Pinellia tuber artificial seed stem production method
A production method, pinellia technology, applied in the field of plant tissue culture, can solve the problems of pinellia wild resources depletion, insufficient supply of provenance, slow growth, etc., to alleviate the shortage of provenance in the market, strong vitality and stress resistance , to overcome the effect of low reproduction coefficient
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Embodiment 1
[0026] A kind of artificial stem production method of pinellia, the steps are as follows:
[0027] (1) Aseptic seedling cultivation: After sterilizing the young leaves or petioles of wild pinellia plants in Guidong County, Hunan, cut the leaves into 0.5-1.0cm 2 , the petiole is cut into 0.8-1cm long, and inoculated on the aseptic seedling medium. The components and ratio of the aseptic seedling medium are: MS basic medium + 0.2mg / L naphthaleneacetic acid + 0.5mg / L 6-benzyl Aminopurine + activated carbon 1.0-3.0g / L, sucrose 30g / L, agar 6-8g / L, adjust the pH to 5.8-6.0, the medium is sterilized by conventional methods, that is, steam sterilization at 121-126°C for 30min , standby, the same below. After inoculation, the culture is cultivated under 2000lux light, 12h / d, and the culture temperature is 27±1℃ to obtain virus-free and sterile vaccines;
[0028] (2) Embryoid body induction: the detoxified sterile seedlings obtained in step (1), get the leaves and cut them into 0.5-1....
Embodiment 2
[0032] According to the basic steps described in embodiment 1, wherein:
[0033] The stem maturation medium component described in step (4) and its proportioning ratio are: MS basic medium+0.2mg / L naphthaleneacetic acid+0.5mg / L6-benzylaminopurine+30g / L polyethylene glycol, Others are the same.
Embodiment 3
[0035] According to the basic steps described in embodiment 1, wherein:
[0036] The stem maturation medium component described in step (4) and its proportioning ratio are: MS basic medium+0.2mg / L naphthaleneacetic acid+0.5mg / L6-benzylaminopurine+50g / L polyethylene glycol, Other compositions and conditions are with embodiment 1.
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