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Succeeding preservation method for embryogenic callus of China fir and subculture medium used therein

A technology of embryogenic callus and culture medium, applied in the subculture preservation method of Chinese fir embryogenic callus and its subculture medium, can solve the problems of effective subculture method culture medium, etc., and achieve great practical application value , maintain the ability of embryonicity, and the effect of cultivating is good

Inactive Publication Date: 2013-10-09
INST OF BOTANY CHINESE ACAD OF SCI
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  • Application Information

AI Technical Summary

Problems solved by technology

The embryogenic callus of Chinese fir is very sensitive to various components and physical and chemical conditions of the medium. So far, there is no effective subculture method and a relatively specific medium at room temperature. Adjust the medium formula for subculture preservation and improve the subculture. Obviously, it is of great significance for the study of somatic embryogenesis

Method used

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  • Succeeding preservation method for embryogenic callus of China fir and subculture medium used therein
  • Succeeding preservation method for embryogenic callus of China fir and subculture medium used therein
  • Succeeding preservation method for embryogenic callus of China fir and subculture medium used therein

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Embodiment 1

[0060] Embodiment 1, subculture preservation of Chinese fir embryogenic callus

[0061] Method I

[0062] 1. Induction of embryogenic callus of Chinese fir

[0063] The immature fir cones (purchased from Fuxin Nursery, Qingliu County, Sanming City, Fujian Province) whose embryos had developed to the prezygotic embryo stage were picked and refrigerated at 4°C for 30 days, then the seeds were taken out from the cones, and the seeds were Soak in 70% alcohol for 1 min, rinse with sterile water for 3 times, then sterilize in 0.1% mercuric chloride solution for 5 min, rinse with sterile water for 3 times. Planting, the explants are transferred in the Erlenmeyer flask (40ml culture medium) that induction medium is housed, and the number of explants in each Erlenmeyer flask is 6, is placed in dark environment, and temperature is 25 ℃, induces After culturing for 30 days, the embryogenic callus of Chinese fir was obtained.

[0064] The above induction medium consists of the followin...

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Abstract

The invention discloses a succeeding preservation method for embryogenic callus and a subculture medium used therein. The succeeding preservation method for embryogenic callus is to subculture embryogenic callus with the subculture medium. The succeeding preservation method for embryogenic callus and the subculture medium provided in the invention has a good culture effect on embryogenic callus of China fir, enable embryogenic competence of callus of China fir to be maintained for a long time, provide great convenience to subsequent research on somatic embryogenesis, organogenesis and the like of China fir and have a great practical application value.

Description

technical field [0001] The invention relates to a subculture preservation method of embryogenic callus and its subculture medium, in particular to a subculture preservation method of fir embryogenic callus and its subculture medium. Background technique [0002] Chinese fir is one of the evergreen coniferous species, and it is a unique fast-growing commercial timber species in my country. Chinese fir grows rapidly, the main stem is straight and round, the material is light and tough, the texture is beautiful, and it is easy to process. At present, the social demand for fine Chinese fir seedlings is extremely urgent, but the traditional method relies on Chinese fir seed gardens to provide seeds, which is easily limited by natural conditions. The problem of unstable sowing quality affects the development of fast-growing and high-yield Chinese fir forests to a certain extent. Therefore, it is necessary to explore the vegetative rapid propagation technology of fine Chinese fir...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/04A01H4/00
Inventor 张海燕万里川郭毅
Owner INST OF BOTANY CHINESE ACAD OF SCI
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