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Bighead atractylodes rhizome regeneration system for directly differentiating adventitious buds by utilizing hypocotyls and radicles

A technology of Atractylodes macrocephala and radicle, applied in the field of Atractylodes atractylodes regeneration system, can solve the problems of long artificial propagation time, poor quality stability and low frequency of regenerated plants, and achieve the goal of promoting gastrointestinal motility, regulating gastrointestinal dysfunction and improving immunity Effect

Active Publication Date: 2017-05-31
HEBEI UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to provide a regeneration system of Atractylodes Rhizoma Atractylodes Rhizoma by using hypocotyls and radicles to directly differentiate adventitious buds, so as to solve the problems of long time and low frequency of artificial propagation of Atractylodes Rhizoma, and poor quality stability of regenerated plants

Method used

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  • Bighead atractylodes rhizome regeneration system for directly differentiating adventitious buds by utilizing hypocotyls and radicles
  • Bighead atractylodes rhizome regeneration system for directly differentiating adventitious buds by utilizing hypocotyls and radicles
  • Bighead atractylodes rhizome regeneration system for directly differentiating adventitious buds by utilizing hypocotyls and radicles

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Effect test

Embodiment 1

[0024] (1) Obtaining sterile vaccines. Select large and plump Atractylodes macrocephala seeds, rinse them with running water, soak them in alcohol with a concentration of 75% by mass for 1 min, and then disinfect them. A two-step method was used for disinfection: before removing the seed coat, soak the seeds with mercuric chloride with a concentration of 0.1% by mass for 10 minutes, rinse with sterile water for 3-5 times, soak in sterile water for 6 hours, and then peel off the seed coat; Disinfect with 1.5% NaClO for 10 min and rinse with sterile water 3-5 times. The sterilized seeds were inoculated in MS medium without adding any growth regulator, cultivated in a dark place at 25±2°C until the seeds germinated, and then transferred to the light to continue culturing, culture temperature: 25±2°C, light time: 14h / d, light intensity: 3500 lx.

[0025] (2) Continue culturing for 1 week after Atractylodes macrocephala seeds germinate, take out when the plant height reaches 2-3c...

Embodiment 2

[0029] The method is the same as in Example 1, except that the bud induction medium in step (2) is changed: 1.0 mg of TDZ, 30 g of sucrose and 8 g of agar are added to 1 L of MS medium, and the pH value is adjusted to 5.8, at 121 °C Autoclave for 15 min to prepare the medium.

Embodiment 3

[0031] The method is the same as in Example 1, except that the bud induction medium in step (2) is changed: 0.5 mg of TDZ, 0.2 mg of NAA, 30 g of sucrose and 8 g of agar are added to 1 L of MS medium, and the pH value is adjusted to 5.8 , 121 ° C autoclave for 15 min to prepare the culture medium.

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Abstract

The invention discloses a bighead atractylodes rhizome regeneration system for directly differentiating adventitious buds by utilizing hypocotyls and radicles. The bighead atractylodes rhizome regeneration system comprises the following steps: washing and disinfecting bighead atractylodes rhizome seeds; inoculating the disinfected bighead atractylodes rhizome seeds into an MS culture medium; culturing in a dark place until the seeds are germinated; transferring the seeds and continually culturing under light; cutting the radicles and the hypocotyls, cutting into small blocks and taking the small blocks as explants; inoculating the explants into a bud induction culture medium and inducing and culturing; differentiating until green buds grow out; cutting off the green buds and transplanting the green buds into a rooting culture medium for culturing; inducing for rooting; transplanting regenerated plants with roots so as to obtain bighead atractylodes rhizome plants. According to the regeneration system, provided by the invention, the proliferation frequency of bighead atractylodes rhizome is greatly improved, the production cost is saved and the propagation efficiency is improved; technical supports are provided for large-scale production of medical bighead atractylodes rhizome; for the organogenesis which does not subject to calluses, the genetic stability of the plants is higher; quality guarantees are provided for bighead atractylodes rhizome quality improvement, industrial seedling culturing and virus elimination which are realized by utilizing a plant gene engineering technology.

Description

technical field [0001] The invention relates to the propagation technology of medicinal materials, in particular to a regeneration system of Atractylodes Rhizoma Atractylodes Rhizoma Rhizoma Rhizoma Atractylodes Rhizoma Atractylodes Rhizoma Atractylodes Rhizoma Atractylodes Rhizoma Atractylodes Rhizome for direct differentiation of adventitious buds by utilizing hypocotyls and radicles. Background technique [0002] Atractylodes ( Atractylodes macrocephala Koidz. ) is a perennial herbaceous plant belonging to the genus Atractylodis of Asteraceae. It has dry rhizomes, fragrant air, sweet and bitter taste, and warm nature. It is mainly produced in Zhejiang, Hunan, Hubei and Anhui in my country (Chinese Pharmacopoeia 2010). Jia, especially called "Yu Shu". "Shen Nong's Materia Medica" contains the medicinal value of Atractylodes macrocephala for the first time, and it is listed as top grade. Atractylodes macrocephala is one of the traditional Chinese medicines in my country, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 梁玉玲鲜于梁艳刘雯雯
Owner HEBEI UNIVERSITY
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