A kind of method for induction and in vitro preservation of the first generation of Smilax nigra
A technology of in vitro preservation and sarsaparilla, applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problems of difficult survival of cuttings and grafting, long dormant period of seeds, low annual tillering ability, etc., and achieve induction rate and The effect of high survival rate, prolonged subculture cycle, and low price
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Embodiment 1
[0017] (1) Disinfection of explants: drop 3 to 5 drops of detergent into a beaker with 100ml of tap water, take the young stems with buds of Smilax smilax that are free from diseases and insect pests, and cut them into 4cm long strips. Put the bud stem section in a beaker and stir it, then wipe the dirt on the surface with a soft brush, rinse it with running water for 15 minutes, then disinfect it with 75v / v% ethanol for 15 seconds in an ultra-clean workbench, rinse it once with sterile water, and then rinse it with 0.1 v / v%HgCl 2 Sterilize for 10 minutes, soak in sterile water for 3 times, about 2 minutes each time, dry the surface moisture of the explants with sterile filter paper, and obtain the explants, wherein the sterile water is distilled water sterilized by high temperature and high pressure;
[0018] (2) Induction of sterile buds: Cut the explants obtained in step (1) into 1-1.5 cm budded stem segments, inoculate them in MS starting medium, and culture them at a cult...
Embodiment 2
[0023] (1) Disinfection of explants: drop 3 to 5 drops of detergent into a beaker with 100ml of tap water, take the young stems with buds of Smilax smilax that are free from diseases and insect pests, and cut them into 4cm long strips. Put the bud stem section in a beaker and stir it, then wipe the dirt on the surface with a soft brush, rinse it with running water for 15 minutes, then disinfect it with ethanol with a volume percentage of 75v / v% in an ultra-clean workbench for 15 seconds, rinse it once with sterile water, Then use 0.1v / v% HgCl 2 Sterilize for 10 minutes, soak in sterile water for 3 times, about 2 minutes each time, dry the surface moisture of the explants with sterile filter paper, and obtain the explants, wherein the sterile water is distilled water sterilized by high temperature and high pressure;
[0024] (2) Induction of sterile buds: Cut the explants obtained in step (1) into 1-1.5 cm budded stem segments, inoculate them in MS starting medium, and culture ...
Embodiment 3
[0029] (1) Disinfection of explants: drop 3 to 5 drops of detergent into a beaker with 100ml of tap water, take the young stems with buds of Smilax smilax that are free from diseases and insect pests, and cut them into 4cm long strips. Put the bud stem section in a beaker and stir it, then wipe the dirt on the surface with a soft brush, rinse it with running water for 15 minutes, then disinfect it with ethanol with a volume percentage of 75v / v% in an ultra-clean workbench for 15 seconds, rinse it once with sterile water, Then use 0.1v / v% HgCl 2 Sterilize for 10 minutes, soak in sterile water for 3 times, about 2 minutes each time, dry the surface moisture of the explants with sterile filter paper, and obtain the explants, wherein the sterile water is distilled water sterilized by high temperature and high pressure;
[0030] (2) Induction of sterile buds: Cut the explants obtained in step (1) into 1-1.5 cm budded stem segments, inoculate them in MS starting medium, and culture ...
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