to solve the problems of difficulty, serious browning phenomenon, low inductivity and low rooting rate of existing juglans mandshurica maxim tissue culture organogenesis. The method comprises the following steps: 1, pretreating zygotic embryos of the juglans mandshurica maxim; 2, grafting the pretreated zygotic embryos of the juglans mandshurica maxim to a culture medium for culturing until appearing callus; 3, grafting the callus tissues to a culture medium, and carrying out light and darkness alternation culture until growing adventitious buds; 4, cutting the callus tissues with the adventitious buds into cubes for illumination culture, carrying out elongation of the adventitious buds, and transferring the adventitious buds in a culture medium for carrying out stem drawing of the adventitious buds; 5, transferring the adventitious buds after stem drawing into a culture medium for rooting of the adventitious buds; 6, transplanting the adventitious buds to a greenhouse for culture after hardenining seedlings. According to the method disclosed by the invention, the callu inductivity and the adventitious bud inductivity are up to 100 percent, the stem drawing rate and the rooting rate of the adventitious buds are higher, and the browning rate can be remarkably reduced. The method is applicable to the field of tissue culture of the juglans mandshurica maxim.

The present invention is directed to a method of Acacia mangium regeneration through organogenesis and a method of genetic transformation of Acacia mangium. The method of regeneration comprises inducing callus from different parts of seedlings and vegetatively micropropagated plantlets as explants; adventitious bud induction followed by pinnate leaf and bud elongation and eventually, elongated shoots were induced to root. Based on the regeneration system, a marker gene GUS under cauliflower mosaic virus promoter was introduced to Acacia mangium via Agrobacterium infection. GUS staining in the regenerated plants and Southern blot hybridization prove the incorporation of the foreign gene into the host genome and expression of the foreign gene.

The invention relates to a reproduction method for tissue culture of amorphophallus bulbifer, belonging to the technical field of plant cell engineering. The method comprises the steps of bacterial vaccine culture, embryoid induction, seed ball induction and seed ball maturation culture. By adopting a tissue culture technology, induced development and growth ways of explants are changed; corresponding growth regulation substances are added to guide a growth and development way to be changed into an embryoid generation way from an organ generation way; and a lot of mature amorphophallus bulbifer seed balls are directly obtained through in vitro culture. The reproduction method provided by the invention has the advantages that the sexual reproduction coefficient of the amorphophallus bulbifer is high, the reproduction period is short, the quality is not easy to degenerate, a tissue culture seedling transplanting procedure is simple, and in-vitro tubers are not easily infected with diseases and are easy to germinate; and a practical technology is provided for industrially and rapidly producing the amorphophallus bulbifer seed balls.

The invention discloses a molecular breeding method fast obtaining a large number of transgenosis sedum lineare new species. According to the molecular breeding method, blades, stipes and stems of aseptic seedlings are used as transformation accepters, a plant expression vector of target genes is constructed according to the requirement of the breeding purpose, the target genes are guided into a sedum lineare genome through an agrobacterium tumefaciens mediating method, and a regenerated plant is formed through shoot organogenesis way differentiation; a corresponding antibiotic screening transformant of screening marker genes is added during transformation shoot induction, transformation shoot elongation and rooting, and it is verified that an obtained resistance plant is a transformation plant through Southern hybridization and GUS dyeing verification. According to the method, a large number of target gene transformation sedum lineare new species can be obtained by nine weeks, and the urgent demands for breeding sedum lineare fine strains and innovating and researching genetic resources at present can be met. The molecular breeding method has obvious ecological, economic and social benefit significance in sustainable development of sedum lineare.

The invention provides for a device, system, and methods for using the same with kidney progenitor cells, specifically ureteric bud (UB) cells, metanephric mesenchymal (MM) cells, and the stromal cell (SC) subpopulation of the metanephric mesenchyme, to generate an embryonic kidney organoid that can be implanted into a mammalian subject to create a living, functional kidney.

The present invention provides a commercially viable process for in vitro propagation of patchouli (Pogostemon cablin). The process involves direct organogenesis from meristematic explants employing a simple and cost effective media and shows about 90% survival upon transfer to in vivo conditions.

