Method for culturing tissue of 'Zhongzhen No.1'

A culture medium and pre-cultivation technology, applied in the field of plant tissue culture, can solve the problems of Weizhongzhen No. 1 in establishing a high-efficiency tissue culture rapid propagation technology system, tissue culture browning, tissue culture rapid propagation difficulties, etc., to solve pollution and Browning problem, the effect of ensuring genetic stability

Active Publication Date: 2010-12-29
CHINA AGRI UNIV
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Problems solved by technology

[0007] Zhongzhen No. 1 belongs to woody plants, and its browning is a serious problem in tissue culture, and the degree of browning increases with the age of seedlings, which brings great challenges to the

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  • Method for culturing tissue of 'Zhongzhen No.1'

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Example Embodiment

[0041] Example 1. Preparation of regenerated plants of Zhongzhen No. 1 and its observation statistics and analysis

[0042] 1. Obtaining explants

[0043] 1) Pre-culture to get pre-cultured seedlings

[0044] Zhongzhen No. 1 (this material has been approved by the Beijing Forestry Variety Approval Committee as an improved forest tree species on December 30, 2009, the number of the improved species is Jing S-SV-MX-015-2009, and this information is available from January 2010. The "2009 Beijing Forest Variety Approval Announcement" issued on 16th, the public can obtain the seeds from China Agricultural University for stratification treatment. When the seeds begin to germinate until the radicle grows to more than 2cm, they should be carefully Insert the pre-cultivation medium, put it in the light incubator, control the temperature at 23℃, the light intensity is 2000-2500Lx, and cultivate under the condition of 16h / 8h photoperiod (light / dark). After the seed coat is removed, the precult...

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Abstract

The invention discloses a method for culturing tissue of 'Zhongzhen No.1'. The method comprises the following steps of: performing sterile pre-culture on 'Zhongzhen No.1' seeds to obtain sterile seedlings; and then performing primary culture on stem tips of the sterile seedlings to obtain regenerated seedlings. Experiments show that by performing pre-culture before producing the 'Zhongzhen No.1' regenerated seedlings, the differentiation rate of the stem tip of the pre-cultured seedling reaches 87.77 percent while the differentiation rate of the stem tip of the seedling cultured in nutrient soils is only 21.07 percent. In the method of the invention, the seeds are used as explant materials, and by adopting a mode of preculture of the sterile seedlings, the genetic stability of excellent characters is guaranteed; moreover, the method of the invention has the advantages that: the antiseptic processing method is improved; and the types of suitable anti-browning materials and hormones and the concentrations of the materials are screened out, the pollution and browning problems in present researches are solved, and a 'Zhongzhen No.1' sterile cultivation system is established successfully.

Description

technical field [0001] The invention relates to the field of plant tissue culture, in particular to a method for Zhongzhen No. 1 tissue culture. Background technique [0002] Apple is the fourth largest fruit in the world after citrus, bananas, and grapes, and it is also the fruit with the largest cultivated area and the largest output in my country. my country's apple production has a long history of cultivation and rich management experience, and has formed an industrial chain of seedling breeding, production cultivation, post-harvest processing, storage and deep processing. But generally speaking, compared with developed countries, the current apple production in my country has an unreasonable tree species structure, and most of them still have extensive management, low cultivation management level, poor fruit quality, and low returns. To change the current situation of extensive planting, low harvest, high input and low output, strengthen the transformation of existing ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 韩振海许雪峰王忆张新忠孙扬吾沈隽
Owner CHINA AGRI UNIV
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