Conjoined grafting method for plant meristem

Abstract

The invention relates to a conjoined grafting method for plant meristem. The conjoined grafting method for the plant meristem is characterized in that the conjoined grafting method through meristem between different phylum, klasse, order, family, genus and species of plantae is provided, and the conjoined grafting method comprises an apcial meristem, a lateral meristem and an intercalary meristem. The meristem possesses massive reversible gene regulatory networks and spatial and temporal expression opportunities due to the fact that the meristem has high cell division ability and is a key tissue of organogenesis and morphogenesis and a starting point of organ and tissue differentiation. Therefore, conducting moderate manual injury intervention and conjoined grafting on the meristem can greatly increase opportunity of successful grafting, and enlarges boundary capable of being conducted grafting between different phylum, klasse, order, family, genus and species of the plantae. Due to the fact that scion and rootstock whole plant are not separated, bud mutation and fructification are promoted to obtain grafting progeny seeds, exchanging and changing of part genetic materials of grafting progeny can be achieved, and consequently, low coat and safe material exchanging of extra distant species of the plantae can be achieved, variation is induced, and then a new germplasm of a scion plant is created.

Description

Technical field:

[0001] The invention relates to a method for conjoined grafting of plant meristems, which belongs to the technical field of research and application of plant genetic grafting. Background technique:

[0002] Species evolution is the source of biodiversity on which human beings depend, and domain, kingdom, phylum, class, order, family, genus, and species are the basic units of biological classification. During tens of thousands of years of natural evolution of species, the natural world has formed the basic characteristics that there is generally no biological isolation within a species, and it is easy to carry out natural and artificial hybridization. Sex is stable inheritance. But at the same time, there are also complex interactions such as various special symbiosis, antagonism, mutualism, inhibition, toxicity, etc. among different domains, kingdoms, phyla, classes, orders, families, genus, and species to a certain extent. A part of variation, heredity an...