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Rapid proliferation method of hosta undulata bailey

A flower-leaf hosta and fast technology, which is applied in the field of rapid proliferation of flower-leaf hosta, can solve the problems of undisclosed operating conditions, affecting industrial production, etc. Effect

Inactive Publication Date: 2014-06-25
INST OF BOTANY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the above-mentioned prior art discloses medium formulas at different stages, it does not disclose specific operating conditions, and the inventor operates according to the above-mentioned formula, and when using it for industrialized seedling breeding for large-scale multiplication, especially when multiple subcultures During the multiplication process, it was found that with the increase of the number of subcultures, the mutation rate increased rapidly, which seriously affected the factory production. Therefore, the inventors made targeted improvements

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] A method for rapid multiplication of hosta mosaic, comprising:

[0021] (1) Selection and disinfection of explants: excavate hosta rhizomes from March to April, wash them and plant them in flower pots with perlite as the substrate, only water tap water during the planting process, and take the sprouts after 30 days. For explants; immerse in a beaker filled with saturated soapy water for 5 minutes, then rinse with tap water until there is basically no foam, and then repeat the above soaking and rinsing steps once; soak in 70% alcohol for 1 minute in an ultra-clean bench, Rinse twice with sterile water, disinfect with 5% sodium hypochlorite solution containing two drops of Tween 80 for 10 minutes, rinse with sterile water for 5 times; then disinfect with 0.1% mercuric chloride containing two drops of Tween 80 for 10 minutes, Rinse with bacterial water 6 times;

[0022] (2) Inoculation culture: the explant described in step (1), peel off the outer layer of leaves, cut the...

Embodiment 2

[0027] A method for rapid multiplication of hosta mosaic, comprising:

[0028] (1) Selection and disinfection of explants: excavate hosta rhizomes from March to April, wash them and plant them in flower pots with perlite as the substrate, only water tap water during the planting process, and take the sprouts after 30 days. For explants; immerse in a beaker filled with saturated soapy water for 8 minutes, then rinse with tap water until there is basically no foam, and then repeat the above soaking and rinsing steps once; soak in 70% alcohol for 1 minute in an ultra-clean bench, Rinse twice with sterile water, disinfect with 5% sodium hypochlorite solution containing two drops of Tween 80 for 12 minutes, rinse with sterile water for 5 times; then disinfect with 0.1% mercuric chloride containing two drops of Tween 80 for 12 minutes, Rinse with bacterial water 6 times;

[0029] (2) Inoculation culture: the explant described in step (1), peel off the outer layer of leaves, cut the...

Embodiment 3

[0034] A method for rapid multiplication of hosta mosaic, comprising:

[0035] (1) Selection and disinfection of explants: excavate hosta rhizomes from March to April, wash them and plant them in flower pots with perlite as the substrate, only water tap water during the planting process, and take the sprouts after 30 days. For explants; immerse in a beaker filled with saturated soapy water for 10 minutes, then rinse with tap water until there is basically no foam, and then repeat the above soaking and rinsing steps once; soak in 70% alcohol for 1 minute in an ultra-clean bench, Rinse twice with sterile water, disinfect with 5% sodium hypochlorite solution containing two drops of Tween 80 for 15 minutes, rinse with sterile water for 5 times; then disinfect with 0.1% mercuric acid containing two drops of Tween 80 for 15 minutes, Rinse with bacterial water 6 times;

[0036] (2) Inoculation culture: the explant described in step (1), peel off the outer layer of leaves, cut the rh...

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PUM

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Abstract

The invention relates to a rapid proliferation method of hosta undulata bailey. The rapid proliferation method comprises the following main steps of selecting and disinfecting explants, carrying out subculture enrichment culture on the explants, carrying out rooting culture on the explants, and carrying out acclimatization and transplant on the explants. The rapid proliferation method provided by the invention has the advantages that in a subculture process, the illumination intensity is improved, the photoperiod is prolonged, the culture temperature is reduced, meanwhile, a technological method for transforming the subculture culture medium is adopted, and the good effects that the proliferation speed is rapid, the inheritable character is stable, and the proliferation coefficient is high are achieved.

Description

technical field [0001] The invention relates to a method for multiplying hosta, in particular to a method for rapidly multiplying hosta mosaic. Background technique [0002] Hosta mosaic (Hosta plantagineu) It is a plant of the genus Hosta in the family Liliaceae, a perennial root flower, and a variant of the famous foliage plant Hosta. It has strong complementarity to light-loving flowers and is an excellent variety for landscaping. The introduction and popularization of flower-leaf hosta has greatly improved its ornamental effect and enriched the landscape color of landscaping. The plant of this variety is short and shade-loving, with complex-colored leaves with folds, golden edges and silver hearts intertwined, and the colorful leaves present a magical cluster of flowers. It can be planted in places where the growth of sun-loving flowers is unfavorable, such as the back side of urban high-rise buildings, under the overpass, the shade of the big tree in the courtyard, an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 孙国峰张金政李晓东吴东启林秦文
Owner INST OF BOTANY CHINESE ACAD OF SCI
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