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Method for adventitious bud induction and plant regeneration of rhododendron dauricum

A technology of bud induction and planting, which is applied in the field of plant tissue culture, can solve the problems of difficulty in cultivating organs and long time, and achieve the effects of broad application prospects, consistency, and simple operation steps

Active Publication Date: 2019-04-16
黑龙江岭山农业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The present invention aims to solve the problem of difficult and long-time-consuming tissue culture organs of the existing Rhododendron xing'an, and provides a method for inducing adventitious buds and plant regeneration of Rhododendron xing'an

Method used

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  • Method for adventitious bud induction and plant regeneration of rhododendron dauricum
  • Method for adventitious bud induction and plant regeneration of rhododendron dauricum
  • Method for adventitious bud induction and plant regeneration of rhododendron dauricum

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specific Embodiment approach 1

[0033] Specific embodiment one: the method for induction of adventitious buds and plant regeneration of rhododendron xing'an in this embodiment comprises the following steps:

[0034] 1. Material collection and processing:

[0035] Select wild Xing’an Rhododendron plants that are robust and free from diseases and insect pests, cut off 40-50cm long branches, and perform hydroponics treatment. Cut off when the tender stems grow to 3-5cm, rinse with tap water for 1-2 hours, and place them on a sterile operating table. Disinfect inside;

[0036] 2. Adventitious bud differentiation induction and proliferation:

[0037] Inoculate the sterilized materials in Step 1 into adventitious bud induction medium WPM1 for cultivation, the cultivation conditions are: temperature 23-25°C, daily light 12-16h, light intensity 30-40μmol·m -2 ·s -1 Adventitious buds can be induced by culturing for 15-20 days; then continue to be placed in the culture medium WPM1 for the proliferation of adventiti...

specific Embodiment approach 2

[0044] Specific embodiment two: the difference between this embodiment and specific embodiment one is that the specific method of disinfection treatment described in step one is: the tender stems washed with tap water are washed once with sterile water, and then the stems are put into Disinfect in 70% alcohol for 10 sec, take out the stem segment and wash it 3 times with sterile water, then disinfect it with 3% sodium hypochlorite for 40 sec, wash it again with sterile water for 3 times, and finally use sterile filter paper Blot the moisture on the surface of the tender stem, and use sterile scissors to cut off the part of the tender stem morphology that has been oxidized by the disinfectant solution, and it is completed. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0045] Specific embodiment three: the difference between this embodiment and specific embodiment one or two is: the adventitious bud induction medium WPM1 described in the step 2 is made of 400mg / L NH 4 NO 3 , 370mg / L MgSO 4 ·7H 2 O, 990mg / L K 2 SO 4 、170mg / LKH 2 PO 4 , 96mg / L CaCl 2 2H 2 O, 27.8mg / L FeSO 4 ·7H 2 O, 37.3mg / L Na 2 EDTA, 8.6mg / L ZnSO 4 ·7H 2 O, 0.25mg / L CuSO 4 ·5H 2 O, 22.4mg / L MnSO 4 4H 2 O, 6.2 mg / L H 3 BO 3 , 0.25mg / L NaMoO 2 2H 2 O, 1.0mg / L thiamine hydrochloride, 0.5mg / L pyridoxine hydrochloride, 0.5mg / L niacin, 2.0mg / L glycine, 100mg / L inositol, 25g / L sucrose, 6.5g / L agar, Made from 0.01-1.0mg / L indolebutyric acid, 0.01-1.0mg / L thiadizuron and distilled water, with a pH value of 5.5. Others are the same as in the first or second embodiment.

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Abstract

The invention discloses a method for adventitious bud induction and plant regeneration of rhododendron dauricum, and relates to the field of plant tissue culture, in particular to a method for adventitious bud induction and plant regeneration. The method aims to solve the problems that existing organogenesis tissue culture of rhododendron dauricum is difficult and long in duration. The method includes the steps of 1, material collection and processing; 2, adventitious bud differentiation, induction and proliferation; 3, adventitious bud elongation induction; 4, adventitious bud rooting culture; 5, seedling hardening and transplanting. According to the method, rhododendron dauricum plant water planting tender shoots serve as explants, the adventitious bud induction rate is as high as 100%,the rooting rate of fasciculate seedlings reaches 99%, the transplanting survival rate is up to 100%, and it only takes about 90 days from explant culture to seedling forming; from the second batch, the seedlings are directly cultured from proliferation and differentiation, and it only takes 60 days to obtain the seedlings. The method is applied to the field of plant tissue culture.

Description

technical field [0001] The invention relates to the field of plant tissue culture, in particular to a method for inducing adventitious buds and plant regeneration. Background technique [0002] Rhododendron dauricum L. belongs to Rhododendron dauricum L., Rhododendron dauricum L. Wild Xing'an Rhododendron, also known as Dazixiang, Jindalai, and Yingshanhong, likes light, tolerates half shade, likes cold and humid climates, and is suitable for growing in slightly acidic soil. . In China, it is mainly distributed in Inner Mongolia, Jilin and Heilongjiang's Greater and Lesser Xing'an Mountains, Wanda Mountain, Zhangguangcai Mountain and Laoye Mountain. It is also distributed in northern Korea, Mongolia, Japan, Eastern Siberia and the Far East of the Soviet Union. Xing'an Rhododendron is a rare wild shrub flower. Because wild Xing'an Rhododendron has the characteristics of long flowering period, bright color, and easy survival, Xing'an Rhododendron has been excavated in large...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 李淑娟张含国董实伟董昊白晓明杨宇宁史瀛
Owner 黑龙江岭山农业科技有限公司
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