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Rapid propagation method of tissue culture of female and male plants of Zhejiang hemsleya amabilis

A technology of snow gall female and snow gall, applied in the biological field, can solve the problems of not knowing the sex of the plant, easy yellowing, etc., and achieve the effect of maintaining genetic stability

Active Publication Date: 2019-09-10
ZHEJIANG UNIV +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the inventor found in the research that there are still some shortcomings in the method reported by the predecessors, such as Zhejiang snow gall tissue culture seedlings are prone to yellowing on MS medium, and the above-mentioned studies do not know the sex of the plant

Method used

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  • Rapid propagation method of tissue culture of female and male plants of Zhejiang hemsleya amabilis
  • Rapid propagation method of tissue culture of female and male plants of Zhejiang hemsleya amabilis
  • Rapid propagation method of tissue culture of female and male plants of Zhejiang hemsleya amabilis

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Embodiment 1

[0104] Embodiment 1, a kind of tissue culture rapid propagation method of Zhejiang Xuedan female and male strains, carries out the following steps successively:

[0105] (1), drawing materials:

[0106] During the flowering period of Zhejiang Snow Gallbladder (May to June), select the female and male plants of Zhejiang Snow Gallbladder, remove the stems and leaves of the aboveground part, dig out the tubers and plant them in flower pots, and wait for new stems to be drawn out from the tubers After the paragraph, get the materials.

[0107] Specifically: bury the tubers in the matrix of river sand: peat soil: vermiculite (1:1:1 weight ratio), wait for the tubers of known gender to re-grow young stem segments, and the length of the stem segments reaches 50-100cm , take this long stem segment as an explant.

[0108] (2), disinfection of stem section:

[0109] When the stem section obtained in step (1) is a short stem section with only 1 to 2 nodes, directly use the short stem ...

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Abstract

The invention discloses a rapid propagation method of tissue culture of female and male plants of Zhejiang hemsleya amabilis. The stem segments of the female and male plants of Zhejiang hemsleya amabilis of which the sex is known are utilized as explants, and the method further comprises the following steps: disinfection of the stem segments, starting cultivation of axillary buds, enrichment culture / rapid augmentation of the stem segments of aseptic seedlings, induction of blade callus, and differentiation culture, rooting culture and transplantation of adventitious buds. The method successively realizes the rapid propagation of the tissue culture of the female and male plants of Zhejiang hemsleya amabilis, can rapidly cultivate a large quantity of test-tube plantlets, of which the sex isknown, within a short period of time, provides a feasible propagation mode for the recovery of an extremely small population, and further provides a feasible development project for planting traditional Chinese medicine GAP simultaneously.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for tissue culture rapid propagation of female and male strains of Zhejiang Snow Gallbladder. Background technique [0002] Plants are one of the natural treasure houses of medicines. Some compounds that plants produce for their own defense are exploited by humans to prevent and treat diseases. Due to habitat destruction and predatory excavation, the content of many traditional Chinese medicine resources has declined or even been exhausted, and some species are on the verge of extinction (Huang Luqi, 2001). Plant tissue culture technology has great potential for rapid plant propagation, protection and enhancement of the level and content of useful secondary metabolites to meet drug needs and reduce in situ harvesting (Bapat et al., 2008). The application of this technology in the protection of medicinal plant resources, especially the protection of endangered medicinal pla...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 姜维梅姚健何晨琪邱英雄雷祖培郑方东
Owner ZHEJIANG UNIV
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