A kind of method for induction and in vitro preservation of the first generation of Smilax nigra

A technology of in vitro preservation and sarsaparilla, applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problems of difficult survival of cuttings and grafting, long dormant period of seeds, low annual tillering ability, etc., and achieve induction rate and The effect of high survival rate, prolonged subculture cycle, and low price

Inactive Publication Date: 2017-10-24
GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The breeding characteristics of Smilax sarsaparilla mainly rely on natural tillering or seed reproduction, but the annual tillering ability of each plant is low. Studies have found that the seeds of Liliaceae have a long dormant period and low germination rate, and cuttings and grafting are difficult to survive.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] (1) Disinfection of explants: drop 3 to 5 drops of detergent into a beaker with 100ml of tap water, take the young stems with buds of Smilax smilax that are free from diseases and insect pests, and cut them into 4cm long strips. Put the bud stem section in a beaker and stir it, then wipe the dirt on the surface with a soft brush, rinse it with running water for 15 minutes, then disinfect it with 75v / v% ethanol for 15 seconds in an ultra-clean workbench, rinse it once with sterile water, and then rinse it with 0.1 v / v%HgCl 2 Sterilize for 10 minutes, soak in sterile water for 3 times, about 2 minutes each time, dry the surface moisture of the explants with sterile filter paper, and obtain the explants, wherein the sterile water is distilled water sterilized by high temperature and high pressure;

[0018] (2) Induction of sterile buds: Cut the explants obtained in step (1) into 1-1.5 cm budded stem segments, inoculate them in MS starting medium, and culture them at a cult...

Embodiment 2

[0023] (1) Disinfection of explants: drop 3 to 5 drops of detergent into a beaker with 100ml of tap water, take the young stems with buds of Smilax smilax that are free from diseases and insect pests, and cut them into 4cm long strips. Put the bud stem section in a beaker and stir it, then wipe the dirt on the surface with a soft brush, rinse it with running water for 15 minutes, then disinfect it with ethanol with a volume percentage of 75v / v% in an ultra-clean workbench for 15 seconds, rinse it once with sterile water, Then use 0.1v / v% HgCl 2 Sterilize for 10 minutes, soak in sterile water for 3 times, about 2 minutes each time, dry the surface moisture of the explants with sterile filter paper, and obtain the explants, wherein the sterile water is distilled water sterilized by high temperature and high pressure;

[0024] (2) Induction of sterile buds: Cut the explants obtained in step (1) into 1-1.5 cm budded stem segments, inoculate them in MS starting medium, and culture ...

Embodiment 3

[0029] (1) Disinfection of explants: drop 3 to 5 drops of detergent into a beaker with 100ml of tap water, take the young stems with buds of Smilax smilax that are free from diseases and insect pests, and cut them into 4cm long strips. Put the bud stem section in a beaker and stir it, then wipe the dirt on the surface with a soft brush, rinse it with running water for 15 minutes, then disinfect it with ethanol with a volume percentage of 75v / v% in an ultra-clean workbench for 15 seconds, rinse it once with sterile water, Then use 0.1v / v% HgCl 2 Sterilize for 10 minutes, soak in sterile water for 3 times, about 2 minutes each time, dry the surface moisture of the explants with sterile filter paper, and obtain the explants, wherein the sterile water is distilled water sterilized by high temperature and high pressure;

[0030] (2) Induction of sterile buds: Cut the explants obtained in step (1) into 1-1.5 cm budded stem segments, inoculate them in MS starting medium, and culture ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to an initial induction and in vitro conservation method for smilax gla uco-China. The method comprises the steps of: (1) taking a young and tender smilax gla uco-China stem section with a bud as an explant and disinfecting the explant; (2) putting the disinfected explant in an MS basic culture medium to be induced to germinate; (3) putting a sterile bud in the MS basic culture medium and performing subculture, wherein the bud grows to a robust plant; (4) putting the robust plant in a rooting culture medium to be cultured to obtain an intact seedling with roots; and (5) putting the intact seedling with roots in an in vitro conservation culture medium to be cultured. By virtue of the method provided by the invention, a wild resource of smilax gla uco-China is favorably protected and excellent characters are favorably kept, a good test material for further conducting genetic breeding of the species and resource protection is provided, and the method has a wide application prospect.

Description

technical field [0001] The invention relates to a method for induction and in vitro preservation of the first generation of plants, in particular to a method for induction and in vitro preservation of the first generation of smilax smilax. Background technique [0002] Smilax glauco-china Warb. (Smilax glauco-china Warb.), also known as King Kong vine head, is a climbing shrub of Liliacea (Smilax L.), whose roots and stems are used as medicine. , heat-clearing, dampness, detoxification effect. The rhizomes are thick and short and rich in starch, which can be used to make cakes, wine or processed food. The young leaves harvested in spring and summer can be used fresh. They are rich in protein, amino acids and multi-vitamins, and have the medicinal effect of clearing away heat and detoxifying. They are rare plants with dual purposes of medicine and food. [0003] Smilax smilax is mainly distributed in Gansu (south), Shaanxi (south of Qinling Mountains), Shanxi (south), Henan...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/00A01H4/001
Inventor 韦莹韦坤华王一诺李磊缪剑华肖冬李翠李林轩
Owner GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap