Method of promoting generation of lateral buds of Heveabrasiliensis by trans-AtWUS (Arabidopsisthaliana WUSCHEL) gene

Abstract

The invention discloses a method of promoting generation of lateral buds of a Heveabrasiliensis by a trans-AtWUS (Arabidopsisthaliana WUSCHEL) gene. Genetic transformation is carried out on a fragile embryonic callus of the Heveabrasiliensis by adopting the AtWUS gene to obtain a transgenetic material to promote generation of the lateral buds of the Heveabrasiliensis and to form lateral branches, so that the function of the AtWUS gene is verified, which shows that the WUS gene has an important effect on generation of the lateral buds and formation of the lateral branches of the Heveabrasiliensis. The lateral buds and lateral branches obtained have actual application potential. By means of the agrobacterium-mediated method, a plant expression vector is established to transfer the AtWUS gene to the callus of the Heveabrasiliensis to obtain the transgenetic material so as to promote somatic embryogenesis or organogenesis of the Heveabrasiliensis and promote the ability of forming a regeneration plant or a budling according to the property that the plant stem cell determinant gene WUS has a key effect on the meristem of a plant and promotes somatic embryogenesis and / or organogenesis. The method serves application and production from a novel path of genetic engineering of the Heveabrasiliensis.

Description

technical field [0001] The invention belongs to the technical field of plant genetic engineering, and in particular relates to a method for promoting the occurrence of lateral buds of rubber trees by transferring the AtWUS gene. Background technique [0002] Plant somatic embryogenesis and organogenesis are extremely complex processes. Recent studies have shown that many genes are involved in the regulation of plant somatic embryogenesis, among which WUS (WUSCHEL) gene is a typical stem cell determining gene. In 1996, Laux et al identified WUS using EMS mutagenesis technology, and found that its gene mutants affected the development of apical meristem and floral meristem, thus inferring that WUS plays a key role in apical meristem and floral meristem sex and maintain the unity of structure and function. Mayer et al found that WUS encodes 291 amino acids and is a new subtype of homeodomain protein. During the development of plant embryos, WUS gene is expressed in the centr...

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Problems solved by technology

[0005] The purpose of the embodiments of the present invention is to provide a method for promoting the occurrence of lateral buds of rubber trees by transducing the AtWUS gene, so as to solve the problem of difficult occurrence of lateral buds in the tissue culture process of rubber trees, and the obtained lateral buds and lateral branches have the potential to be practically applied to grafting

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