Method of cutting propagation of peony immature stem

Abstract

The invention belongs to the field of plant tissue cultivation, and particularly relates to a method of cutting propagation of a peony immature stem. The method includes the steps of material selection, cutting cultivation, indoor management, oversummer maintenance, overwinter management and strong seedling cultivation. By using low-frequency ultrasonic waves to conduct processing, rooting can be promoted, plant cell division can be remarkably accelerated and induced, the cell growth is stimulated, protein synthesis of protoplast can be accelerated, and the adventitious bud reproducibility can be improved. Due to the fact that intermittent irradiation is adopted, no drastic cavitation effect can be produced, and partial damage to cells caused by the ultrasonic waves can be avoided. Due to the fact that cultivation and rooting are conducted in the dark cultivation process, growth inhibitor is greatly reduced, and rooting is facilitated. The complete and specific method including the steps of oversummer maintenance, auxiliary bud inducing, overwinter management and strong seedling cultication is provided, and therefore application of the peony rapid cutting propagation technology in actual production is promoted, and the method has significance in large-scale production of peony seedlings.

Description

technical field [0001] The invention belongs to the field of plant tissue culture, and in particular relates to a method for cutting and propagating tender stems of peony. Background technique [0002] Peony (Paeonia lactiflora Pall) is a perennial herbaceous plant of Paeoniaceae Paeoniaceae. It has the reputation of "flower appearance" since ancient times. Its flowers have the beauty of color, fragrance and rhyme. As a traditional famous flower in China, peony has broad application prospects in the production of fresh-cut flowers in addition to landscaping and medicinal uses. However, the production of peony seedlings is relatively lagging behind and cannot meet market demand. Most ornamental peony varieties lose or partly lose their fruitiness due to the high rate of pistil and stamen petalization, and the sowing and reproduction cannot maintain the female parent traits, so the traditional branching method is often used for propagation. A three-year-old peony seedling is...

AI Technical Summary

Problems solved by technology

[0004] At present, there are still problems such as low proliferation rate of adventitious buds and difficulty in transplanting and surviving tissue cultured seedlings, which cannot meet the requirements of practical application level.

In addition, there are still large gaps in the research on oversummer maintenance of rooted seedlings, induction of axillary buds, overwintering management, and cultivation of strong seedlings.