The invention relates to a method of modulating plant growth or developmental processes in a plant or plant cell, more particularly, organogenesis and / or embryogenesis, through the provision of a plant protein which can act as a transcriptional regulator of the processes. The invention further provides a plant and propagating material thereof which contains, in its genome, a nucleotide sequence encoding a protein of the invention, the protein characterized in that it includes the amino acid sequence PRGRPPGSKNK (SEQ ID NO:2).

The invention provides a broccoli somatic embryo induction plant regeneration method which relates to a somatic embryo induction plant regeneration method and solves the problems of long induced differentiation time, low reproduction rate and high differentiation rate difference among different varieties, which exist in the prior method that broccoli carries out in-vitro regeneration by organogenesis. The broccoli somatic embryo induction plant regeneration method comprises the following steps: 1. sterilizing broccoli seeds; 2. culturing the broccoli seeds to obtain seedlings; 3. cutting explants into small sections, and inducing and culturing to obtain ripe somatic embryos; 4. inoculating the ripe somatic embryos to a 1 / 2MS solid culture medium or a regeneration culture medium to culture; and then, completing broccoli somatic embryo induction plant regeneration. The method has short induced differentiation time, high inductivity and low differentiation rate difference among different varieties.

The invention discloses a method for adventitious bud induction and plant regeneration of rhododendron dauricum, and relates to the field of plant tissue culture, in particular to a method for adventitious bud induction and plant regeneration. The method aims to solve the problems that existing organogenesis tissue culture of rhododendron dauricum is difficult and long in duration. The method includes the steps of 1, material collection and processing; 2, adventitious bud differentiation, induction and proliferation; 3, adventitious bud elongation induction; 4, adventitious bud rooting culture; 5, seedling hardening and transplanting. According to the method, rhododendron dauricum plant water planting tender shoots serve as explants, the adventitious bud induction rate is as high as 100%,the rooting rate of fasciculate seedlings reaches 99%, the transplanting survival rate is up to 100%, and it only takes about 90 days from explant culture to seedling forming; from the second batch, the seedlings are directly cultured from proliferation and differentiation, and it only takes 60 days to obtain the seedlings. The method is applied to the field of plant tissue culture.

The invention discloses a method for directly generating adult seedlings by genetic transformation of septate internodes of mature seed seedlings of a maize inbred line, and belongs to the field of bioengineering. The method comprises the following steps: 1, disinfecting and inoculating mature seeds of the maize inbred line; 2, performing genetic transformation on seedlings obtained by germination of the maize seeds; 3, transferring maize septate internodes subjected to genetic transformation into a co-culture medium; 4, transferring the maize septate internodes from the co-culture medium into an inducing culture medium; 5, performing seedling hardening and indoor transplanting on regenerated seedlings of the maize septate internodes; 6, transplanting the regenerated seedlings of the maize septate internodes into a field. Compared with a way of somatic embryogenesis, the method disclosed by the invention has the advantages that the seedlings are directly generated in an organogenesis way, so that a large quantity of regenerated plants can be obtained quickly within a short period, without being limited by the types of genes and the seasons; materials are convenient to take and are unlikely to pollute; the planting percent is high; a strong foundation is laid for industrial research on maize genetic transformation.

The invention discloses a succeeding preservation method for embryogenic callus and a subculture medium used therein. The succeeding preservation method for embryogenic callus is to subculture embryogenic callus with the subculture medium. The succeeding preservation method for embryogenic callus and the subculture medium provided in the invention has a good culture effect on embryogenic callus of China fir, enable embryogenic competence of callus of China fir to be maintained for a long time, provide great convenience to subsequent research on somatic embryogenesis, organogenesis and the like of China fir and have a great practical application value.

Methods of promoting liver morphogenesis prior to the functioning of blood vessels by culturing liver cells with endothelial cells is provided. Also provided are cell cultures and method of promoting vasculogenesis of liver tissue by contacting liver cells with endothelial cells.