Pinellia tuber artificial seed stem production method

A production method, pinellia technology, applied in the field of plant tissue culture, can solve the problems of pinellia wild resources depletion, insufficient supply of provenance, slow growth, etc., to alleviate the shortage of provenance in the market, strong vitality and stress resistance , to overcome the effect of low reproduction coefficient

Inactive Publication Date: 2009-07-29
九信(武汉)中药研究院有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the low sexual reproduction coefficient of pinellia, it mainly relies on tubers for asexual reproduction, and some soil-borne diseases such as viral diseases and root rot are prone to occur, resulting in low yield and quality degradation. Due to its low natural reproduction coefficient and low yield High, large-scale planting is affected by the insufficient supply of provenance, coupled with the indiscriminate harvesting of wild resources, the wild resources of pinellia are on the verge of depletion
[0004] At present, under laboratory conditions, there have been mature techniques for rapidly propagating Pinellia seedlings through tissue culture and transplanting them into field to survive (Zhang Zhenyuan, Research on Tissue Culture Techniques of Different Lines of Jingpinia, 2008 Master Thesis; Hao Huijun et al., Pinellia Tissue culture seedling hardening technology research, Anhui Agricultural Sciences 2008, 36 (5): 1918-1919 and 2018), but this method needs to carry out multi-step procedures such as rooting, hardening, transplanting, etc., and the cycle of obtaining Pinellia tuber is long, grow slowly

Method used

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  • Pinellia tuber artificial seed stem production method
  • Pinellia tuber artificial seed stem production method
  • Pinellia tuber artificial seed stem production method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A kind of artificial stem production method of pinellia, the steps are as follows:

[0027] (1) Aseptic seedling cultivation: After sterilizing the young leaves or petioles of wild pinellia plants in Guidong County, Hunan, cut the leaves into 0.5-1.0cm 2 , the petiole is cut into 0.8-1cm long, and inoculated on the aseptic seedling medium. The components and ratio of the aseptic seedling medium are: MS basic medium + 0.2mg / L naphthaleneacetic acid + 0.5mg / L 6-benzyl Aminopurine + activated carbon 1.0-3.0g / L, sucrose 30g / L, agar 6-8g / L, adjust the pH to 5.8-6.0, the medium is sterilized by conventional methods, that is, steam sterilization at 121-126°C for 30min , standby, the same below. After inoculation, the culture is cultivated under 2000lux light, 12h / d, and the culture temperature is 27±1℃ to obtain virus-free and sterile vaccines;

[0028] (2) Embryoid body induction: the detoxified sterile seedlings obtained in step (1), get the leaves and cut them into 0.5-1....

Embodiment 2

[0032] According to the basic steps described in embodiment 1, wherein:

[0033] The stem maturation medium component described in step (4) and its proportioning ratio are: MS basic medium+0.2mg / L naphthaleneacetic acid+0.5mg / L6-benzylaminopurine+30g / L polyethylene glycol, Others are the same.

Embodiment 3

[0035] According to the basic steps described in embodiment 1, wherein:

[0036] The stem maturation medium component described in step (4) and its proportioning ratio are: MS basic medium+0.2mg / L naphthaleneacetic acid+0.5mg / L6-benzylaminopurine+50g / L polyethylene glycol, Other compositions and conditions are with embodiment 1.

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Abstract

The invention belongs to the technical field of plant tissue culture, in particular to a production method for a pinellia tuber artificial seed stem. The method comprises the following steps: the selection and disinfection of a pinellia tuber explant, the induction and propagation of an embryoid, and the induction of the formation, growth and maturation of the artificial seed stem. The corresponding procedure from the wild pinellia tuber plant to the artificial seed stem production, and the key technology of exclusive culture mediums needed in different phases can be used to culture and produce the mature pinellia tuber artificial seed stem at the large scale; and the sprouting rate can reach 90 percent. The method overcomes the defects of low pinellia tuber sexual propagation coefficient, long propagation cycle, easily degenerated quality, troublesome tissue culture seedling transplanting procedure, immature and hardly sprouting in-vitro tuber, and the like under the natural conditions, provides the practical technology for the rapid industrialized production of the pinellia tuber artificial seed stem, and can alleviate the short supply status quo of the seed market.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture. In particular, it relates to a rapid propagation method in vitro for artificially mature seed stems of Pinellia pinellia. Background technique [0002] Pinellia ternate. (Thunb.) Breit., belongs to Araceae plant, herb, plant height 15-30cm. Seedlings are usually single leaves, egg-shaped and heart-shaped, compound leaves with 3 leaflets after 2-3 years, and the leaves are entire; the petiole is 10-25cm long, and there are bulbils at the base. The flowers are unisexual, the inflorescence stalk is longer than the petiole, the spathe is green, and the lower part is thin tube-shaped; the female flower is born at the base of the catkins, the male flower is born at the upper end, and the female inflorescence and the spadix are conjoined to reach the septum in the lower part of the spadix, flowering on one side, Male inflorescences above septum, apically with elongated, linear appendages,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 王沫叶双凤
Owner 九信(武汉)中药研究院有限公司
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