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Broccoli somatic embryo induction plant regeneration method

A technology of somatic embryos and broccoli, applied in plant regeneration, botanical equipment and methods, horticultural methods, etc., can solve the problems of large difference in differentiation rate, low reproduction rate, and long induction differentiation time

Inactive Publication Date: 2010-01-27
NORTHEAST FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to provide a broccoli somatic embryo in order to solve the problems of long induction differentiation time, low reproduction rate and large difference in differentiation rate among different varieties existing in the in vitro regeneration method of broccoli through organogenesis. Methods of Inducing Plant Regeneration

Method used

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  • Broccoli somatic embryo induction plant regeneration method
  • Broccoli somatic embryo induction plant regeneration method
  • Broccoli somatic embryo induction plant regeneration method

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specific Embodiment approach 1

[0010] Specific embodiment one: the method for inducing plant regeneration of broccoli somatic embryos in the present embodiment is carried out through the following steps: one, first use alcohol with a mass concentration of 65% to 75% to sterilize broccoli seeds for 50 to 70 seconds, and then use mass concentration The hypochlorous acid solution with a concentration of 0.8% to 1.2% is sterilized for 10 to 16 minutes, and then rinsed with sterile water for 3 to 5 times; 2. Inoculate the sterilized broccoli seeds in the germination medium and cultivate them for 6 to 8 days. The broccoli seedlings are obtained when the temperature is 20-25°C; 3. Cut the cotyledons, hypocotyls or roots of the broccoli seedlings into 0.5-10 mm segments with a knife and inoculate them into solid induction medium or liquid induction medium Cultivate at a temperature of 20-25°C, and culture for 7-10 days without light to obtain spherical embryos, then separate the spherical embryos from the mother and...

specific Embodiment approach 2

[0014] Specific embodiment two: what this embodiment is different from specific embodiment one is that in step one, be that the alcohol of 70% is sterilized broccoli seed 60s with mass concentration earlier, then be the hypochlorous acid solution disinfection 15min of 1% with mass concentration, Then rinse 3 times with sterile water. Other steps and parameters are the same as those in Embodiment 1.

specific Embodiment approach 3

[0015] Embodiment 3: The difference between this embodiment and Embodiment 1 or 2 is that in step 2, the sterilized broccoli seeds are inoculated in the germination medium and cultivated for 7 days at 23°C. Other steps and parameters are the same as those in Embodiment 1 or Embodiment 2.

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Abstract

The invention provides a broccoli somatic embryo induction plant regeneration method which relates to a somatic embryo induction plant regeneration method and solves the problems of long induced differentiation time, low reproduction rate and high differentiation rate difference among different varieties, which exist in the prior method that broccoli carries out in-vitro regeneration by organogenesis. The broccoli somatic embryo induction plant regeneration method comprises the following steps: 1. sterilizing broccoli seeds; 2. culturing the broccoli seeds to obtain seedlings; 3. cutting explants into small sections, and inducing and culturing to obtain ripe somatic embryos; 4. inoculating the ripe somatic embryos to a 1 / 2MS solid culture medium or a regeneration culture medium to culture; and then, completing broccoli somatic embryo induction plant regeneration. The method has short induced differentiation time, high inductivity and low differentiation rate difference among different varieties.

Description

technical field [0001] The invention relates to a method for inducing plant regeneration with somatic embryos. Background technique [0002] Broccoli (Brassica oleracea L.var.italica Planch), also known as broccoli, green cauliflower, stem broccoli, woody cauliflower, Italian kale, etc., is a one-year or biennial herb with high nutritional value, rich in vitamin A and Vitamin C, with respectable amounts of B vitamins 1 , B 2 , B 5 , calcium and iron and other substances, and contains anti-cancer substances, favored by consumers. The cultivation history of broccoli is relatively short, but it has developed rapidly. It has become one of the important high-quality vegetable varieties for export and foreign exchange earning in some areas of our country. At the same time, the first-generation hybrid varieties bred in the United States, Japan and other countries sell well in domestic and foreign markets, and a number of varieties have also been bred in my country. [0003] Th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 李成浩杨静莉刘立琨李俊秀周晨光陈爱华
Owner NORTHEAST FORESTRY UNIVERSITY
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