Chinese chestnut tissue culture method and special culture medium thereof

A technology of tissue culture and culture medium, applied in horticultural methods, botanical equipment and methods, applications, etc., can solve the problems that restrict the application and development of modern biotechnology, low rooting rate of cutting propagation, less biotechnology, etc., and achieve perfect sterilization Treatment method, guaranteed genetic stability, low cost effect

Inactive Publication Date: 2008-12-31
CHINA AGRI UNIV
View PDF0 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, Chinese chestnut is extremely difficult to take root, and the rooting rate of cutting propagation is very low, and there are few researches on biotechnology, and there is no successful report on the aseptic

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Chinese chestnut tissue culture method and special culture medium thereof
  • Chinese chestnut tissue culture method and special culture medium thereof
  • Chinese chestnut tissue culture method and special culture medium thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0028] Example 1. Exploration of experimental conditions for in vitro culture of chestnut

[0029] 1. The choice of hormone type and concentration

[0030] Set up 3 experimental groups, the screening method is as follows:

[0031] Experiment 1: The stems with axillary buds of Chinese chestnut Yanhong were inoculated in a medium containing 1.0 mg / L cytokinin KT and 0.1 mg / L auxin IBA, at 23±2°C and a relative humidity of 50- Cultivate for 30 days under the conditions of 60%, 2000-2500Lx light intensity, and 10 hours of light per day. Observe the growth of the stems. As a result, 100% of the stems begin to have axillary buds to germinate.

[0032] Experiment 2: The stem segments with axillary buds of Chinese chestnut Yanhong were inoculated into a medium containing 1.0 mg / L of KT and 0.1 mg / L of BA, and the culture conditions were the same as those described in Experiment 1. As a result, no axillary buds germinated, and finally the stem tips withered and died.

[0033] Experiment 3:...

Example Embodiment

[0043] Example 2. In vitro culture of Chinese chestnut Yanhong

[0044] 1. Obtaining and disinfecting explant materials

[0045] Material: In the sunny morning of Beijing from the end of April to the beginning of May, take the new spring branches of the Chinese chestnut swallow red adult tree, wrap the material with wet newspaper and put it in the refrigerator at 4℃ for 24h (note: do not rinse the explant material with running water at this time), and then Cut the material into about 1cm stems with axillary buds, remove the large leaves, and rinse with running water. The collection time is very important, and it is a decisive factor related to the successful establishment of the aseptic system. The present invention shows through experiments that neither the dormant buds in winter nor the shoots in the growing season are suitable as explant materials for establishing a sterile system.

[0046] Disinfection: On an ultra-clean workbench, first soak the stems in 75% ethanol for 30s, ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a method for reproducing Chinese chestnuts and a special culture medium thereof. The culture medium of the invention is the culture medium obtained by adding IBA and at least one of the two hormones (KT and TDZ) in WPM culture medium; the final concentration of IBA is 0.1-1.0mg/l, and the final concentration of KT or TDZ is 0.5-1.0mg/l. The culture medium of the invention screens out the type of hormone, the type of antibiotic, the anti-browning substances, and the like, which are applicable for the growth of Chinese chestnuts, solves the problems of pollution and browning existing in the cultivation of Chinese chestnuts at present, and successfully establishes the asepsis cultivation system of the bearing trees of Chinese chestnuts. The method by using the culture medium of the invention for cultivating Chinese chestnuts has the advantages of simple operation and low cost, which is applicable for promotion and application.

Description

technical field [0001] The invention relates to a chestnut tissue culture method and a special culture medium thereof. Background technique [0002] Chestnut is an important economic crop, and the whole body is a treasure. For example, the chestnut fruit has important nutritional value and can be processed into a variety of foods; the chestnut tree is hard, straight in texture, anti-corrosion and moisture-resistant. Good material; its branches, leaves, bark, and bracts are rich in tannins, which can be used to extract baked gum; flowers can be used as a good source of honey, etc. It is precisely because chestnut has many important application values ​​and has a large market demand, so people pay more and more attention to the cultivation of its fine varieties. Especially at the beginning of the 20th century, the chestnut blight (Cryphonectria parasitica (Murr.) Barr) that occurred in the United States caused an unprecedented large-scale ecological environment disaster, and ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A01H4/00C12N5/04
Inventor 韩振海孙宁王忆许雪峰李天忠
Owner CHINA AGRI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap