SNP (single nucleotide polymorphism) site related to characters of plant root system and application thereof

A plant root system and site technology, applied in the biological field, can solve the problems of few tightly linked markers

Active Publication Date: 2014-07-30
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most genes for root traits are associated with genes related to fertilizer and water use, agronomic traits, and yield traits (Bai et al., 2013), but few closely linked markers have been reported

Method used

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  • SNP (single nucleotide polymorphism) site related to characters of plant root system and application thereof
  • SNP (single nucleotide polymorphism) site related to characters of plant root system and application thereof
  • SNP (single nucleotide polymorphism) site related to characters of plant root system and application thereof

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Experimental program
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Effect test

Embodiment 2

[0058] Embodiment 2, the application of SNP

[0059] 29 non-411 offspring varieties and 2 Jing 411 derived varieties were selected. Among them, 2 derivative varieties of Jing 411 were the control group, including Zhongmai 175 and CA9722; 29 non-411 offspring varieties were the test group, including Bima 1, Han 6172, Huai Mai 18, Huai Mai 20, Jimai 19 , Jimai 21, Jingshuang 16, Jimai 22, Jinmai 45, Liangxing 66, Linhan 2, Liangxing 99, Shaanxi 354, Mianyang 26, Yannong 19, Wanmai 38, Wennong 14, Xinong 979 , Xiaoyan 54, Yangmai 9, Zhongyou 9507, Yumai 63, Zhongmai 895, Zhoumai 16, Zhoumai 18, Zhoumai 22, Zhoumai 31, Zhoumai 32 and Jimai 20.

[0060] 1. Detection of root traits of different varieties of wheat at seedling stage

[0061] The identification of root traits of wheat varieties at seedling stage was carried out in the greenhouse of Institute of Genetics and Developmental Biology, Chinese Academy of Sciences in the winter of 2012. Choose 50 full and uniform seeds for...

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Abstract

The invention discloses an SNP (single nucleotide polymorphism) site relates to characters of a plant root system and application thereof. The SNP site related to the characters of the plant root system is a 55th nucleotide from a tail end 5' in SEQ ID No.4, and the 55th nucleotide is C and / or G; a DNA segment shown in SEQ ID No.4 is at a position 19.3cM on a wheat chromosome 1BS. The invention provides a new root system major gene site QRt.caas-1BS and an auxiliary excellent wheat root system screening SNP site; wheat with excellent root system characters can be screened by utilizing the SNP site, so that the SNP site plays an important role in wheat root system related breeding.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to an SPN site related to plant root traits and its application. Background technique [0002] Breeding high-yield, wide-ranging, and water-fertilizer-efficient new wheat varieties requires not only excellent agronomic traits, but also a stable and well-developed root system and long-lasting root vitality. Due to the complexity of the wheat root growth environment and the limitations of research methods and means, the genetic research on root traits lags behind that of aboveground tissues. Therefore, breeders still lack the knowledge and understanding of the genetic rules of wheat root traits, which limits This hinders the genetic research process of variety roots and restricts the cultivation of new varieties with high yield and wide adaptability. With the deepening of researchers' understanding of the importance of the root system, some methods of root system research have been devel...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11C12Q1/68
Inventor 何中虎肖永贵夏先春陈新民李思敏
Owner INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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