Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Succeeding preservation method for embryogenic callus of China fir and subculture medium used therein

An embryogenic callus and culture medium technology is applied in the field of subculture preservation method of Chinese fir embryogenic callus and its subculture medium, which can solve the problems of effective subculture method medium and the like, and achieve great practical application value. , The effect of cultivation is good, the effect of maintaining embryogenic ability

Inactive Publication Date: 2012-03-14
INST OF BOTANY CHINESE ACAD OF SCI
View PDF3 Cites 11 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The embryogenic callus of Chinese fir is very sensitive to various components and physical and chemical conditions of the medium. So far, there is no effective subculture method and a relatively specific medium at room temperature. Adjust the medium formula for subculture preservation and improve the subculture. Obviously, it is of great significance for the study of somatic embryogenesis

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Succeeding preservation method for embryogenic callus of China fir and subculture medium used therein
  • Succeeding preservation method for embryogenic callus of China fir and subculture medium used therein
  • Succeeding preservation method for embryogenic callus of China fir and subculture medium used therein

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] Embodiment 1, subculture preservation of Chinese fir embryogenic callus

[0061] Method I

[0062] 1. Induction of embryogenic callus of Chinese fir

[0063] The immature Chinese fir cones (purchased from Fuxin Nursery, Qingliu County, Sanming City, Fujian Province) whose embryos had developed to the prezygotic embryo stage were picked and refrigerated at a low temperature of 4°C for 30 days, and then the seeds were taken out from the cones. Soak in 70% alcohol for 1 min, rinse with sterile water for 3 times, then sterilize in 0.1% mercuric chloride solution for 5 min, rinse with sterile water for 3 times. Planting, the explants are transferred in the Erlenmeyer flask (40ml culture medium) that induction medium is housed, and the number of explants in each Erlenmeyer flask is 6, is placed in dark environment, and temperature is 25 ℃, induces After culturing for 30 days, the embryogenic callus of Chinese fir was obtained.

[0064] The above induction medium consists o...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a succeeding preservation method for embryogenic callus and a subculture medium used therein. The succeeding preservation method for embryogenic callus is to subculture embryogenic callus with the subculture medium. The succeeding preservation method for embryogenic callus and the subculture medium provided in the invention has a good culture effect on embryogenic callus of China fir, enable embryogenic competence of callus of China fir to be maintained for a long time, provide great convenience to subsequent research on somatic embryogenesis, organogenesis and the like of China fir and have a great practical application value.

Description

technical field [0001] The invention relates to a subculture preservation method of embryogenic callus and its subculture medium, in particular to a subculture preservation method of fir embryogenic callus and its subculture medium. Background technique [0002] Chinese fir is one of the evergreen coniferous species, and it is a unique fast-growing commercial timber species in my country. Chinese fir grows rapidly, the main stem is straight and round, the material is light and tough, the texture is beautiful, and it is easy to process. At present, the social demand for fine Chinese fir seedlings is extremely urgent, but the traditional method relies on Chinese fir seed gardens to provide seeds, which is easily limited by natural conditions. The problem of unstable sowing quality affects the development of fast-growing and high-yield Chinese fir forests to a certain extent. Therefore, it is necessary to explore the vegetative rapid propagation technology of fine Chinese fir...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 张海燕万里川郭毅
Owner INST OF BOTANY CHINESE ACAD OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com