58results about How to "Improve transcription efficiency" patented technology

The present invention concerns a method for modulating the enzymatic activity of an enzyme, wherein said enzyme interacts with BMP, said method comprising the step of administering or inducing Hsp70, or a functional fragment or variant thereof, in a form suitable for allowing interaction between BMP and Hsp70, or said functional fragment or variant thereof, and thereby modulating the enzymatic activity of an enzyme interacting with BMP.

The invention discloses a service data processing method and device, a computer device and a storage medium. The method comprises the following steps of: creating at least one original splicing function, wherein each original splicing function comprises a splicing type; obtaining business data requirements including target type, target parameter, target source and target condition; querying the database according to the target type, and determining the original splicing function corresponding to the target type as the target splicing function; adopting target splicing function to splice targetparameters, target source and target condition to generate a target SQL instruction; according to the target SQL instruction, processing the source table corresponding to the target source in the database to obtain the target data. The business data processing method can automatically convert the business data into SQL instructions according to the business data requirements, and process the dataaccording to the SQL instructions, so as to improve the data processing efficiency and save the human cost.

The present invention relates in one embodiment to PAPP-A exosite(s) interactors such as antibodies which bind to a region comprising LNR3 of PAPP-A and efficiently inhibit proteolysis of IGFBP-4, but not -5. The region comprising LNR3 represents a substrate binding exosite, which can be targeted for selective proteolytic inhibition. Accordingly, the present invention relates in one embodiment to differential inhibition of natural protease substrates by exosite targeting.

The invention relates to an RT-PCR method for quantitatively detecting miRNA. The method is characterized in that Poly (A) tail adding and reverse transcription of miRNA are performed in the same reaction system, and S-Poly (T) primers are used to perform the reverse transcription of miRNA. The method has the advantages that in an S-Poly (T) Plus method, the Poly (A) tail adding and reverse transcription of miRNA are simultaneously performed in the same reaction system, simple operation is achieved, time is shortened, time for synthesizing cDNA is at least reduced by 0.5 hour, and high reverse transcription efficiency is achieved; an S/P miRsol RNA method and the S-Poly (T) Plus method are combined to build a technical system which is quite sensitive, efficient, simple, fast and cheap and capable of achieving extraction and detection; the technical system is especially suitable for detecting miRNA in biological fluid samples with low miRNA abundance.

The invention provides a lentivirus recombinant expression vector/a recombinant lentivirus, as well as application, a host cell and a preparing method thereof. The encoding gene of an estrogen related receptor (ERR) protein can be inserted in a host gene group by the lentivirus recombinant expression vector/the recombinant lentivirus which is provided by the invention through a gene recombinant so as to continuously and stably express the ERR. The ERR can be permanently and stably expressed by the host cell which is provided by the invention, and sufficient high-quality virus liquid can be provided for animal in-vivo experiments. Besides, the lentivirus recombinant expression vector/the recombinant lentivirus which is provided by the invention has a wide host range, can infect various cells, such as nerve cells, muscle cells, liver cells, tumor cells and endothelial cells and has a very wide application range.

The invention discloses a single-subunit RNA polymerase and its purification method and application in RNA synthesis. The single-subunit RNA polymerase is a single-subunit RNA polymerase derived froma phi KMV phage or an other phage single-subunit RNA polymerase with a protein sequence which is 25% or more homologous to the single-subunit RNA polymerase derived from the phi KMV phage, contains acharacteristic amino acid sequence shown in the formula of SEQ ID NO. 1 in the sequence table and has the total amino acid sequence number of 800 and 830. The research result shows that the RNA polymerase has a long-distance relationship with the existing RNA tool enzyme and high transcription efficiency. Compared with the existing T7 RNA polymerase and Syn5 RNA polymerase, the single-subunit RNApolymerase solves the problem that the synthesis of RNA rich in a stable and high-level structure from the T7 RNA polymerase and Syn5 RNA polymerase produces complex products. The single-subunit RNA polymerase produces same transcription products.

