Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Chinese chestnut tissue culture method and special culture medium thereof

A technology of tissue culture and culture medium, applied in horticultural methods, botanical equipment and methods, applications, etc., can solve the problems that restrict the application and development of modern biotechnology, low rooting rate of cutting propagation, less biotechnology, etc., and achieve perfect sterilization Treatment method, guaranteed genetic stability, low cost effect

Inactive Publication Date: 2008-12-31
CHINA AGRI UNIV
View PDF0 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, Chinese chestnut is extremely difficult to take root, and the rooting rate of cutting propagation is very low, and there are few researches on biotechnology, and there is no successful report on the aseptic culture system of Chinese chestnut adult tree
The lack of tissue culture technology has greatly restricted the application and development of modern biotechnology in the scientific research and production of chestnut.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Chinese chestnut tissue culture method and special culture medium thereof
  • Chinese chestnut tissue culture method and special culture medium thereof
  • Chinese chestnut tissue culture method and special culture medium thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1, the exploration of each experimental condition of Chinese chestnut culture in vitro

[0029] 1. Choice of hormone type and concentration

[0030] Three experimental groups were set up, and the screening methods were as follows:

[0031] Experiment 1: Inoculate the stem segment with axillary buds of Chinese chestnut Yanhong in a medium containing 1.0mg / L cytokinin KT and 0.1mg / L auxin IBA, at 23±2°C, relative humidity 50- 60%, light intensity 2000-2500Lx, under the conditions of 10 hours of light per day, cultivated for 30 days, observed the growth status of the stem segments, and as a result, 100% of the stem segments began to have axillary buds to germinate.

[0032] Experiment 2: Inoculate the stem segment with axillary buds of Chinese chestnut Yanhong in a medium containing 1.0 mg / L of KT and 0.1 mg / L of BA, and culture. The culture conditions are the same as those described in Experiment 1. As a result, no axillary buds germinated, and finally the st...

Embodiment 2

[0043] Embodiment 2, the in vitro culture of Chinese chestnut Yanhong

[0044] 1. Acquisition and disinfection of explant materials

[0045] Material collection: In the sunny morning from the end of April to the beginning of May in Beijing, take the spring new branches of the Chinese chestnut Yanhong adult tree, wrap the material with wet newspaper and put it in the refrigerator at 4°C for 24 hours (note: do not wash the explant material with running water at this time), and then Cut the material into about 1cm stems with axillary buds, remove the large leaves, and rinse with running water. The time of material collection is very important, and it is the decisive factor related to whether the aseptic system can be successfully established. The experiment of the present invention shows that the dormant buds in winter and the branches in the growing season are not suitable as explant materials for establishing the aseptic system.

[0046] Disinfection: On the ultra-clean workb...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for reproducing Chinese chestnuts and a special culture medium thereof. The culture medium of the invention is the culture medium obtained by adding IBA and at least one of the two hormones (KT and TDZ) in WPM culture medium; the final concentration of IBA is 0.1-1.0mg / l, and the final concentration of KT or TDZ is 0.5-1.0mg / l. The culture medium of the invention screens out the type of hormone, the type of antibiotic, the anti-browning substances, and the like, which are applicable for the growth of Chinese chestnuts, solves the problems of pollution and browning existing in the cultivation of Chinese chestnuts at present, and successfully establishes the asepsis cultivation system of the bearing trees of Chinese chestnuts. The method by using the culture medium of the invention for cultivating Chinese chestnuts has the advantages of simple operation and low cost, which is applicable for promotion and application.

Description

technical field [0001] The invention relates to a chestnut tissue culture method and a special culture medium thereof. Background technique [0002] Chestnut is an important economic crop, and the whole body is a treasure. For example, the chestnut fruit has important nutritional value and can be processed into a variety of foods; the chestnut tree is hard, straight in texture, anti-corrosion and moisture-resistant. Good material; its branches, leaves, bark, and bracts are rich in tannins, which can be used to extract baked gum; flowers can be used as a good source of honey, etc. It is precisely because chestnut has many important application values ​​and has a large market demand, so people pay more and more attention to the cultivation of its fine varieties. Especially at the beginning of the 20th century, the chestnut blight (Cryphonectria parasitica (Murr.) Barr) that occurred in the United States caused an unprecedented large-scale ecological environment disaster, and ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A01H4/00C12N5/04
Inventor 韩振海孙宁王忆许雪峰李天忠
Owner CHINA AGRI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com