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Reproduction method for tissue culture of amorphophallus bulbifer

A technology of tissue culture and konjac, which is applied in the field of plant cell engineering, can solve the problems of rapid propagation technology research of unjin konjac varieties, long bulb cycle of bulbil konjac bulbs, and low bulb reproduction coefficient, so as to achieve quality that is not easily degraded, vitality and Strong stress resistance and large single grain diameter

Inactive Publication Date: 2017-02-22
KUNMING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, there are still some shortcomings in the cultivation of golden konjac, such as large amount of seeds, low bulb propagation coefficient, easy propagation of konjac diseases by cutting into pieces, small amount of seeds, and low variation. With the development of konjac industry, it is urgently needed A large number of high-quality konjac seedlings
Although under laboratory conditions, there has been a breeding method for the propagation of Amorphophallus bulbils (referring to the red Amorphophallus variety) through tissue culture, but there is no related research on the tissue rapid propagation technology of the golden Amorphophallus variety
In addition, the above-mentioned method requires multi-step procedures such as rooting, seedling hardening, and transplanting for the rapid propagation of Amorphophallus konjac (red Amorphophallus), and there are problems such as long cycle for obtaining Amorphophallus bulbils, slow growth, and intolerance to storage and transportation.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A kind of propagation method related to the tissue culture of bulbil Amorphophallus konjac, comprising the steps of aseptic seedling culture, embryoid body induction, seed bulb induction, and seed bulb maturation culture:

[0023] 1) Sterile seedling cultivation: After the young leaves, petioles and clustered buds of the bulbil class konjac plants are routinely sterilized, the leaves are cut into 0.5-1.0cm2, the petioles are cut into 0.8-1cm long, and inoculated into conventionally sterilized seedlings On the culture medium, culture under 2000lux light, 11h / d, culture temperature 28±1℃, to obtain virus-free and sterile seedlings; the composition and ratio of the seedling medium are: MS basic medium + 0.2mg / L NAA + 0.5 mg / L 6-BA+activated carbon 0-3.0g / L, sucrose 30g / L, agar 6-8g / L, adjust the pH to 5.8-6.2;

[0024] 2) embryoid body induction: the detoxified aseptic seedlings obtained in step 1), get the leaves and cut them into 0.5-1.0cm 2, cut the petioles into 0.8-1....

Embodiment 2

[0034] A kind of propagation method related to the tissue culture of bulbil Amorphophallus konjac, comprising the steps of aseptic seedling culture, embryoid body induction, seed bulb induction, and seed bulb maturation culture:

[0035] 1) Sterile seedling cultivation: After the young leaves, petioles and clustered buds of the bulbil class konjac plants are routinely sterilized, the leaves are cut into 0.5-1.0cm2, the petioles are cut into 0.8-1cm long, and inoculated into conventionally sterilized seedlings On the culture medium, culture under 2000lux light, 11h / d, culture temperature 28±1℃, to obtain virus-free and sterile seedlings; the composition and ratio of the seedling medium are: MS basic medium + 0.2mg / L NAA + 0.5 mg / L 6-BA+activated carbon 0-3.0g / L, sucrose 30g / L, agar 6-8g / L, adjust the pH to 5.8-6.2;

[0036] 2) embryoid body induction: the detoxified aseptic seedlings obtained in step 1), get the leaves and cut them into 0.5-1.0cm 2, cut the petioles into 0.8-1....

Embodiment 3

[0047] A kind of propagation method related to the tissue culture of bulbil Amorphophallus konjac, comprising the steps of aseptic seedling culture, embryoid body induction, seed bulb induction, and seed bulb maturation culture:

[0048] 1) Sterile seedling cultivation: After the young leaves, petioles and clustered buds of the bulbil class konjac plants are routinely sterilized, the leaves are cut into 0.5-1.0cm2, the petioles are cut into 0.8-1cm long, and inoculated into conventionally sterilized seedlings On the culture medium, culture under 2000lux light, 11h / d, culture temperature 28±1℃, to obtain virus-free and sterile seedlings; the composition and ratio of the seedling medium are: MS basic medium + 0.2mg / L NAA + 0.5 mg / L 6-BA+activated carbon 0-3.0g / L, sucrose 30g / L, agar 6-8g / L, adjust the pH to 5.8-6.2;

[0049] 2) embryoid body induction: the detoxified aseptic seedlings obtained in step 1), get the leaves and cut them into 0.5-1.0cm 2, cut the petioles into 0.8-1....

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Abstract

The invention relates to a reproduction method for tissue culture of amorphophallus bulbifer, belonging to the technical field of plant cell engineering. The method comprises the steps of bacterial vaccine culture, embryoid induction, seed ball induction and seed ball maturation culture. By adopting a tissue culture technology, induced development and growth ways of explants are changed; corresponding growth regulation substances are added to guide a growth and development way to be changed into an embryoid generation way from an organ generation way; and a lot of mature amorphophallus bulbifer seed balls are directly obtained through in vitro culture. The reproduction method provided by the invention has the advantages that the sexual reproduction coefficient of the amorphophallus bulbifer is high, the reproduction period is short, the quality is not easy to degenerate, a tissue culture seedling transplanting procedure is simple, and in-vitro tubers are not easily infected with diseases and are easy to germinate; and a practical technology is provided for industrially and rapidly producing the amorphophallus bulbifer seed balls.

Description

technical field [0001] The invention belongs to the technical field of plant cell engineering, and relates to a plant propagation method, in particular to a method for rapid propagation of bulbil konjac tissue culture. Background technique [0002] Konjac is a perennial herbaceous plant of the genus Amorphophallus of the Araceae family. It is the only plant population found so far that can synthesize a large amount of glucomannan (konjac glucomannan, KGM). It is mainly distributed in tropical and subtropical regions of Asia and Africa. Only a few Asian countries have cultivation, and China and Japan are more common. It is an important characteristic economic crop in Southwest my country. It has been cultivated and eaten for more than 2,000 years. Its main component, glucomannan, is an excellent pure natural dietary fiber with high economic value. It has been used in food, health care Products, medicine, chemical industry, petroleum, textile and other fields have been widely ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 余磊赵建荣刘佳妮徐胜光陈泽斌苏源王定康郭丽红
Owner KUNMING UNIV
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