The invention discloses an expression element composed of an aspergillus niger and an expression vector composed of the expression element, and a recombined aspergillus niger and a construction method and an application thereof. The expression element is prepared from an aspergillus niger transcription elongation factor gene promoter and an aspergillus niger transcription elongation factor gene terminator, wherein the aspergillus niger transcription elongation factor gene promoter is composed of 1490, 990 or 690 basic groups of which sequence is as shown in SEQID NO.1, SEQID NO.2 and SEQID NO.3; the aspergillus niger transcription elongation factor gene terminator is composed of 1022 basic groups of which sequence is as shown in SEQID NO.5. The expression element can start protein expression without adding an inductor, so that the production cost is saved; the expression element can be used for preparation of pharmaceutical protein of eukaryotes and microbial sources and enzymes for industrial purposes.

The invention provides a meter reading method applied to a meter reading terminal. The method comprises the steps of selecting an identifier of a to-be-read electric meter from electric meter identifiers of a plurality of electric meters, fed back by a concentrator; and sending a read data acquisition instruction to the concentrator according to the identifier of the to-be-read electric meter, andreceiving read data fed back by the concentrator. The invention further provides a meter reading method applied to the concentrator. The method comprises the steps of sending the electric meter identifiers of the plurality of the electric meters associated with the concentrator to the meter reading terminal; and when the read data acquisition instruction sent by the meter reading terminal is received, acquiring the read data according to the read data acquisition instruction, and sending the read data to the meter reading terminal. Corresponding to the meter reading method, the invention further provides the meter reading terminal, the concentrator and a computer readable storage medium. By means of the technical scheme, the efficiency of electric meter reading can be improved.

The invention relates to the field of biotechnology and discloses a high-proliferation specific recombination eucaryotic cell promoter PCNATAI; an eucaryotic cell operating system PCNATAI-LoxP-stop-LoxP taking a PCNATAI as a core transcription initiator and a LoxP-stop-LoxP as a transcriptional repression element; and a set of general scheme for eliminating the repression effect of the LoxP-stop-LoxP to the transcription process of the PCNATAI promoter through a Cre recombinase provided by a tumor cell specific dependent model trans form and enabling the downstream exogenous tumor suppressor gene or the suicide gene to be effectively and specifically expressed in the malignant tumor cell in a tumor cell specific pattern and a proliferation specific pattern. The scheme not only can remarkably improve the tumor inhibition rate during the gene therapy of the malignant tumor or the expression efficiency of the suicide gene in the malignant tumor cell, but also can ensure the security of the gene therapy.

The invention discloses establishment of a self-activated Gal4/UAS system expression cassette capable of improving gene expression. The expression cassette with good improvement effect is obtained byoptimizing the number of UAS and the types of GAL 4 activation/binding domains; the expression cassette is started by the strong promoter CMV of the expression cassette and improves transcription andtranslation efficiency in virtue of a Gal4/UAS system, so the effects of automatic activation and cascade amplification are obtained; high improvement effect is maintained in 5 to 10 days after transfection, and highest expression activity is obtained in the eighth day, so it is proved that the expression cassette can improve the stability of gene expression; and improvement effect is about 9 times better in CHO cells specially producing recombinant proteins. Compared with conventional gene expression cassettes, the expression cassette provided by the invention is simple in structure and substantial in improvement effect, and provides an approach and novel method for non-virus transfection approaches of gene therapy and enhancement of the expression of target genes in production of recombinant proteins.

The invention provides a negative charge toner, method of manufacturing the same and image forming device using the same. The said toner comprises: toner agglomerate, hydrophobic additive with negative charge and with less work function than the toner agglomerate; hydrophobic additive with positive charge and with less work function than the toner agglomerate and for treating the surface of the toner agglomerate using material with positive charge. Therefore, the amount of fog toner on non-image portions is reduced, the transfer efficiency is further improved, the charging property is further stabilized, and the production of reverse transfer toner is further inhibited.

