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Conjoined grafting method for plant meristem

A meristematic tissue and plant technology, applied in the field of plant genetic grafting research and application, can solve the problems of uncontrollable randomness of transgenic sites and effects, high cost of cloning genes, and restrictions on large-scale utilization of in vitro transgenic technology. The chance of successful grafting, the effect of low cost

Inactive Publication Date: 2013-06-19
YANGTZE UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Although modern in vitro transgenic technology can theoretically break the boundaries of any species, and even carry out the transformation and exchange of some genes between different domains of prokaryotes and eukaryotes, the cost of cloning genes in vitro is very high. When the gene is transferred into the recipient again, there are limitations such as uncontrollability and randomness of the transgenic site and effect. At the same time, people still have certain concerns about the safety and gene escape of in vitro artificial transgenic products, resulting in high cost of transgenic technology. , the effect is difficult to determine
These difficulties of control and the risk of gene escape limit the large-scale utilization of ex vivo transgenic technology

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1-Grafting between different gates:

[0033] Cycad: Cycadophyta, Cycas class, Cycas order, Cycadaceae, Cycads genus (cycas);

[0034] Plantain (M.basjoo): Angiosperm (Magnoliophyta), Monocotyledons, Zingiberaceae, Musaceae, Musa (Musa).

[0035]Use the secondary roots of cycads as scion-like, and the secondary roots of plantain as stock-like to graft to obtain conjoined grafted plants. Then the growth of the plants of the conjoined grafted cycads and plantains changes, and the cycads begin to grow from 100cm to 150cm in height. It became shorter again, only 15cm left at present, and the plantain plant of the original type rootstock was much shorter than the ungrafted plant (about 150cm) at that time, only about 100cm at present.

[0036] Sensitive to herbicides. Sensitive to inorganic nitrogen fertilizers and white potassium fertilizers, it attracts parrots to feed on them. To prevent and control bird damage, use organic fertilizers or farmyard manures or ...

Embodiment 2

[0038] Grafting between different classes of embodiment 2-:

example 21

[0040] Rubber tree (H.brasiliensis): Magnoliopsida, Euphorbia, Euphorbiaceae, Hevea;

[0041] Plantain (M.basjoo): Monocotyledons (Liliopsida), Zingiberaceae, Musaceae, Musa (Musa).

[0042] A. Use rubber trees as scion-like scions, and virgin forest high-altitude wild plantains as rootstocks, and carry out secondary root grafting to produce budding plants with high resistance to powdery mildew and anthracnose, which improves the disease resistance and yield of rubber trees.

[0043] B. Using rubber trees as scion-like scions and virgin forest high-altitude wild plantains as rootstocks, intermediary meristem grafting is carried out to produce budding plants with high resistance to powdery mildew and anthracnose, which improves the disease resistance and yield of rubber trees.

[0044] Plantain rubber trees are sensitive to herbicides. Sensitive to inorganic nitrogen fertilizers and white potassium fertilizers, it attracts parrots to feed on them. To prevent and control bird ...

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Abstract

The invention relates to a conjoined grafting method for plant meristem. The conjoined grafting method for the plant meristem is characterized in that the conjoined grafting method through meristem between different phylum, klasse, order, family, genus and species of plantae is provided, and the conjoined grafting method comprises an apcial meristem, a lateral meristem and an intercalary meristem. The meristem possesses massive reversible gene regulatory networks and spatial and temporal expression opportunities due to the fact that the meristem has high cell division ability and is a key tissue of organogenesis and morphogenesis and a starting point of organ and tissue differentiation. Therefore, conducting moderate manual injury intervention and conjoined grafting on the meristem can greatly increase opportunity of successful grafting, and enlarges boundary capable of being conducted grafting between different phylum, klasse, order, family, genus and species of the plantae. Due to the fact that scion and rootstock whole plant are not separated, bud mutation and fructification are promoted to obtain grafting progeny seeds, exchanging and changing of part genetic materials of grafting progeny can be achieved, and consequently, low coat and safe material exchanging of extra distant species of the plantae can be achieved, variation is induced, and then a new germplasm of a scion plant is created.

Description

Technical field: [0001] The invention relates to a method for conjoined grafting of plant meristems, which belongs to the technical field of research and application of plant genetic grafting. Background technique: [0002] Species evolution is the source of biodiversity on which human beings depend, and domain, kingdom, phylum, class, order, family, genus, and species are the basic units of biological classification. During tens of thousands of years of natural evolution of species, the natural world has formed the basic characteristics that there is generally no biological isolation within a species, and it is easy to carry out natural and artificial hybridization. Sex is stable inheritance. But at the same time, there are also complex interactions such as various special symbiosis, antagonism, mutualism, inhibition, toxicity, etc. among different domains, kingdoms, phyla, classes, orders, families, genus, and species to a certain extent. A part of variation, heredity an...

Claims

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Application Information

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IPC IPC(8): A01G1/06
Inventor 李晓方李志新曹旭朴曹文义曹旭琦
Owner YANGTZE UNIVERSITY
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