Rapid Propagation Method of High-quality Seedlings of Lycium barbarum in Vitro and Micro-cutting

A technology of Qaidam Lycium barbarum and micro-cutting, which is applied in the field of plant biology, can solve the problems of infected seedlings, large area and low reproduction efficiency, and achieves the effects of high proliferation frequency, easy implementation and simple operation.

Inactive Publication Date: 2016-06-15
CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In terms of production, wolfberry is mainly propagated by asexual cuttings, which occupies a large area and has low reproductive efficiency.
Long-term asexual cutting propagation causes the virus to accumulate in the plant mother, and the cuttings will infect the seedlings, which is not conducive to the breeding of high-quality seedlings

Method used

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  • Rapid Propagation Method of High-quality Seedlings of Lycium barbarum in Vitro and Micro-cutting
  • Rapid Propagation Method of High-quality Seedlings of Lycium barbarum in Vitro and Micro-cutting

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1 The method for rapid propagation of Qaidam Lycium barbarum high-quality seedlings in vitro by microcutting includes the following steps:

[0025] ⑴ After separating the obtained Qaidam Lycium barbarum adventitious seedlings per plant, transfer them to the first-level seedling growth medium at an inoculum of 7 plants / bottle, at a temperature of 20°C and a light intensity of 3000 Lux, light time Cultivation is carried out under 16h / day light conditions, and the first-level seedlings will be obtained after 10 days.

[0026] Among them: the first-level seedling growth medium refers to each liter of MS basic medium supplemented with 2.0mg 6-BA, 100mg hydrolyzed casein, 1mgVc, 2mg sodium citrate, 20.0g sucrose, 8g agar powder, and the pH value is 5.0 Medium.

[0027] ⑵ Cut the first-level seedlings into 2~3cm stem segments with 1~3 leaves, and transfer them to the second-level seedling medium according to the inoculation amount of 8 segments / bottle, at a temperature of 20...

Embodiment 2

[0033] Example 2 The method for rapid propagation of Qaidam Lycium barbarum high-quality seedlings in vitro by microcutting includes the following steps:

[0034] ⑴ After separating the obtained Qaidam Lycium barbarum adventitious seedlings from individual plants, transfer them to the first-level seedling growth medium at an inoculum of 10 plants / bottle, at a temperature of 25°C and a light intensity of 10000 Lux, light time Cultivation is carried out under the light condition of 12h / day, and the first-level seedlings will be obtained after 14 days.

[0035] Among them: the primary seedling growth medium refers to the culture with 0.005mgKT, 300mg hydrolyzed casein, 4mgVc, 8mg sodium citrate, 40.0g sucrose, 4.5g agar powder and pH 6 per liter of MS basic medium base.

[0036] ⑵ Cut the first-level seedlings into 2~3cm stem segments with 1~3 leaves, and transfer them to the second-level seedling culture medium according to the inoculation amount of 10 segments / bottle, at a temperatur...

Embodiment 3

[0042] Example 3 The method for rapid propagation of Qaidam Lycium barbarum high-quality seedlings in vitro by microcutting includes the following steps:

[0043] ⑴ After individual plant isolation of the obtained Qaidam Lycium barbarum adventitious seedlings, they are transferred to the first-level seedling growth medium at an inoculum of 8 plants / bottle, at a temperature of 23°C and a light intensity of 6000 Lux, light time Cultivation is carried out under the light condition of 14h / day, and the first-level seedlings will be obtained after 12 days.

[0044] Among them: the primary seedling growth medium refers to the medium with 1.0mg ZT, 200mg hydrolyzed casein, 2mgVc, 5mg sodium citrate, 60.0g sucrose, 3g agar powder and pH 6.5 per liter of MS basic medium .

[0045] ⑵ Cut the first-level seedlings into 2~3cm stem segments with 1~3 leaves, and transfer them to the second-level seedling culture medium according to the inoculation amount of 9 segments / bottle. The temperature is 23...

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Abstract

The invention relates to an in vitro microcuttage rapid-propagation method for high-quality Qaidam wolfberry seedlings. The method comprises the following steps: (1) after individually separating the obtained Qaidam wolfberry plants without final singling, transferring to a first-level seedling growth culture medium to be cultured, and obtain first-level seedlings after 10 to 14 days; (2) cutting the first-level seedlings into sections and then transferring to a second-level seedling culture medium to be cultured, and obtaining second-level seedlings after 10 to 14 days; (3) cutting the second-level seedlings growing to 5 to 7cm into stems with leaves and then transferring to a third-level seedling culture medium to be cultured, and obtaining third-level seedlings after 10 to 14 days; (4) cutting the third-level seedlings growing to 5 to 7cm into sections and then inoculating into a rooting culture medium to be cultured, and obtaining microcuttage rapid-propagation seedlings after 10 to 14 days. According to the method provided by the invention, a large number of virus-free high-quality seedlings can be obtained in the short term, and the large-scale production can be carried out.

Description

Technical field [0001] The invention relates to the field of plant biotechnology, in particular to a method for rapid propagation of Qaidam wolfberry high-quality seedlings in vitro by microcutting. Background technique [0002] Lycium barbarum Mill. is a perennial deciduous shrub of the Lycium genus Lycium of the Solanaceae family. The fruit is called Lycium barbarum, and the root bark is called Diguh. Both can be used as medicine. The tender stems and leaves can be used as vegetables. The Qaidam Basin is rich in wolfberry resources and is an emerging producing area of ​​wolfberry. The Qaidam wolfberry produced is one of the original plants of the famous local medicinal materials in Qinghai. "Chai Damu" is also one of the best quality wolfberries in China. [0003] In the production, wolfberry mainly relies on asexual cuttings to breed, which covers a large area and low reproduction efficiency. Long-term asexual cutting propagation causes the virus to accumulate in the plant mate...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 王莉李毅任刚胡延萍石琳王溪王建科冯承彬唐楠
Owner CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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