Method of establishing polyploiding system for Dongzao date

A polyploid, winter jujube technology, applied in the field of agricultural biotechnology or plant cell engineering

Inactive Publication Date: 2007-11-14
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Jiang Hongen et al. (2004) used winter jujube, Linyi pear jujube and capsicum jujube as test materials, and colchicine was used as a mutagen to induce polyploidy, but only one purified tetraploid was obtained from Linyi pear jujube. ploidy plant

Method used

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  • Method of establishing polyploiding system for Dongzao date

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Shoot Tip Culture of Zhanhua Dongzao

[0042] Zhanhua Dongzao is one of the winter jujube varieties with the best fresh food quality, and it is mainly produced in the Bohai Bay area of ​​our province. The dormant buds of Zhanhuadongzao germinate in a constant temperature incubator at 28°C and 70% humidity. The germinated shoots were cut into single-bud stem segments, sterilized with 70% alcohol for 30 seconds, sterilized with 0.1% mercuric chloride for 8 minutes, rinsed with sterile water for 3-4 times, and then inoculated in 1 mg L -1 GA 3 and 0.2 mg L -1 On 6-BA MS solid medium, subculture once every 35 days. Tissue culture seedlings can grow more than 2cm in 10-15 days, and the cells at the growth point are in a state of vigorous division.

[0043] Colchicine (Sigma, USA) was added in the -1 GA 3 and 0.2mg L -1 BA in MS liquid medium, the final concentration was 0.1%. The shoot tips with vigorous cell division were placed in MS liquid medium containing 0....

Embodiment 2

[0055] Concrete method is as embodiment 1, wherein, stem elongation culture medium hormone concentration is 1.5mg L -1 GA 3 and 0.3mg L -1 6-BA, the concentration of colchicine was 0.1%, and the treatment time was 72 hours, and the tetraploid plants were induced, and the hormone concentration of the rooting medium was 0.3 mg L -1 IAA and 0.4mgL -1 IBA.

Embodiment 3

[0057] Concrete method is as embodiment 1, wherein, stem elongation culture medium hormone concentration is 2mg L -1 GA 3 and 0.4mg L -1 6-BA, the concentration of colchicine was 0.1%, and the treatment time was 48 hours, and the tetraploid plants were induced, and the hormone concentration of the rooting medium was 0.3 mg L -1 IAA and 0.6 mg L -1 IBA.

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Abstract

The method of establishing polyploiding system for Dongzao date includes culturing Dongzao date stem apex and leaf with hearty cell division; processing Dongzao date stem apex and leaf with colchicines for chromosome doubling; seedling rooting and transplanting; grafting of tetraploid plant; applying the characteristic of tetraploid plant and other steps. The present invention can overcome genotype restriction, has high efficiency of obtaining homogenous polyploid and has important significance in genetic improvement of date variety.

Description

technical field [0001] The invention relates to a method for establishing a polyploid induction system of winter jujube, which belongs to the field of agricultural biotechnology or plant cell engineering. Background technique [0002] my country is the origin and cultivation center of jujube tree, and its germplasm resources are very rich. Winter jujube (Ziziphus juj uba Mill.) is a plant of the genus Jujube in the family Rhamnaceae. It is named after the fruit ripens after the Cold Dew Festival. Dongzao has a unique flavor and high yield. It is a fresh cultivar with good quality and has important economic value in fruit tree production and trade. Zhanhua Dongzao is one of the varieties with the best fresh food quality in Dongzao. It is mainly produced in the Bohai Bay area of ​​Shandong Province. It is suitable for growth in low-to-medium salinity land and has high economic value. However, Zhanhua Dongzao has disadvantages such as small fruit, large core, thin peel, and p...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G17/00A01H1/08A01G1/06
Inventor 谷晓峰张举仁张可炜杨爱芳尹小燕
Owner SHANDONG UNIV
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