The present invention relates to a polypeptide exhibiting a protease inhibitory activity and uses of said polypeptide in methods for inhibiting, directly or indirectly, one or more proteases of the blood clotting cascade. The invention also relates to use of said polypeptide as a pharmaceutical e.g. for prophylactic or ameliorating treatment of blood clots. In addition the invention comprises methods for production of said polypeptide.

The invention relates to an OCR technology-based different types of printed document transcription method. The method comprises the following steps of: A, converting different types of printed documents into pictures with the same format through a picture conversion tool; B, carrying out line projection on the picture, and segmenting and preprocessing text lines in the picture through an OCR technology; C, performing character recognition on all the text lines segmented in the step B through an OCR technology; and D, combining the recognized characters into a complete document according to the segmentation sequence of the text lines. According to the OCR technology-based different types of printed document transcription method, character transcription can be conducted on various different types of printed documents in a unified mode, multiple transcription toolkits are not needed, the transcription efficiency and the transcription convenience degree are greatly improved, and meanwhile the transcription accuracy is also obviously improved.

The invention relates to gene optimization of cholesterol dehydrogenase and high-efficiency expression thereof, belonging to the field of production of protein by utilizing recombinant DNA (deoxyribonucleic acid) technology. Particularly, the invention provides an optimized DNA sequence of cholesterol 7,8-dehydrogenase, which is suitable for a Escherichia coli expression system or a pichia pastoris expression system, and research on construction of engineering bacteria and the expression optimization technology of the cholesterol 7,8-dehydrogenase. The optimized gene is transferred into Escherichia coli and pichia pastoris to be expressed, and compared with the expression quantity of a non-optimized gene, the expression quantity of the optimized gene in the Escherichia coli and pichia pastoris is obviously improved.

We have developed a versatile plant viral vector system based on Alternanthera mosaic virus (AltMV), suitable for infection by agroinfiltration or in vivo T7 transcripts from the same clone; agroinfection is enhanced by coinfiltration of a T7 RNA polymerase construct. Variants adapted for efficient protein expression, or for virus-induced gene silencing (VIGS), are based on a specific amino acid substitution (L88P) in the triple gene block 1 (TGB1) protein affecting RNA silencing suppression. A bipartite delivery system developed for AltMV delivers replicase (RdRp) functions separately from movement and encapsidation (TGB and coat protein, CP) functions by agroinfiltration, resulting in precise recombination of RdRp and TGB-CP constructs in planta. The bipartite delivery system has potential for high throughput protein expression or VIGS with the appropriate TGB1 variant, for hosts including Nicotiana benthamiana and Arabidopsis thaliana. Equivalent TGB1 substitutions in other potexviruses also reduced RNA silencing suppression, demonstrated with Potato virus X.

The invention discloses a method and device for transmitting and forwarding two-dimensional code information. The method comprises the steps that a website server receives a user request; According tothe request, the website server randomly generates verification information and a two-dimensional code with the same content as the verification information, and sends the verification information and the two-dimensional code to a mobile intelligent device or an intelligent terminal device together; The mobile intelligent device receives the verification information and the two-dimensional code and displays the verification information and the two-dimensional code in a screen at the same time; a verification code is input into the intelligent terminal device or the two-dimensional code is scanned through the intelligent terminal device; The intelligent terminal device sends the input verification information or the identified two-dimensional code to the website server for verification; Wherein the two-dimensional code further comprises a website link of the website server and fonts and format information in the verification information. In this way, the information transcription efficiency can be improved through code scanning.

The invention relates to a method for generating a transgenic organism. The invention also relates to a method for detecting and characterizing a genetic mutation in a transgenic organism. The invention further relates to a method for isolating a gene which is correlated with a phenotypic characteristic in a transgenic animal. The invention further relates to a method for isolating an exon in a transgenic animal. The invention also relates to a method for modulating the expression of a gene in an organism.

Novel zacrp3x2 polypeptides, polynucleotides encoding the polypeptides, and related compositions and methods are disclosed. Also disclosed are antibodies to the zacrp3x2 protein or fragments thereof.