Molecular breeding method fast obtaining large number of transgenosis sedum lineare new species

Abstract

The invention discloses a molecular breeding method fast obtaining a large number of transgenosis sedum lineare new species. According to the molecular breeding method, blades, stipes and stems of aseptic seedlings are used as transformation accepters, a plant expression vector of target genes is constructed according to the requirement of the breeding purpose, the target genes are guided into a sedum lineare genome through an agrobacterium tumefaciens mediating method, and a regenerated plant is formed through shoot organogenesis way differentiation; a corresponding antibiotic screening transformant of screening marker genes is added during transformation shoot induction, transformation shoot elongation and rooting, and it is verified that an obtained resistance plant is a transformation plant through Southern hybridization and GUS dyeing verification. According to the method, a large number of target gene transformation sedum lineare new species can be obtained by nine weeks, and the urgent demands for breeding sedum lineare fine strains and innovating and researching genetic resources at present can be met. The molecular breeding method has obvious ecological, economic and social benefit significance in sustainable development of sedum lineare.

Description

technical field [0001] The invention belongs to the technical field of plant genetic engineering, and in particular relates to a molecular breeding method for quickly obtaining a large number of new varieties of transgenic S. argentina. Background technique [0002] Roof greening has become an important development research direction for building three-dimensional space greening in today's society because of its functions of increasing green space, heat insulation, cooling and dust removal, air purification, landscaping, energy saving and water saving. An important element of the roof greening project is to discover and cultivate greening materials with excellent characteristics. The current plant breeding methods include traditional hybridization, introduction and domestication, budding, physical and chemical mutagenesis, and molecular breeding based on transgenic technology. Various breeding methods have their advantages and disadvantages: traditional hybrid breeding is a...

AI Technical Summary

Problems solved by technology

Various breeding methods have their advantages and disadvantages: traditional hybrid breeding is a commonly used method and has achieved brilliant achievements, but the conventional excellent hybrid combination breeding technology has a long cycle, low efficiency, and poor predictability of offspring phenotypes; Although the physical and chemical mutation breeding method has also played a very important role in cultivating high-quality varieties of plants, because this method is a one-time mutagenesis, the treatment is cumbersome and the breeding cycle is long, the frequency of beneficial mutations is low, and the physical and chemical mutations are general. Only for a certain stage of material growth, and the mutagenic effects of plant materials are different in different growth stages, which also leads to a relatively single mutant character produced by physical and chemical mutagenesis, and the number of new varieties cultivated has gradually decreased in recent years, and this method It is also impossible to accurately operate and select a certain gene, and the performance of offspring is also poorly predictable; and genetic engineering based on molecular genetics and technical methods of molecular biology and microbiology is to Genes from different sources, according to the pre-designed blueprint, construct recombinant genes in vitro, and then introduce them into cells to change the original genetic characteristics of the organism. After field experiments and field selection, new transgenic varieties or germplasm resources with ideal traits are bred

At present, there are no patent documents and other documents about the technology of molecular breeding and regeneration plant cultivation of S.

Images